Evaluation of probiotic potential of Lactobacillus isolated from indigenous plant sources

Paper Details

Research Paper 01/03/2019
Views (616)
current_issue_feature_image
publication_file

Evaluation of probiotic potential of Lactobacillus isolated from indigenous plant sources

Sidra Riaz, Muhammad Ashraf, Riffat Shamim Aslam
Int. J. Biosci. 14(3), 470-476, March 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Probiotics are non-pathogenic, live micro-organisms which confer beneficial effects to host when administered in adequate amount. Lactococcus and Lactobacilli are representative genera of probiotic lactic acid bacteria (LAB). The present study was conducted to isolate Lactobacillus species from indigenous plant sources, characterize them and evaluate their Probiotic potential. A total of 30, samples from different plant sources were processed for the isolation of Lactobacilli. Samples were divided in to three groups: Group I (fruits), Group II (vegetables) and Group III (miscellaneous). Out of 30 samples only 4 samples were positive for Lactobacilli. Lactobacilli were isolated on de Man Ragosa and Sharpe (MRS) agar and identified on the basis of macroscopic, microscopic observations and biochemical tests. Isolates from strawberry, tomato and coriander samples were identified as Lactobacillus plantarum. One of the isolates from tomato was identified up to genus level only. Probiotic potentials such as resistance to low pH, bile salt and gastric juice were evaluated by viable colony count. Antibiotic susceptibility testing (AST) and antimicrobial activity were evaluated by agar diffusion methods. Isolates were resistant to pH 4, bile salt and gastric juice. Isolates were resistant to Penicillin G, susceptible to Sulfamethoxazole trimethoprim, gave intermediate results against Oxytetracycline and variable results against Pipemidic acid. All the indicator pathogens were significantly inhibited by the isolates (P< 0.05). The results of study revealed that L. plantarum is most abundant Lactobacillus isolated from indigenous plant sources. Indigenous fruits are good source of Lactobacillus than indigenous vegetables. L. plantarum showed good probiotic potential.

Ahmed T, Kanwal R. 2004. Biochemical characteristics of lactic acid producing bacteria and preparation of Camel milk cheese by using starter culture. Pakistan Veterinary Journal 24, 87-91.

Amin M, Jorfi M, Khosravi AD, Samarbafzadeh AR, Farajzadeh Sheikh A. 2009. Isolation and Identification of Lactobacillus casei and Lactobacillus plantarum from plants by PCR and detection of their antibacterial activity. Journal of Biological Sciences 9, 810-814. http://dx.doi.org/10.3923/jbs.2009.810.814

Anacarso I, Basssoli L, Sabia C, Iseppi R, Condò C. 2015. Isolation and identification of lactic acid bacteria from plants and other vegetables matrices and microbial recombination with Enterococcus spp. American Research Thoughts 1, 1503-1515.

Balamurugan R, Chandragunasekaran AS, Chellappan G, Rajaram K, Ramamoorthi G , Ramakrishna BS. 2014. Probiotic potential of lactic acid bacteria present in homemade curd in southern India. The Indian Journal of Medical Research 140, 345-355.

Bamidele TA, Adeniyi BA, Ogunbanwo ST, Smith SI, Omonigbehin EA. 2011. Antibacterial Activities of Lactic Acid Bacteria Isolated from Selected Vegetables Grown in Nigeria: A Preliminary Report. Sierra Leone Journal of Biomedical Research 3, 128-132.

Demirer S, Ulusu NN, Aslim B, Kepenekci I, Ulusoy C, Andrieu MN, Erkek B, Aydintug S. 2007. Protective effects of Lactobacillus delbrueckii subsp bulgaricus B3 on intestinal enzyme activities after abdominal irradiation in rats. Nutrition Research 27, 300-305. http://dx.doi.org/10.1016/j.nutres.2007.02.003

FAO/WHO. 2001. Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Proc “Joint FAO/WHO Experts Consultations on evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria”, Córdoba, Argentina; 1-4 October 2001.

Felis GE, Dellaglio F. 2007. Taxonomy of Lactobacilli and Bifidobacteria. Current issues in Intestinal Microbiology 8, 44-61.

Garcia EF, Luciano WA, Xavier DE, da Costa WC, de Sousa Oliveira K, Franco OL, de Morais Júnior MA, Lucena BT, Picão RC, Magnani M, Saarela M, de Souza EL. 2016. Identification of Lactic Acid Bacteria in Fruit Pulp Processing Byproducts and Potential Probiotic Properties of Selected Lactobacillus Strains. Frontiers in Microbiology 7, 11 pages. https://doi.org/10.3389/fmicb.2016.01371

Garzón K, Ortega C, Tenea GN. 2017. Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Native Fruits of Ecuadorian Amazon. Polish Journal of Microbiology 66, 473-481. https://doi.org/10.5604/01.3001.0010.7037

Lawal RT, Eniola KIT, Adepeju AB, Oloketuyi SF, Oyeleke GO. 2016. Probiotic Potential of Lactic Acid Bacteria associated with African Star Apple (Chrysophyllum albidum). EPH- International Journal of Agriculture and Environmental Research 2, 93-101.

Manzoor A, Haq IU, Baig S, Qazi JI, Seratlic S. 2016. Efficacy of locally isolated lactic acid bacteria against antibiotic resistant uropathogens. Jundishapur. Journal of Microbiology 9, e18952.

Naeem M, Ilyas M, Haider S, Baig S, Saleem M. 2012. Isolation characterization and identification of Lactic acid bacteria from fruit juices and their efficacy against antibiotics. Pakistan Journal of Botany 44, 323-328.

Pundir RK, Rana S, Kashyap N, Kaur A. 2013. Probiotic potential of lactic acid bacteria isolated from food samples: an in vitro study. Journal of Applied Pharmaceutical Science 3, 85-93.

Shafakatullah N, Chandra M. 2015. Isolation of lactic acid bacteria from Allium cepa var.aggregatum and study of their probiotic properties. International Journal of Pharma Sciences and Research 6, 749-757.

Sofowora A, Ogunbodede E, Onayade A. 2013. The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional, Complementary and. Alternative Medicines 10, 210-229.

Tambekar DH, Bhutada SA. 2010. An evaluation of probiotic potential of Lactobacillus sp. from milk of domestic animals and commercial available probiotic preparations in prevention of enteric bacterial infections. Recent Research in Science and Technology 2, 82-88.

Tariq A, Mussarat S, Adnan M, Abd_Allah EF, Hashem A, Alqarawi AA, Ullah R. 2015. Ethnomedicinal Evaluation of Medicinal Plants Used against Gastrointestinal Complaints. BioMed Research International vol. 2015, 14 pages. http://dx.doi.org/10.1155/2015/892947

Trias R, Baneras L, Badosa E, Montesinos E. 2008. Bioprotection of Golden delicious apples and Iceberg lettuce against foodborne bacterial pathogens by lactic acid bacteria. International Journal of Food Microbiology 123, 50-60.

Yousaf Z, Wang Y, Baydoun E. 2013. Phytochemistry and Pharmacological studies on Solanum torvum Swartz. Journal of Applied Pharmaceutical Science 3, 152-160.

Related Articles

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.

Agronomic performance and profitability of coffee wildlings using different soil media mixtures

Maribel L. Fernandez, Ricardo B. Casauay, Ronel A. Collado, Int. J. Biosci. 27(2), 189-199, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 176-188, August 2025.

Design and development of solar powered water sprayer: A green technology innovation

Lorenzo V. Sugod, Int. J. Biosci. 27(2), 159-175, August 2025.

Knowledge, attitudes, practices, and social awareness regarding SARS-CoV-2 infection in the kyrgyz population in the post-pandemic period

Mirza Masroor Ali Beg, Haider Ali, Yahya Nur Ahmed, Yavuz Gunduz, Hafsa Develi, Tilekeeva UM, Int. J. Biosci. 27(2), 151-158, August 2025.

Tumor suppressing ability of myrtenal in DMBA-induced rat mammary cancer: A biochemical and histopathological evaluation

Manoharan Pethanasamy, Shanmugam M. Sivasankaran, Saravanan Surya, Raju Kowsalya, Int. J. Biosci. 27(2), 141-150, August 2025.

Assessing tree diversity in cashew plantations: Environmental and agronomic determinants in buffer zones of Mont Sangbé National Park, western Côte d’Ivoire

Kouamé Christophe Koffi, Kouakou Hilaire Bohoussou, Serge Cherry Piba, Naomie Ouffoue, Sylvestre Gagbe, Alex Beda, Adama Tondossama, Int. J. Biosci. 27(2), 122-133, August 2025.