Genetic divergence among Egyptian populations of Drosophila melanogaster and Canton-S wild type strain
Paper Details
Genetic divergence among Egyptian populations of Drosophila melanogaster and Canton-S wild type strain
Abstract
Genetic divergence and interrelations of four geographic populations of Drosophila melanogaster (three populations from Egypt namely; Tanta, Kafr-Elshiekh and Wady El-Natron areas and Canton-S wild type strain) were analyzed by PCR Random Amplified Polymorphic DNA (PCR-RAPD). Out of twenty random primers used only 16 primers efficiently produced 79 RAPD bands, of which 42 were polymorphic. The dendrogram constructed using UPGMA based on the RAPD data showed that there was similarity among the populations collected from Egypt and do not show clear diversity or population differentiation, which refers to population growth. Moreover, used RAPD primers in this study classified the three populations from Egypt into two clusters according to their geographical origin, indicating recent population differentiation. In contrast, population Canton-S (wild type strain) was highly diverged and located in separated cluster. These results agreed with conclusions of the previous studies that Drosophila originated in Africa and colonized the rest of the world only recently. Moreover, D. melanogaster was highly differentiated.
Baudry E, Viginier B, Veuille M. 2004. Non-African populations of Drosophila melanogaster have a unique origin. Molecular Biology and Evolution 21, 1482–1491.
Begun D, Aquadro CF. 1993. African and North American populations of Drosophila melanogaster are very different at the DNA level. Nature 365, 548– 550.
David JR, Capy P. 1988. Genetic variation of Drosophila melanogaster natural populations. Trends Genetics 4, 106–111.
Denver DR, Morris JT, Streelman SK, Kim M, Lynch M, Thomas WK. 2005. The transcriptional consequences of mutation and natural selection in Caenorhabditis elegans. Nature Genetics 37, 544-548.
Gilchrist GW, Huey RB, Serra L. 2000. Rapid evolution of wing size clines in Drosophila subobscura. Genetica 112-113, 273-86.
Houle D, Mezey JG, Galpern P, Carter A. 2003. Automated measurement of Drosophila wings. BMC Evolutionary Biology 3, 25.
Huey RB, Gilchrist GW, Carlson ML, Berrigan D, Serra L. 2000. Rapidevolution of a geographic cline in size in an introduced fly. Science 287, 308-309.
James W, Curtsinger HF, Amy SR, Kristi K, Khazaeli A. 1998. Genetic analysis of extended life span in Drosophila melanogaster I. RAPD screen for genetic divergence between selected and control lines. Genetica 104, 21–32.
Kennington WJ, Killeen JR, Goldstein DB, Partridge L. 2003. Rapid laboratory evolution of adult wing area in Drosophila melanogaster in response to humidity. Evolution 57, 932–936.
Langley CH, Stevens K, Cardeno C, Lee YC, Schrider DR, Pool JE, Langley SA, Suarez C, Corbett-Detig RB, Kolaczkowski B, Fang S, Nista PM, Holloway AK, Kern AD, Dewey CN, Song YS, Hahn MW, Begun DJ. 2012. Genomic variation in natural populations of Drosophila melanogaster. Genetics 92, 533-98.
Mackay TFC, Richards S, Stone EA, Barbadilla A, Ayroles JF. 2012. The Drosophila melanogaster genetic reference panel. Nature 482, 173–178.
Megeed MS. 1982. Genetical studies on Drosophila melanogaster. Genetic differentiation between some Egyptian populations of Drosophila melanogaster. M.Sc. Thesis, Fac. Agric., Kafr El-Sheikh, Tanta Univ., Egypt.
Pascual M, Charles FA, Vanessa S, Serra L. 2001. Microsatellite Variation in Colonizing and Palearctic Populations of Drosophila subobscura, Molecular Biology and Evolution 18, 731–740.
Pascual M, Schug MD, Aquadro CF. 2000. High density of long dinucleotide microsatellites in D. subobscura. Molecular Biology and Evolution 17, 1259–1267.
Penelope R, Thornton R, Charlesworth B, Andolfat P. 2005. Multilocus patterns of nucleotide variability and the demographic and selection history of Drosophila melanogaster populations. Genome Research 15, 790-799.
Pool JE, Corbett-Detig RB, Sugino RP, Stevens KA, Cardeno CM. 2012. Population Genomics of Sub-Saharan Drosophila melanogaster: African Diversity and Non-African Admixture. PLoS Genetics 8, e1003080. http://dx.doi.org/10.1371/journal.pgen.1003080.
Rifkin SA, Houle D, Kim J, White KP. 2005. A mutation accumulation assay reveals a broad capacity for rapid evolution of gene expression. Nature 438, 220-223.
Sharma AK, Sharma A. 1999. Plant chromosomes analysis mainpulation and engineering, ISBN, 5702-387-3.
Thongatabam B, Ramachandra NB. 2010. Genetic Variability Assessed by Competitive Ability and ISSR Markers in the Members of the Nasuta-albomicans Complex of Drosophila. Nature and Science 8, 29-42.
Weber K, Johnson N, Champlin D, Patty A. 2005. Many P-element insertions affect wing shape in Drosophila melanogaster. Genetics 169, 1461-1475.
Yutaka I, Watada M. 2006. A new incipient polymorphic inversion, In(2R)O, in D. melanogaster Japanese populations. Drosophila Information Service 89, 77-81.
Ismael A. Khatab, Antar N. El-Banna, Amira S. El-Keredy (2015), Genetic divergence among Egyptian populations of Drosophila melanogaster and Canton-S wild type strain; JBES, V7, N1, July, P173-179
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