Evaluation of phytocompounds from Azima tetracantha using UV-VIS and FTIR analysis

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Research Paper 05/08/2025
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Evaluation of phytocompounds from Azima tetracantha using UV-VIS and FTIR analysis

R. Devi Anbarasi, V. Ramamurthy
Int. J. Biosci. 27(2), 69-75, August 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

The aim of this research work is to evaluate the pharmacological potentials of the Azima tetracantha. Phytochemical, antioxidant and antibacterial properties of ethanolic extract were carried out. For UV-VIS spectrophotometric analysis, the ethanolic extract of Azima tetracantha was scanned in the wavelength ranging from 250‐900 nm by using Perkin Elmer Spectrophotometer and the characteristic peaks and their absorption values were detected. For FTIR Analysis, the ethanolic extract of Azima tetracantha was focused in the transmittance ranging between 400-4000 cm-1 on a spectrophotometer system and the characteristic peak values and their functional groups were detected. The UV‐VIS profile showed the peaks at 211.15, 213.01, 354.40 and 470.64 nm with the absorption values 2.6812, 2.6821, 0.21950 and 0.15169 respectively. The result of UV-VIS spectroscopic analysis confirms the presence of phenols and flavonoids in the Azima tetracantha extract. The results of the present FTIR study confirms the presence of Phenol, Alkane, Alkene, Carboxylic acid, Aromatic compound, Nitro compound, Alcohol, Benzene and Bromo alkanes compounds. The selected sample has a high inhibitory performance like antimicrobial activity against fungal species. Since bioactive compounds occurring in Azima tetracantha have broad spectrum like phenols, flavonoids, anti-inflammatory, hypocholesterolemic, cancer preventive, hepatoprotective, nematicide insectifuge, antihistaminic etc. Moreover help to identify the natural compounds from wild mushroom.

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