Foot-and-mouth disease outbreaks investigations at Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013

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Foot-and-mouth disease outbreaks investigations at Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013

Umer Farooq, Khalid Naeem, Aamer Bin Zahur, Muhammad Azeem Khan, Umer Sidique
Int. J. Biosci.11( 5), 346-356, November 2017.
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Abstract

This study reports characterization of Foot-and-Mouth Disease Virus (FMDV) from clinical suspected cases collected from Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013. The attempts were made for the recovery FMDV in LFBK cell line and the viruses were confirmed by real time PCR and serotyped by indirect sandwich ELISA. Overall ELISA confirmed 51.2% FMD outbreaks. A total of 39 viruses (45.4%) were isolated from outbreaks which include sero-type O (35.9%), A (28.2%) and Asia-1 (35.9%). FMDV sero-type O isolates were characterized within the PanAsia2 and the sub lineage identified was PanAsia2 ANT-10 & PanAsia2 BAL-09. FMDV sero-type A was grouped within the lineage A/Asia/Iran-05. The sub lineage identified within FMDV Iran-05 was HER-10, FAR-11 & ESF-10. FMDV sero-type Asia-1 was classified in group VII (Sindh-08). The diagnostic sensitivity of ELISA was found to be 61.54% (95% CI; 44.62% to 76.64%) and specificity was 57.45 % (95% CI; 42.18% to 71.74%).

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