Evaluating the effects of Levonorgestrel on female gonads of Rattus albicans
Paper Details
Evaluating the effects of Levonorgestrel on female gonads of Rattus albicans
Abstract
Though the repeated or long term use of progesterone only birth control measures are found to be disruptors of reproductive cycle, it is often reported that the effects of an exogenous progesterone analog on the histology of reproductive organs cannot be predicted easily, which is utmost important for the users. Therefore, the present work is designed to enumerate whether a commonly used synthetic progestogen, Levonorgestrel (LNG) has disruptive effects on the ovarian histology at a dose of 1.5mg/kg b.w/day for 03 consecutive days at 24 h time interval in cyclic ovary-intact female rats.Present investigation reveals that administration of LNG reduces ovary sizes, causes detachment of the cumulus oophorous cells from the oocyte, changes the shape of the oocyte, increased follicular atresia along with increase in number of secondary follicles and decrease in numbers of primordial and grafiaan follicles. Number of corpus luteum was also decreases. From these results, it can be concluded that prolonged or repeated use of LNG may disrupt the ovarian activity by affecting folliculogenesis, rate of ovulation thereby the rate of fertility in females. Information from this study might help researchers to develop certain contraceptives from natural resources with little side effects rather than synthetic contraceptives.
Alvarez F, Brache V, Tejada AS, Faundes A. 1986. Abnormal endocrine profile among women with confirmed or presumed ovulation during long-term norplant use. Contraception 33, 111-20. http://dx.doi.org/10.1016/0010-7824(86)90077-6.
Banks EP, Jefferson WN, Newbold RR. 2001. The immature mouse is a suitable model for detection of estrogenicity in the uterotrophic bioassay. Environmental Health Perspectives 109, 821-26.
Benagiano G, Pera A, Primiero M. 2000. The endometrium and hormonal contraceptives.Human reproduction 15(1), 101-118. http://dx.doi.org/10.1093/humrep/15.suppl_1.101
Bhowmik T, Mukherjee M. 1988. Histological changes in the ovary and uterus of rat after injectable contraceptive therapy. Contraception 35(5), 530-34.
Brasil FB, Soares LL, Faria TS, Boaventura GT, Sampaio FJB, Ramos CF. 2009.The impact of dietary organic and transgenic soy on the reproductive system of female adult rat. The Anatomical Record 292(4), 587-94.
Butcher RL, Kirkpatric-Keller D. 1984. Pattern of follicular growth during the four-day estrous cycle in the rat. Biology of Reproduction 31, 280-86.
Chen Y, Shi X. 2016. Repeated use of mifepristone and levonorgestrel and their effect on the ovarian function in mice. Journal of Obstetrics and Gynaecology Research. ©2016 Japan Society of Obstetrics and Gynecology. http://dx.doi.org/10.1111/jog.13085.
Girija S, Kamakshi K. 2003. Experimental studies on the estrous cycle and histomorphometry of uterus and ovary in adult female albino rats subjected to injectable contraceptive treatment and swimming stress followed by polyherbal drug administration. Thesis, submitted to the University of Madras, faculty of Medicine (Anatomy) in the fulfillment for the degree of Doctor of Philosophy p-75.
Hatcher RA, Trussel J, Stewart F. 1988.Contra Tech. 1988-1989, 14th revision. Irvington Publishers, New York.
Hazarika P, kalita S, Choudhury K. 2021.Restorative Effects Of Saraca indica methanolic bark extract on Levonorgestrel affected reproductive cycle and hormone levels in female albino Rats. Uttar Pradesh Journal of Zoology 42(3), 15-22.
Heikinheimo O, Gordon K, Williams RF, Hodgen GD. 1996. Inhibition of ovulation by progestin analogs (agonists Vs antagonists): preliminary evidence for different sites and mechanism of actions. Contraception 53(1), 55-64.
Hui XLV, Zhao DSHI. 2011. Effects of Levonorgestrel on Reproductive Hormone Levels and Their Receptor Expression in Mongolian Gerbils (Merione sunguiculatus). Experimental Animals. 60(4), 363–371.
Jefferson WN, Padilla-Banks E, Newbold RR. 2005. Adverse effects of female development and reproduction in CD-1 mice following neonatal exposure to the phytoestrogen genistein at environmentally relevant doses. Biology of Reproduction 73, 798–806.
Johnson MH, Everitt BI. 2000. Essential Reproduction, 5th edition. p-85. By Blackwell Science Ltd.
Kurian A, KaushikK, Subeesh V, Maheswari E, Kunnavil R. 2018. Safety profile of LNG: A disproportionality analysis of Food and Drug administration Adverse Event Reporting System (Faers) Database. Journal of Reproduction and Infertility 19(3), 152-156.
Larsson CU, Berlin R, Vikrot O. 1970.Effects of combined and low dose Gestagen oral contraceptives on plasma lipids; including phospholipids. Acta Endocrinologia 63, 717-35.
Manikkam M, Rajamahendran R. 1997. Progesterone–induced atresia of the proestrus dominant follicle in the bovine ovary: changes in diameter, insulin-like growth factor system, aromatase activity, steroid hormones, and apoptotic index. Biology of Reproduction 57, 580-87.
Margarete ADVM, Peter ADVM, Andreas R, Daniela S, Thomas R. 2001. Optimization of intraperitonial anesthesia in mice: drugs, dosages, adverse effects and anesthesia depth. Comparative Medicine 5, 443-456.
Martin L, Finn CA, Carter J. 1970. Effects of Progesterone and Oestradiol-17β on the luminal epithelium of the mouse uterus. Journal of Reproduction and Fertility 21, 461-69.
Martinez- Manautou J, Aznar R. 1975. Endometrial morphology in women exposed to uterine systems releasing progesterone. American Journal of Obstetrics and Gynecology 121(2), 175-79.
Mazur M, Kurman RJ. 2005. Diagnosis of endometrial biopsies and curettings: a practical approach. 2nded. New York: springer Science and Business Media, Inc.
Meyers M, Britt KL. 2004. Methods for quantifying follicular numbers within the mouse ovary. Reproduction 127, 569-580.
The ESHRE Capri Workshop Group. 2001. Ovarian and endometrial function during hormonal contraception. Human Reproduction 16(7), 1527-1537.
OECD 407. 2008. OECD guidelines for the testing of chemicals, repeated dose 28-day oral toxicity study in rodents. Organization for Economic Cooperation and Development, Paris. https://doi.org/10.1787/9789264070684-en
Pedersen T, Peters H. 1968. Proposal for a classification of oocyte and follicles in the mouse ovary. Journal of Reproduction and Fertility 17, 555-557.
Pritis EA, Ryan IP, Mueller MD, Lebovic DI, Shifren JL, Zaloudek CJ, Korn AP, Darney PD, Taylor RN. 2005. Angiogenic Effects of Norplant Contraception on Endometrial Histology and Uterine Bleeding. Journal of Clinical Endocrinology and Metabolism 90(4), 2142-2147.
Rawal S. 2016. Progestogenic effect of norethisterone on uterine chemistry and uterine endometrium ultra-structure of albino rat (wistar strain). European Journal of Biotechnology and Biosciences. 4(7), 29-33. www.biosciencejournals.com
Scudamore CL. 2014. A practical guide to the histology of the mouse.John Wiley and Sons Publication, First edition 87-107.
Segal SJ, Faundes A, Alvarez-Sanchez F, Vilja P, Brache V, Tuohimaa P. 1991. Norplant implants: the mechanism of contraceptive action. Fertility and Sterility 56, 273-277.
Speroff L, Darney PD. 1992. A Clinical Guide for Contraception. Williams and Williams, Baltimore.
Palki Hazarika, Kamal Choudhury, 2021. Evaluating the effects of Levonorgestrel on female gonads of Rattus albicans. Int. J. Biosci., 19(4), 108-115.
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