Int. J. Biosci.15( 4), 454-461, October 2019
Determination of microelements essential for pollen viability is important for it serves as a key for successful germination and propagation of species, particularly for threatened species. Thus, this study was conducted to determine the microelements that are crucial to Meistera muricarpa’s pollen germination and tube growth. The pollen grains were subjected into five different treatments, namely: T1 (No KCl); T2 (No MgSO4); T3 (No H3BO3); T4 (No Ca(No3)2), and T5 (control). Afterward, a hanging-drop method was carried out; samples were then observed for pollen germination and tube growth for ten hours with one-hour interval. Results revealed that the highest average percentage of pollen germination (PPG) and the longest pollen tube growth (PTG) were observed on T4 (17%; 72μm), attained at the 8th hour of the observation; while the lowest average percentage of germination and PTG were observed on T3 (4%; 33μm) on the 9th hour. As for the statistical analysis, the PPG in T1 to T3 was noted to be significantly different from the control medium while the result in T4 shows the opposite. While for the PTG, the results on T2 to T4 were revealed to have a significant difference against the control medium. Results imply that the microelements Boron and Magnesium in a form of Boric acid and Magnesium Sulfate are the most important microelements for M. muricarpa’s pollen germination and pollen tube growth.
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