Evaluation of antioxidant and antibacterial activity of Putranjiva roxburghii Wall. fruit peel

Paper Details

Research Paper 01/07/2019
Views (262) Download (11)

Evaluation of antioxidant and antibacterial activity of Putranjiva roxburghii Wall. fruit peel

Md. Mahmudul Hasan, Most. Sayla Tasmin, Md Abu Reza, Ariful Haque
Int. J. Biosci.15( 1), 397-403, July 2019.
Certificate: IJB 2019 [Generate Certificate]


Putranjiva roxburghii Wall. (Euphorbiaceae) has long been used in folk treatment. Considering this folkloric background, this study was designed to evaluate in vitro antioxidant and antibacterial activity of P. roxburghii fruit peel (PRFP) enthanolic (95%) extract. Antioxidant activity was checked based on 2, 2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging capacity. Antibacterial aptitude of PRFP was assessed using disc diffusion assay against two bacterial strains Bacillus subtelis and Enterobacter xiangfangensis. Disc diffusion assay was performed with three different PRFP doses (250, 500, and 1000 µg/disc). PRFP showed excellent antioxidant activity with 17.3 µg/mL IC50 (concentration that scavenged 50% DPPH radical) value. In antibacterial test, PRFP inhibited growth both of B. subtelis and E. xiangfangensis by revealing inhibition zone in dose-dependent fashion. Overall, PRFP is a rich source of pharmaceutical agents in terms of antioxidant and antibacterial activity.


Al-Abd NM, Nor ZM, Mansor M, Azhar F, Hasan MS, Kassim M. 2015. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract. BMC Complementary and Alternative Medicine 15, 385-394. https://doi.org/10.1186/s12906-015-0914-y

Al-Rifai A, Aqel A, Al-Warhi T, Wabaidur SM, Al-Othman ZA, Badjah-Hadj-Ahmed AY. 2017. Antibacterial, antioxidant activity of ethanolic plant extracts of some Convolvulus species and their DART-ToF-MS profiling. Evidence-Based Complementary and Alternative Medicine 2017, 1-9. https://doi.org/10.1155/2017/5694305

Bandara KR, Padumadasa C, Peiris DC. 2018. Potent antibacterial, antioxidant and toxic activities of extracts from Passiflora suberosa L. leaves. PeerJ 6, e4804. http://dx.doi.org/10.7717/peerj.4804

Boonyaprapat N, Chokechaicharoenporn A. 1999. Samunprai Maipuenban. Prachachon, Bangkok 3, 262-273.

Chaudhary NS, Shee C, Islam A, Ahmad F, Yernool D, Kumar P, Sharma AK. 2008. Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii seeds. Phytochemistry 69(11), 2120-2126. http://dx.doi.org/10.1016/j.phytochem.2008.05.002

Hasan MM, Hasan R, Al Mahmud MR, Islam MG. 2018. Protective effect of Citrus macroptera fruit pulp juice rendering Nrf2-pathway against cisplatin-induced genotoxicity. Journal of Applied Pharmaceutical Science 8(12), 67-71. http://dx.doi.org/10.7324/JAPS.2018.81209

Hasan MM, Mahmud MRA, Islam MG. 2019. GC-MS Analysis of Bio-active Compounds in Ethanol Extract of Putranjiva roxburghii Wall. Fruit Peel. Pharmacognosy Journal 11(1), 146-149. http://dx.doi.org/10.5530/pj.2019.1.24

Hassine M, Zardi-Berguaoui A, Znati M, Flamini G, Ben Jannet H, Hamza MA. 2014. Chemical composition, antibacterial and cytotoxic activities of the essential oil from the flowers of Tunisian Convolvulus althaeoides L. Natural Product Research 28(11), 769-775. http://dx.doi.org/10.1080/14786419.2013.879476

Joty FA, Hasan MM, Khatun R, Billah MM, Miah MM, Mahmud S, Reza MA, Ferdousi Z. 2019. Evaluation of antibacterial and antioxidant activity of three plant species from Morus genus. International Journal of Bioscience 14(2), 183-189. http://dx.doi.org/10.12692/ijb/14.2.183-189

Khan FA, Hussain I, Farooq S, Ahmad M, Arif M, Rehman IU. 2011. Phytochemical screening of some Pakistanian medicinal plants. MiddleEast Journal of Scientific Research 8(3), 575-578.

Kumari R, Kumar S, Kumar A, Goel KK, Dubey RC. 2017. Antibacterial, antioxidant and Immuno-modulatory properties in extracts of Barleria lupulina Lindl. BMC Complementary and Alternative Medicine 17(1), 484-492. https://doi.org/10.1186/s12906-017-1989-4

Okwu DE, Okwu ME. 2004. Chemical composition of Spondias mombin Linn. Plant parts. Journal of Sustainable Agriculture and the Environment 6(2), 140-147.

Parekh J, Nair R, Chanda, S. 2005. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian Journal of Pharmacology 37(6), 408-409. http://dx.doi.org/10.4103/0253-7613.19085

Patel GK, Kar B, Sharma AK. 2012. Characterization of a thermostable family 1 Glycosyl Hydrolase enzyme from Putranjiva roxburghii seeds. Applied Biochemistry and Biotechnology 166(3), 523-535. http://dx.doi.org/10.1007/s12010-011-9445-2

Phuphathanaphong L. 2006. Flor a of Thailand Euphorbiaceae. National Herb Nederland 336, 1877-1887.

Raquel FE. 2007. Bacterial lipid composition and antimicrobial efficacy of cationic steroid compounds. Biochemica et Biophysica Acta 4(3), 250-259. http://dx.doi.org/10.1016/j.bbamem.2007.05.023

Raza M, Fozia RA, Wahab A, Iqbal H, Ullah H, Ahmad S, Ahmad I, Shah SM. 2012. Comparative antibacterial study of Convolvulus arvensis collected from different areas of Khyber Pakhtunkhwa, Pakistan. International Research Journal of Pharmacy 3(10), 220-222.

Reanmongkol W, Noppapan T, Subhadhirasakul S. 2009. Antinociceptive, antipyretic, and anti-inflammatory activities of Putranjiva roxburghii Wall. Leaf extract in experimental animals. Journal of Natural Medicine 63(3), 290-299. http://dx.doi.org/10.1007/s11418-009-0336-6

Singh H, Bisht GS. 1999. Some novel folk treatments among the Tribes of Uttar Pradesh. Ancient Science of Life 18(3-4), 250-253.

Velioglu Y, Mazza G, Gao L, Oomah B. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 46(10), 4113-4117. http://dx.doi.org/10.1021/jf9801973

Yadav RN, Agarwala M. 2011. Phytochemical analysis of some medicinal plants. Journal of Phytology 3(12), 10-14.