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Estimation of necrosis length, phenolic content, peroxidase and polyphenoloxidase activity in cocoa plants (Theobroma cacao L.) after Phytophthora megakarya inoculation

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Research Paper 01/03/2017
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Estimation of necrosis length, phenolic content, peroxidase and polyphenoloxidase activity in cocoa plants (Theobroma cacao L.) after Phytophthora megakarya inoculation

Martine Louise Ondobo, Pierre Effa Onomo, Jude Manga Ndjaga, Jules Christian Djoko Kouam, Pierre François Djocgoue, Denis Omokolo Ndoumou
Int. J. Agron. Agri. Res.10( 3), 26-36, March 2017.
Certificate: IJAAR 2017 [Generate Certificate]
Key: CocoaEnzymesHeterosisNecrosisPhytophthora megakarya

Abstract

Cocoa (Theobroma cacao L.) is the principal culture of income for many countries of the world. In Cameroon, black pod disease caused by Phytophthora megakarya is one of the most economically destructive diseases of cocoa. Hybridation is a reliable method of ameliorating cocoa production. The aim of this study was to select new genotypes with resistance to P. megakarya. Necrotic lesions, total polyphenols (TPP) and activities of antioxidant enzymes [peroxidase (POX) and polyphenoloxidase (PPO)] were conducted on leaves of three parental clones (T79/467, SNK413 and ICS40) and their hybrids (families F70, F30, F90 and F95) derived from reciprocal crossing after inoculation. 96% of the hybrid’s genotype manifested a positive heterosis effect for the development of lesion size suggesting the existence of hybrid vigour. The F30.03, F30.07, F70.04, F70.07, F90.03, F95.01, F95.08 and F95.11 genotypes showed a significant increase (P˂0.05) of biochemical components negatively correlated with the necrosis length and this increase was genotype-dependent. Those hybrids can be considered as elite clones. Furthermore, the pair of parental clones (T79/467-SNK413 and T79/467-ICS40) has showed good aptitudes for the combination of the characters studied and no maternal effect was detected in their transmission.

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