Estimation of heterosis and combining ability for some quantitative parameters in Gossypium hirsutum

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Research Paper 01/08/2019
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Estimation of heterosis and combining ability for some quantitative parameters in Gossypium hirsutum

Muhammad Tanees Chaudhary, Sajid Majeed, Amir Shakeel, Jia Yinhua, Du Xiongming, Muhammad Tehseen Azhar
Int. J. Biosci.15( 2), 166-173, August 2019.
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Cotton is an important oilseed andfiber crop in Pakistan as well as in world. Improvement can be madein yield and fiber characteristics of cotton crop after understanding the various mechanisms of gene actions controlling the yield contributingtraits.In the current study, four genotypes of cotton namely, NIAB-KIRN, FH-942, PB-896 and PB-76 were crossed in a random mating fashion. The parents and F1 hybrids were planted in field in three replicationsfollowing randomize complete block design. At maturity, data were collected for yield and fiber related traits. Analysis of variance of mean valuesexhibitedthe presence of significant variations. This data were analyzed for their assessment of combining ability, where it is found that the genotype NIAB-KIRN has additive gene action for number of seeds/boll, seed index and seed cotton yield per plant. Thus, nominated as a good general combiner.Whereas PB-896 × PB-76 showed good specific combining ability for seed-cotton yield/ plant and cotton-seed yield per plant, while the combination of PB-896 × FH-942 has exhibited significantly high heterosis for fiber and seedcotton yield.Based on this information the parents and combinations have potential of genetic material for yield of seed cotton as well as fiber related parameters.


Arain BT, Baloch M J, Sial P, Arain MA, Baloch A. 2015. Estimation of heterosis and combining ability in F1 hybrids of upland cotton for yield and fibre traits. Biological Sciences  58, 132-139.

Ashokkumar  K, Ravikesavan  R, Prince  KSJ. 2010. Combining Ability Estimates for Yield and Fibre Quality Traits in Line X Tester Crosses of Upland Cotton,(Gossypium hirsutum). International journal of Biology 2, 179-187.

Aslam M, Sohail Q, Maqbool MA, Zaman QU, Zubair A. 2015. Combining ability analysis and genetic inheritance of salt tolerance indicators in maize (Zea mays) following diallel mating design. International journal of Agriculture and Biology 17, 23-34.

Azhar F, Ali Z, Akhtar M, Khan A, Trethowan R. 2009. Genetic variability of heat tolerance, and its effect on yield and fibre quality traits in upland cotton (Gossypium hirsutum L.). Plant Breeding 128, 356-362.

Bajracharya J. Steele K, Jarvis D, Sthapit B, Witcombe J. 2006. Rice landrace diversity in Nepal: Variability of agro-morphological traits and SSR markers in landraces from a high-altitude site. Field Crops Research 95, 327-335.

Bilwal B, Vadodariya K, Lahane G, Rajkumar B. 2018. Heterosis study for seed cotton yield and its yield attributing traits in upland cotton (Gossypium hirsutum L.). Journal of Pharmacognosy and Phytochemistry7, 1963-1967.

Chen ZJ, Scheffler BE, Dennis E, Triplett BA, Zhang T, Guo W, Town CD. 2007. Toward sequencing cotton (Gossypium) genomes. Plant physiology 145, 1303-1310.

Cothren JT, Oosterhuis D. 2010. Use of growth regulators in cotton production. Physiology of cotton 34, 289-303.

Coyle GG, Smith CW. 1997. Combining ability for within-boll yield components in cotton, Gossypium hirsutum L. Crop science 37, 1118-1122.

Ekinci R, Basbag S, Gencer O. 2010. Path coefficient analysis between seed cotton yield and some characters in cotton (Gossypium hirsutum L.). Journal of Environmental Biology 31, 861-876.

Esmail R, Zhang J, Abdel-Hamid A. 2008. Genetic diversity in elite cotton germplasm lines using field performance and RAPD markers. World Journal of Agriculture Sciences 4, 369-375.

Falconer D, Mackay T. 1996. Introduction to quantitative genetics. 1996. Harlow, Essex, UK: Longmans Green 3, 23-34.

Gilbert N. 1958. Diallel cross in plant breeding. Heredity 12, 477-482.

Green C, Culp T. 1990. Simultaneous improvement of yield, fiber quality, and yarn stregth in Upland cotton. Crop science 30, 66-69.

Griffing B. 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Australian journal of biological sciences 9, 463-493.

Ilyas M, Naveed M, Khan TM, Khan IA. 2007. Combining ability studies in some quantitative and qualitative traits of Gossypium hirsutum L. Journal of Agrıculture & Socıal Scıences 3, 39-42.

Jatoi WA, Memon S. 2016. Line x tester analysis for earliness and yield traits in cotton (gossypium hirsutum l.). Journal of Agricultural Research  54, 102-112.

Kannan N, Saravanan K. 2016. Research Article Heterosis and combining ability analysis in tetraploid cotton (G. hirsutum L. and G. barbadense L.). Electronic Journal of Plant Breeding  7, 520-528.

Khan NU, Hassan G, Marwat K, Kumbhar M, Khan I, Soomro Z, Khan M. 2009. Legacy study of cottonseed traits in upland cotton using Griffing’s combining ability model. Pakistan  Journal of Botany 41, 131-142.

Khokhar ES, Shakeel A, Maqbool MA, Abuzar MK, Zareen S, Syeda SA, Asadullah M. 2018. Studying combining ability and heterosis in different cotton (Gossypium hirsutum L.) genotypes for yield and yield contributing traits. Pakistan Journal of Agricultural Research 31, 233-240.

Kothari N, Campbell BT, Dever JK, Hinze LL. 2016. Combining ability and performance of cotton germplasm with diverse seed oil content. Crop science 56, 19-29.

Mahpara S, Ali Z, Rehmani M, Iqbal J, Shafiq M. 2017. Studies of genetic and combining ability analysis for some physio-morphological traits in spring wheat using 7× 7 diallel crosses. International  Journal of Agriculture and Applied Sciences 9(1), 33-40.

Maqboool M, Aslam M, Khan MS, Issa, A, Ahsan M. 2017. Evaluation of single cross yellow maize hybrids for agronomic and carotenoid traits. International Journal Of Agriculture And Biology 19(5), 1087-1098.

McCullagh P. 2018. Tensor Methods in Statistics: Monographs on statistics and applied probability: Chapman and Hall/CRC 23, 412-420.

Murtadha M, Ariyo O, Alghamdi S. 2018. Analysis of combining ability over environments in diallel crosses of maize (Zea mays). Journal of the Saudi Society of Agricultural Sciences 17, 69-78.

Patil S. 2018. Combining ability studies for seed cotton yield and its attributing characters in tetraploid cotton (G. hirsutum L.). IJCS, 6, 2022-2027.

Rauf S, Khan TM, Nazir S. 2005. Combining ability and heterosis in Gossypium hirsutum L. International  journal of. Agriculture. Biology 7, 109-113.

Samreen  K, Baloch M, Soomro  Z, Kumbhar M, Khan N, Kumboh  N, Veesar N. 2008. Estimating combining ability through Line× Tester analysis in upland Cotton (Gossypium hirsutum). Sarhad Journal of Agriculture 24, 581-586.

Sawarkar M, Solanke A, Mhasal G, Deshmukh S. 2015. Combining ability and heterosis for seed cotton yield, its components and quality traits in Gossypium hirsutum L. Indian Journal of Agricultural Research  49, 212-220.

Shabbir G, Husnain S, Mehdi S, Ehsan M. 2018. Combining ability studies in rice through 6 x 6 diallel cross analysis. Journal of Agricultural Research 55, 123-129.

Singh SA, Singh V V, Choudhary AD. 2009. Combining ability estimates for oil content, yield components and fibre quality traits in cotton (G. Hirsutum) using diallel mating design. Tropical and Subtropical Agroecosystems 12, 161-166.

Steel RGD, Torrie JH. 1980. Principles and procedures of statistics, a biometrical approach: McGraw-Hill Kogakusha, Ltd.

Tigga A, Patil S, Edke V, Roy U, Kumar A. 2017. Heterosis and inbreeding depression for seed cotton yield and yield attributing traits in intrahirsutum (G. hirsutum L. X G. hirsutum L.) hybrids of cotton. International Journal of Current Microbiology and Applied Sciences 6, 2883-2887.

Ukai Y, Dial. 98. A package of programs for the analyses of a full and half diallel table with the methods by Hayman (1954), Griffing (1954) and others. 2006. Available from:

Zeng L, Pettigrew WT. 2015. Combining ability, heritability, and genotypic correlations for lint yield and fiber quality of Upland cotton in delayed planting. Field Crops Research 171, 176-183.

Zhang J, Abdelraheem A, Wu J. 2017. Heterosis, combining ability and genetic effect, and relationship with genetic distance based on a diallel of hybrids from five diverse Gossypium barbadense cotton genotypes. Euphytica 9, 201-208.

Zhang J, Wu M, Yu J, Li X, Pei W. 2016. Breeding potential of introgression lines developed from interspecific crossing between upland cotton (Gossypium hirsutum) and Gossypium barbadense: heterosis, combining ability and genetic effects. PloS one 11, 64-69.