Evaluation of antioxidant activity of lime juice extract of cashew bark in normal and wistar rats with indomethacin toxicity

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Research Paper 21/02/2024
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Evaluation of antioxidant activity of lime juice extract of cashew bark in normal and wistar rats with indomethacin toxicity

Godwin Anyim, Morakinyo Adetoun Elizabeth, Babarinde Samuel Olufolarin, Bamgboye Faith Funke, Akinbinu Ibukunoluwa Rebecca, Oyedemi Temitope, Oyedepo Temitope Adenike
Int. J. Biomol. & Biomed.18( 1), 26-37, February 2024.
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Abstract

This study evaluated the effect of lime juice extract of cashew bark (LJECB) on serum antioxidant status in normal and Wistar rats with indomethacin toxicity. Fresh Anacardium occidentale bark was pulverised and extracted using fresh juice of Citrus aurantifolia. Forty two female Wistar rats (150-200 g) were purchased and randomised into seven groups (n=6): Group 1 (normal control); Group 2 (Indomethacin 30 mg/kg only); Group 3 (Indomethacin 30 mg/kg + Gecrol antacid); Group 4 (Indomethacin 30 mg/kg + LJECB 400 mg/kg); Group 5 (Indomethacin 30 mg/kg + LJECB 800mg/kg); Group 6 (LJECB 400 mg/kg only), Group 7 (LJECB 800 mg/kg only). Post-treatment with LJECB or Gecrol antacid was commenced 4 hours for 14 days. On the 15th day, the animals were sacrificed, and the serum was collected aand used for antioxidant, lipid peroxidation (malonialdehyde MDA), and total protein assays based on standard protocols. Results showed that treatment with 400 mg/kg LJECB elevated the nitric oxide (NO), superoxide dismutase (SOD), and MDA levels, but lowered the activities of glutathione peroxidase (GPx) and catalase (CAT). Also, 800 mg/kg of LJECB elevated the NO, CAT, SOD, and MDA levels, but lowered the level of GSH, GPx, and total protein. The study concludes that LJECB provoked a significant increase in antioxidant status (NO, SOD, and CAT) but lowered the activity of GPx and reduced glutathione (GSH). However, the elevation of lipid peroxidation biomarker (MDA) suggests a possible damage to the stomach endothelial membranes. Therefore, caution should be exercised when consuming the LJECB for therapy.

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