Genetic diversity in some euphrates poplar (Populus euphratica O.) ecotypes in Iran using microsatellites (SSRs) markers

Paper Details

Research Paper 01/07/2016
Views (679)
current_issue_feature_image
publication_file

Genetic diversity in some euphrates poplar (Populus euphratica O.) ecotypes in Iran using microsatellites (SSRs) markers

Hossein Tavakoli-Neko, Anoushirvan Shirvany, Mohammad Hassan Assareh, Mohammad Reza Naghavi,Mohammad Pessarakli
J. Biodiv. & Environ. Sci. 9(1), 434-440, July 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Euphrates poplar (Populus euphratica Oliv.) is a unique woody species which is naturally distributed in desert areas of some parts of Asia and Africa. Because of its outstanding features, it is a model plant to study environment stress tolerance. This research was conducted to evaluate the genetic variation in 12 ecotypes of P. euphratica in Iran through 10 simple sequence repeats (SSRs) primers from 2015 to 2016. The average numbers of alleles observed in each ecotype was 6.43 and average numbers of effective alleles was 5.58. The average of observed heterozygosis was 0.65 and average of expected heterozygosis was 0.80. The ecotypes were complying with Hardy-Weinberg’s equilibrium in all loci, except Marand ecotype for two of the ten primers that showed deviation of the balance (p<0.05). The Shannon information index was 1.75. Analysis of molecular variance (AMOVA) showed that 3% of molecular variance belongs to intra-population and 97% belongs to inter-population. The PCA showed six principal components covered 22.86% of the total variance. Clustering analysis of ecotypes through genetic distance, the examined ecotypes were divided into six groups, while the geographic distance did not have any significant effect on genetic differences. Overall, the results indicated that P. euphratica stands covered a vast area of Iran in the past, and probably had not been fragmented; it seems vast areas of Iran are potentially ready for revival of P. euphratica forests.

FAO. 1979. Poplar and Willow in wood production and land use 328 p.

Lin SZ, Zhang ZY, Zhang Q, Lin YZ. 2006. Progress in the study of molecular genetic improvements of poplar in China. Journal of Integrative Plant Biology 48(9), 1001-1007.

Murray MG, Thompson WF. 1980. Rapid isolation of high molecular weight plant. Nucleic Acids Research 8, 4321-4325.

Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceeding of the National Academy of Sciences of the USA 76, 5269-5273.

Pascal E, Steffen F, Martin S. 2009. Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica. Jour-nal of Forestry Research 20(3), 195-198.

Sabeti H. 2003. Forests, Trees and Shrubs of Iran. 3 edn. University of Yazd Yazd, Iran.

Saito Y, Shiraishi S, Tanimoto T, Yin L, Watanabe S, Ide Y. 2002 Genetic diversity of Populus euphratica populations in northwestern China determined by RAPD DNA. New Forests 23, 97-103.

Tuskan GA, DiFazio SP, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, et al. 2006. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313(5793), 1596-1604.

Wang J, Li Z, Guo Q. 2011. Genetic variation within and among populations of a desert poplar (Populus euphratica) revealed by SSR markers. Annals of forest science 68, (1143-1149).

Wang J, Wu Y, Ren G, Guo Q, Liu J. 2011. Genetic Differentiation and Delimitation among Ecologically Diverged Populus euphratica and P.pruinosa. . PLoS ONE 6(10), e26530.

Wu Y, wamg W, Liu J. 2008. Development and characterization of microsatellite markers in Populus euphratica. Molecular Ecology Resources 8, 1142-1144.

Xu F, Feng, S, Wu, R, Du FK. 2013. Two highly validated SSR multiplexes (8-plex) for Euphrates’ poplar, Populus euphratica (Salicaceae). Molecular Ecology Resources 13, 144-153.

Related Articles

Agroforestry in woody-encroached Sub-Saharan savannas: Transforming ecological challenges into sustainable opportunities

Yao Anicet Gervais Kouamé, Pabo Quévin Oula, Kouamé Fulgence Koffi, Ollo Sib, Adama Bakayoko, Karidia Traoré, J. Biodiv. & Environ. Sci. 27(3), 10-22, September 2025.

Extreme rainfall variability and trends in the district of Ouedeme, municipality of Glazoue (Benin)

Koumassi Dègla Hervé, J. Biodiv. & Environ. Sci. 27(3), 1-9, September 2025.

Heterosis breeding, general and specific combining ability and stability studies in pearl millet: Current trends

Ram Avtar, Krishan Pal, Kavita Rani, Rohit Kumar Tiwari, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 117-124, August 2025.

Combining ability, heterosis and stability for yield and fibre quality traits in cotton: Breeding approaches and future prospects

Rohit Kumar Tiwari, Krishan Pal, R. P. Saharan, Ram Avtar, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 109-116, August 2025.

Bridging the COPD awareness gap in marginalized populations: Findings from a multicentre study in Khalilabad, Sant Kabir Nagar, Uttar Pradesh, India

Anupam Pati Tripathi, Jigyasa Pandey, Sakshi Singh, Smita Pathak, Dinesh Chaudhary, Alfiya Mashii, Farheen Fatima, J. Biodiv. & Environ. Sci. 27(2), 97-108, August 2025.

Antioxidant and anti-inflammatory activity of Pleurotus citrinopileatus Singer and Pleurotus sajor-caju (Fr.) Singer

P. Maheswari, P. Madhanraj, V. Ambikapathy, P. Prakash, A. Panneerselvam, J. Biodiv. & Environ. Sci. 27(2), 90-96, August 2025.

Mangrove abundance, diversity, and productivity in effluent-rich estuarine portion of Butuanon River, Mandaue City, Cebu

John Michael B. Genterolizo, Miguelito A. Ruelan, Laarlyn N. Abalos, Kathleen Kay M. Buendia, J. Biodiv. & Environ. Sci. 27(2), 77-89, August 2025.

Cytogenetic and pathological investigations in maize × teosinte hybrids: Chromosome behaviour, spore identification, and inheritance of maydis leaf blight resistance

Krishan Pal, Ravi Kishan Soni, Devraj, Rohit Kumar Tiwari, Ram Avtar, J. Biodiv. & Environ. Sci. 27(2), 70-76, August 2025.