Evaluation of larvicidal potential of Azadirachta indica (Neem) plant extract and synthesized AgNPs against Aedes aegypti L. (Diptera: Culicidae)

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Research Paper 01/04/2019
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Evaluation of larvicidal potential of Azadirachta indica (Neem) plant extract and synthesized AgNPs against Aedes aegypti L. (Diptera: Culicidae)

Muhammad Waris, Shabab Nasir, Farhat Jabeen, Tayyaba Sultana
Int. J. Biosci.14( 4), 26-34, April 2019.
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Abstract

Aedes mosquitoes are the most important group of vectors having ability to cause diseases like Chikungunya fever, Dengue fever and Zika virus in human. These vectors can efficiently and ecofriendly be controlled by using green silver nanoparticles (AgNPs). In the present study, leaves extract of Azadirachta indica (Neem) and green silver nanoparticles (AgNPs) synthesized from this extract were evaluated as larvicidal agent for 2nd  and 3rd instar larvae of the Aedes aegypti ( main mosquito vectors). UV-Vis spectroscopy, X-ray spectroscopy (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to study the characteristics of green synthesized AgNPs.2nd and 3rd instar larvae of Aedes aegypti were kept in the different concentrations (50-250ppm) of plant extract and green AgNPs to calculate the percentage mortality at time intervals of 12, 24, 36 and 48h of exposure. The nanoparticles proved significant toxic for the Ae. aegypti larvae (dengue vector) having (LC50 =15.94 and LC90 =111.82 ppm) compared to the plant extract (102.46 ppm; 251.80 ppm) respectively. Our results suggest the extract of A. indica and synthesized nanoparticles as excellent controlling agents for vector mosquitoes instead of pollution causing existing chemical pesticides.

VIEWS 16

Ahmad T, Nasir S, Nasir I, Nawaz T, Rafique, Yousaf I. 2017. Response surface modeling for West Nile viral encephalitis mosquito control experiments. Pakistan Veterinary Journal 37, 465‒469.

Ahmed S, Ahmad M, Swami BL, Ikram S. 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Journal of Advanced Research 7, 17–28. https://doi.org/10.1016/j.jare.2015.02.007

Al-Mekhlafi FA. 2018. Larvicidal ovicidal activities and histopathological alterations induced by Carumcopticum(Apiaceae) extract against Culex pipiens(Diptera: Culicidae). Saudi Journal of Bioloical Sciences 25, 52-56. https://doi.org/10.1016/j.sjbs.2017.02.010

Arivoli S, Ravindran KJ, Raveen R, Tennyson 2012. S. Larvicidal activity of botanicals against the filarial vector Culex quinquefasciatusSay (Diptera: Culicidae). International Journal of Zoological Research 2, 13– 17.

Arjunan N, Murugan K, Rejeeth C, Madhiyazhagan P, Barnard D. 2012. Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue. Vector Borne Zoonotic Diseases 12, 262–268. https://doi.org/10.1089/vbz.2011.0661.

Azhari HN, Jessinta, Abdurahman HN. 2012. Larvicidal activity of extracts from different parts of Neem (Azadirachta indica) against Aedes Aegypti mosquitoes’ larvae. Scientific Research Essays 7, 2810-2815.

Bilal H, Hassan SA. 2012. Plants secondary metabolites for mosquito control. Asian Pacific Journal of Tropical Diseases 2, 168-168.

Borase H, Patil C, Patil R. 2013. Phyto-synthesized silver nanoparticles: a potent mosquito biolarvicidal agent. Journal of Nanomedicine & Biotherapeutic Discovery 3, 1.

Busi S, Hnamte S, Rajkumari J. 2015. Biogenic synthesis of silver nanoparticles using aqueous floral extract of Azadirachta indica and its Anti-Candida and larvicidal activities. Research Journal of Chemical Environment 19, 10-13.

Darwish MA, Hoogstraal H, Roerts TJ, Ahmed IP, Omar F. 1983. A sero-epidemiological survey for certain arboviruses (Togaviridae) in Pakistan. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 442-445.

Eliman MA, Elmalik KH, Ali FS. 2012. Efficacy of leaves extract of CalotropisproceraAit. (Asclepiadaceae) in controlling Anopheles arabiensisand Culex quinoquefasciatusmosquito. Saudi Journal of Biological Sciences 16, 95-100. https://doi.org/10.1016/j.sjbs.2009.10.007.

Ghosh A, Chowdhury N, Chandra G. 2012. Plant extracts as potential mosquito larvicides. Indian Journal of Medicinal Plants Research 13, 581-598.

Isman BM. 2006. Botanical insecticides deterrents and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomolology 51, 45-66.

Jahan F. 2011.Dengue Fever (DF) in Pakistan. Asia Pacific Family Mededicines 10, 1. http://dx.doi.org/10.1186/1447-056X-10-1.

Khair-ul-Bariyah S, Ahmed D, Ikram M. 2012. Ocimumbasilicum: a review on phytochemical and pharmacological studies. Pakistan Journal of Chemistry 2, 78-85.

Mdoe FP, Cheng SS, Msangi S, Nkwengulila G, Chang ST, Kweka EJ. 2014. Activity of Cinnamomumosmophloeumleaf essential oil against Anopheles gambiae .Parasite Vectors 7, 209.

Muthukumaran U, Govindarajan U, Rajeswary M, Hoti S. 2015. Synthesis and characterization of silver nanoparticles using Gmelinaasiaticaleaf extract against filariasis, dengue, and malaria vector mosquitoes. Parasitology Research 114, 1817–1827.

Pavela R. 2016. History presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects. A review. Plants Protection Science 52, 229-241. https://doi.org/10.17221/31/2016-PPS

Poopathi S, De Britto LJ, Praba VL, Mani C, Praveen M. 2015. Synthesis of silver nanoparticles from Azadirachta indica a most effective method for mosquito control. Environmental Sciences of Pollution Research 22, 2956-63. https://doi.org/10.1007/s11356-014-3560-x.

Qasim M, Naeem M, Bodlah I. 2014. Mosquito (Diptera: Culicidae) of Murree Hills, Punjab, Pakistan. Pakistan Journal of Zoology 46, 523-529.

Rauf M, Fatima-tuz-Zahra, Sobia M, Azra M, Shameem B. 2017.Outbreak of chikungunya in Pakistan. Correspodence 17, 258.

Ravikumar G, Rahuman A. 2011. Larvicidal activity of synthesized silver nanoparticles using Ecliptaprostrataleaf extract against filariasis and malaria vectors. ActaTropica 118, 196–203. https://doi.org/10.1016/j.actatropica.2011.03.003.

Remia KM, Logaswamy S. 2010. Larvicidal efficacy of leaf extract of two botanicals against the mosquito vector, Aedes aegypti (Diptera: Culicidae). Indian Journal of Natural Products and Resources 1, 208-212

Satyavani K, Ramanathan T, Gurudeeban S. 2011. Plant mediated synthesis of biomedical silver nanoparticles by leaf extract of Citrulluscolosynthis.  Research Journal of Nano Technology 1, 95-101.

Sergio MV, Alberto AI, Luis MR, Humberto VD, Jesus BF. 2007. Neem Tree Morphology and Oil content. Issues in new crops and new uses 1, 126-128.

Service M. 2004. Medical Entomology for Students.3rd eddition Cambridge, UK, Cambridge University Press.

Veerakumar K, Govindarajan M, Rajeswary M, Muthukumaran U. 2016. Mosquito larvicidal properties of silver nanoparticles synthesized using Heliotropiumindicum (Boraginaceae) against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae). Parasitology Research 113, 2363-2373. https://doi.org/10.1007/s00436-014-3895-8

Virendra KD, Akhilesh CP, Kamaraju R, Ashish G, Trilochan S, Aditya PD. 2009. Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoes.Malaria Journal 8, 124. https://doi.org/10.1186/1475-2875-8-124

Vogel AI. 1978. Text Book of practical organic chemistry. The English Language Book     Society and Longman, London, 1368.

WHO. 2006. Pesticides and their application for the control of vectors and pests of public health importance. Geneva, Switzerland, World Health Organization.

Zhu J, Zeng X, Neal OM, Schultz G. 2008. Mosquito larvicidal activity of botanical based mosquito repellents. Journa of Amerecan Mosquito Control Assocociation 2, 161- 168. 10.2987/8756971X(2008)24[161:MLAOBM]2.0.CO;2