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Genetic divergence among Egyptian populations of Drosophila melanogaster and Canton-S wild type strain

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Research Paper 01/07/2015
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Genetic divergence among Egyptian populations of Drosophila melanogaster and Canton-S wild type strain

Ismael A. Khatab, Antar N. El-Banna, Amira S. El-Keredy
J. Bio. Env. Sci.7( 1), 173-179, July 2015.
Certificate: JBES 2015 [Generate Certificate]

Abstract

Genetic divergence and interrelations of four geographic populations of Drosophila melanogaster (three populations from Egypt namely; Tanta, Kafr-Elshiekh and Wady El-Natron areas and Canton-S wild type strain) were analyzed by PCR Random Amplified Polymorphic DNA (PCR-RAPD). Out of twenty random primers used only 16 primers efficiently produced 79 RAPD bands, of which 42 were polymorphic. The dendrogram constructed using UPGMA based on the RAPD data showed that there was similarity among the populations collected from Egypt and do not show clear diversity or population differentiation, which refers to population growth. Moreover, used RAPD primers in this study classified the three populations from Egypt into two clusters according to their geographical origin, indicating recent population differentiation. In contrast, population Canton-S (wild type strain) was highly diverged and located in separated cluster. These results agreed with conclusions of the previous studies that Drosophila originated in Africa and colonized the rest of the world only recently. Moreover, D. melanogaster was highly differentiated.

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