Genes involved in the regulation of starch metabolism and defense response determine resistance in citrus against huanglongbing

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Research Paper 01/08/2020
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Genes involved in the regulation of starch metabolism and defense response determine resistance in citrus against huanglongbing

Rozina Aslam, Iqrar Ahmad Khan, Muhammad Sarwar Yaqub
Int. J. Biosci. 17(2), 121-129, August 2020.
Copyright Statement: Copyright 2020; The Author(s).
License: CC BY-NC 4.0

Abstract

Different diseases and pests affect the genus citrus, resulting in reduced quality and taste of fresh fruit, low production and ultimately less profit for the growers. Huanglongbing (HLB) is the major problem for citrus-producing countries including Pakistan all over the world. Asian citrus psyllid (ACP) was collected from HLB positive sweet orange cv. succari (Citrus sinensis (L.) Osbeck) field trees and released on healthy plants of succari in the growth room under controlled conditions for the infestation to obtain bud/grafts for inoculation in experimental mandarin plants. SYBR green-based real-time qPCR was performed to differentiate the expression of carbohydrate metabolism and defense response-related selected genes in HLB infected and healthy leaf samples of kinnow, citrus sunki, parsons special and sun chu sha. Gene expression data analysis results represented that glucose-1-phosphate adenyl transferase, starch synthase and cytochrome P450 monooxygenase 83B1 genes may play a role in the tolerance against HLB.

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