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Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in outpatients among Mongo communities, Chad

Research Paper | April 1, 2019

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F. H. Yandai, G. Ndoutamia, B. N. Ngandolo, N. Bessimbaye, N. Barro

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Int. J. Micro. Myco.9( 1), 1-7, April 2019


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In recent years, the world has seen a surge in extended-spectrum β-lactamase-producing-bacteria. However, data on the dissemination of ESBL-producing Enterobacteriaceae in the community is not available in Chad. This study aims to determine the prevalence and antibiotic susceptibility pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in fecal carriage from outpatients. From September 2017 to February 2018, 102 stools samples collected were sent at IRED. All stool samples were seeded onto Mac Conkey agar plates supplemented with cefotaxim (CTX, 2μg/mL), subjected to standard bacteriological method for isolation and characterization. Susceptibility to antibiotics was tested according to Kirby Bauer disk methods in respect to European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2014). Out of the 102 samples investigated, 53 isolates were identified as Escherichia coli strains (84.9%) and K. pneumoniae (15.1%).Moreover, among the 53 strains, 33 (62.3%) belonged to extended spectrum β-lactamase producing (ESBL) group. The maximum resistance were observed with amoxicillin and clavulanic acid (82, 22%-87, 5%), nalidixic acid (93, 33%-100%), ciprofloxacin (71, 11%-75%), and gentamicin (80.00%-87.5%). K. pneumonia resistance to fosfomycin was significant (P = 0.011) than E. coli. Mostly isolates tested were sensitive to imipenem. This result shows the high rate of ESBLs-producing isolates among outpatients in the community of Mongo. Surveillance of antimicrobial resistance needs to be implemented in Chad to tailor interventions targeted at stopping the dissemination of ESBL producing Enterobacteriaceae.


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Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in outpatients among Mongo communities, Chad

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