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Foot-and-mouth disease outbreaks investigations at Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013

Research Paper | November 1, 2017

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Umer Farooq, Khalid Naeem, Aamer Bin Zahur, Muhammad Azeem Khan, Umer Sidique

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Int. J. Biosci.11( 5), 346-356, November 2017

DOI: http://dx.doi.org/10.12692/ijb/11.5.346-356


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This study reports characterization of Foot-and-Mouth Disease Virus (FMDV) from clinical suspected cases collected from Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013. The attempts were made for the recovery FMDV in LFBK cell line and the viruses were confirmed by real time PCR and serotyped by indirect sandwich ELISA. Overall ELISA confirmed 51.2% FMD outbreaks. A total of 39 viruses (45.4%) were isolated from outbreaks which include sero-type O (35.9%), A (28.2%) and Asia-1 (35.9%). FMDV sero-type O isolates were characterized within the PanAsia2 and the sub lineage identified was PanAsia2 ANT-10 & PanAsia2 BAL-09. FMDV sero-type A was grouped within the lineage A/Asia/Iran-05. The sub lineage identified within FMDV Iran-05 was HER-10, FAR-11 & ESF-10. FMDV sero-type Asia-1 was classified in group VII (Sindh-08). The diagnostic sensitivity of ELISA was found to be 61.54% (95% CI; 44.62% to 76.64%) and specificity was 57.45 % (95% CI; 42.18% to 71.74%).


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Foot-and-mouth disease outbreaks investigations at Sindh, Punjab and Islamabad, Pakistan during the year 2012-2013

Abubakar M, Khan EUH, Arshed MJ, Gonzales J, Ferrari G, Hussain M, Ali Q. 2015. An appraisal on the occurrence of Foot-and-mouth disease virus sero-types in cattle and buffaloes, Pakistan. Archives Virology 160(6), 1561-4.

Ahmad R, Iqbal J, Akbar G. 2002. An outbreak of Foot-and-Mouth Disease in a herd of crossbred cattle. Pakistan Veterinary Journal 22, 201–203.

Alexandersen S, Mowat N. 2005. Foot-and-mouth disease: host range and pathogenesis. Current Topics in Microbiology and Immunology 288, 9-42.

Alexandersen S, Quan M, Murphy C, Knight J, Zhang Z. 2003b. Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with Foot-and-Mouth Disease Virus. Journal of Comparative Pathology 129, 268–282.

Alexandersen S, Zhang Z, Reid SM, Hutchings GH, Donaldson AI. 2002. Quantities of infectious virus and viral RNA recovered from sheep and cattle experimentally infected with Foot-and-Mouth Disease Virus O UK 2001. Journal of General Virology 83, 1915-1923.

Anjum R, Hussain M, Zahoor AB, Irshad H, Farooq U. 2006. Epidemiological analyses of Foot-and-Mouth Disease in Pakistan. International Journal of Agriculture and Biology 5, 648–651.

Arzt J, Baxt B, Grubman MJ, Jackson T, Juleff N, Rhyan J, Rieder E, Waters R, Rodriguez LL. 2011. The pathogenesis of Foot-and-Mouth Disease II: viral pathways in swine, small ruminants, and wildlife; myotropism, chronic syndromes, and molecular virus-host interactions. Transboundary and Emerging Diseases 58, 305-326.

Arzt J, Juleff N, Zhang Z, Rodriguez LL. 2011. The pathogenesis of Foot-and-Mouth Disease I: viral pathways in cattle. Transboundary and Emerging Diseases 58, 291-304.

Belsham GJ. 1993. Distinctive features of Foot-and-Mouth Disease Virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. Progress in Biophysics and Molecular Biology 60 (3), 241-260.

Brito B, Pauszek SJ, Eschbaumer M, Stenfeldt C, de Carvalho Ferreira HC, Vu LT, Phuong NT, Hoang BH, Tho ND, Dong PV, Minh PQ, Long NT, King DP, Knowles NJ, Dung DH, Rodriguez LL, Arzt J. 2017a. Phylodynamics of Foot-and-Mouth Disease Virus O/Pan Asia in Vietnam 2010–2014. Veterinary Research 48, 24.

Brooksby JB, Rogers J. 1957. Methods used in typing the virus of Foot-and-Mouth Disease at Pirbright, 1950-55. In: Methods of typing and cultivation of Foot-and-Mouth Disease virus, project No. 208. Paris: European Productivity Agency of the Organization of European Cooperation (OEEC). p. 31-34.

Callahan JD, Brown F, Osorio FA, Sur JH, Kramer E, Long GW, Lubroth J, Ellis SJ, Shoulars KS, Gaffney KL, Rock DL, Nelson WM. 2002. Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of Foot-and-Mouth Disease Virus. Journal of the American Veterinary Medical Association 220, 1636-1642.

Carrillo C, Tulman ER, Delhon G, Lu Z, Carreno A, Vagnozzi A, Kutish GF, Rock DL. 2005. Comparative genomics of Foot-and-Mouth Disease Virus. Journal of Virology 79(10), 6487-6504.

de Carvalho Ferreira HC, Pauszek SJ, Ludi A, Huston CL, Pacheco JM, Le VT, Nguyen PT, Bui HH, Nguyen TD, Nguyen T, Nguyen TT, Ngo LT, Do DH, Rodriguez L, Arzt J. 2017. An integrative analysis of Foot-and-Mouth Disease Virus carriers in Vietnam achieved through targeted surveillance and molecular epidemiology. Trans boundary and Emerging Diseases 64, 547-563.

Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792-1797.

Ferris NP, Dawson M. 1988. Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of Foot-and-Mouth and Swine Vesicular Diseases. Veterinary Microbiology 16 (3), 201–209.

Firoozi Bandpay MR, Amighi M, Mastan MB, Maleknejad P. 1974. The outbreak of Foot-and-Mouth Disease (Asia-1 type) in 1973 in Iran. Bulletin de l’office International des Épizooties 81, 681-687.

Jamal SM, Ahmed S, Hussain M, Ali Q. 2010. Status of Foot-and-Mouth Disease in Pakistan. Archives of Virology 155, 1487-1491.

Jamal SM, Ferrari G, Ahmed S, Normann P, Belsham GJ. 2011. Genetic diversity of foot-and-Mouth disease virus serotype O in Pakistan and Afghanistan 1997–2009. Infection, Genetics and Evolution 11(6), 1229–1238.

Klein J, Hussain M, Ahmad M, Afzal M, Alexandersen S. 2008. Epidemiology of Foot-and-Mouth Disease in Landhi Dairy Colony, Pakistan, the world largest Buffalo colony. Virology Journal 5, 53-68.

Knowles NJ, Wadsworth J, Bachanek-Bankowska K, King DP. 2016. VP1 sequencing protocol for Foot-and-Mouth Disease Virus molecular epidemiology. Revue Scientifique et Technique de l’office International des Épizooties 35, 741-755.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33, 1870-1874.

LaRocco M, Krug PW, Kramer E, Ahmed Z, Pacheco JM, Duque H, Baxt B, Rodriguez LL. 2013. A continuous bovine kidney cell line constitutively expressing bovine αVβ6 integrin has increased susceptibility to Foot-and-Mouth Disease Virus. Journal of Clinical Microbiology 51, 1714-1720.

Longjam N, Sarmah AK, Deb R, Mathapati B, Saxena VK, Tayo T. 2011. Detection of Foot-and-Mouth Disease Virus sero-type O by sandwich ELISA, viral isolation and multiplex PCR. Online Journal of Veterinary Research, 15(1), 76–92.

Ludi A, Ahmed Z, Pomeroy LW, Pauszek SJ, Smoliga GR, Moritz M, Dickmu S, Abdoulkadiri S, Arzt J, Garabed R, Rodriguez LL. 2016. Sero-type Diversity of Foot-and-Mouth-Disease Virus in Livestock without History of Vaccination in the Far North Region of Cameroon. Transboundary and Emerging Diseases 63, e27-e38.

Morzaria S, Oberoi M, Ahuja V. 2012. Virus Pool 2 – South Asia. In: Proceedings of the FAO/OIE Global Conference on Foot and Mouth Disease Control, Bangkok, 27-29 June 2012, Thailand. 141.

Murphy ML, Forsyth MA, Belsham GJ, Salt JS. 1999. Localization of Foot-and-Mouth Disease Virus RNA by in situ hybridization within bovine tissues. Virus Research 62, 67-76.

Pauszek SJ, Barrera JDC, Goldberg T, Allende R, Rodriguez LL. 2011. Genetic and antigenic relationships of vesicular stomatitis viruses from South America. Archives of Virology 156, 1961.

Roeder PL, Le Blanc Smith PM. 1987. Detection and typing of Foot-and-Mouth Disease Virus by enzyme-linked immunosorbent assay: a sensitive, rapid and reliable technique for primary diagnosis. Research In Veterinary Science 43, 225–232.

Saeed A, Khan QM, Waheed U, Arshad M, Asif M, Farooq M. 2011. RT-PCR evaluation for identification and sequence analysis of Foot-and-Mouth Disease sero-type O from 2006 to 2007 in Punjab, Pakistan. Comparative Immunology, Microbiology and Infectious Diseases 34, 95-101.

Stenfeldt C, Eschbaumer M, Rekant SI, Pacheco JM, Smoliga GR, Hartwig EJ, Rodriguez LL, Arzt J. 2016. The Foot-and-Mouth Disease Carrier State Divergence in Cattle. Journal of Virology 90(14), 6344-64.

Sumption KJ, McLaws M, Bartels CJ, De Leeuw PW, Domenech J, Lubroth J, Ferrari G. 2012. The Progressive Control Pathway for FMD (PCP-FMD): a tool for developing sustainable long-term national and regional FMD control. In: Proceedings of the FAO/OIE Global Conference on Foot and Mouth Disease Control, Bangkok, 27-29 June 2012, Thailand. 30.

Thrusfield M. 2005. Veterinary Epidemiology, 3rd Ed. Publisher: Blackwell Publishing. Oxford University Press, UK.

Valarcher JF, Knowles NJ, Saraiva V. 2008. Epidemiological patterns of Foot-and-Mouth Disease worldwide. Transboundary and Emerging Diseases 55, 57-72.

Yasin SA, Huq MM. 1960. Foot-and-mouth disease in Pakistan. Bulletin de l’office International des Épizooties 54, 378–388.

Zahur AB, Irshad H, Hussain M, Anjum R, Khan MQ. 2006. Transboundary animal diseases in Pakistan. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health journal 53, 19-22.

Zhang ZD, Kitching RP. 2001. The localization of persistent Foot-and-Mouth Disease Virus in the epithelial cells of the soft palate and pharynx. Journal of Comparative Pathology 124 (2-3), 89–94.