Genetic variation of Agropyron Trichophorum accessions using ISSR molecular marker
Paper Details
Genetic variation of Agropyron Trichophorum accessions using ISSR molecular marker
Abstract
Genetic variation for 24 accessions of Agopyron trichophorm evaluated using 13 ISSR primers. In total 90 bands (loci) were obtained out of which 88 bands were polymorph in accessions studied. The primers of IS14, UBC808, UBC825, UBC835, UBC842 and UBC886 considering polymorphism information content (PIC) were useful for polymorphism study in Agropyron genus in the future researches. The similarity between accessions based on Dice’s coefficient was low; therefore there was a high genetic variation among accessions. The three groups of accessions obtained by the results of cluster analysis and principal coordinate analysis (PCo) which these results confirmed by molecular variance analysis. Grouping of accessions indicated that genetic variations do not in agreement with the geographical distribution of accessions. Finally, the accessions of the first group [Shahrekord (1), Semirom (14), Semirom (10), Mazandaran (23), Eghlid (19), Eghlid (20), Tehran (21), Esfahan (22) and Gorgan (24)] had the highest genetic distance with the accessions of the third group [Karaj (6), Bijar (7), Salmas (13), Semirom (15) and Yasouj (18)], based on ISSR markers. Therefore, considering the genetic distance between these groups, using of first and third group accessions can be useful in breeding programs, to utilization of heterosis.
Anderson JA, Church JE, Autrique SD, Thanksley S, Sorrells ME. 1993. Optimizing parental selection for genetic linkage map. Genome 36, 181-188.
Arghavani A, Asghari A, Shokrpour M, Mohammaddost C. 2010. Genetic Diversity in Ecotypes of Two Agropyron Species using RAPD Markers. Research Journal of Environmental Sciences 4, 50-56.
Behura SK. 2006. Molecular marker systems in insects: Current trends and future avenues. Molecular Ecology 15, 3087–3113.
Bor NL. 1970. Gramineae. In: Flora Iranica. Rechinger, K.H. (Ed.). Akademische Druk-u. Verlagsanstalt, Graz, Austria 70, 571-573.
Che YH, Li LH. 2007. Genetic diversity of prolamines in Agropyron mongolicum Keng indigenous to northern China. Genetic Resources and Crop Evolution 54, 1145–1151. http://dx.doi.org/10.1007/s10722-006-9006-7
Che YH, Yang YP, Yang XM, Li XQ, Li LH. 2011. Genetic diversity between ex situ and in situ samples of Agropyron cristatum (L.) Gaertn. Based on simple sequence repeat molecular markers. Crop and Pasture Science 62(8), 639-644. http://dx.doi.org/10.1071/CP11065
De La Rosa R, James C, Tobutt KR. 2002. Isolation and characterization of polymorphic microsatellite in olive (Olea europaea L.) and their transferability to other genera in Oleaceae. Molecular Ecology Notes 2, 265-267.
Dice LR. 1945. Measures of the amount of ecologic association between species. Ecology 26(3), 297-302. http://dx.doi.org/10.2307/1932409
Dizkirici A, Kaya Z, Cabi E, Dogan M. 2010. Phylogenetic relationships of Elymus L. and related genera (Poaceae) based on the nuclear ribosomal internal transcribed spacer sequences. Turkish journal of botany 34, 467-478.
Fasih Z, Farshadfar M, Safari H. 2013. Genetic diversity evaluation of within and between populations for Festuca arundinacea by ISSR markers. International Journal of Agriculture and Crop Sciences 5(10), 1468-1472
Hu T, Li H, Li DSJ, Fu J. 2011. Assessing genetic diversity of perennial ryegrass (Lolium perenne L.) from four continents by inter-simple sequence repeat (ISSR) markers. African Journal of Biotechnology 10(83), 19365-19374.
Ma X, Zhang XQ, Zhou YH, Bai SQ, Liu W. 2008. Assessing genetic diversity of Elymus sibiricus (Poaceae: Triticeae) populations from Qinghai-Tibet plateau by ISSR markers. Biochemical Systematics and Ecology 36, 514–522.
Murry MG, Tompson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321-4325.
Pivoriene O, Pasakinskiene I. 2008. Genetic diversity assessmentin perennial Ryegrass and Festulolium by ISSR fingrprinting. Agriculture 95(2), 125-133.
Refoufi A, Esnault MA. 2008. Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data. Biologia Plantarum 52(3), 543-547. http://dx.doi.org/10.1007/s10535-008-0106-4
Rouf Mian MA, Andrew AH, John CZ. 2002. Determination of Genetic Diversity in Tall Fescue with AFLP Markers. Crop Science 42, 944-950.
Sicard D, Nanni L, Porfiri O, Bulfon D, Papa R. 2005. Genetic diversity of Phaseolus vulgaris L. and P. coccineus L. landraces in central Italy. Plant Breeding 124(5), 464–472. http://dx.doi.org/10.1111/j.1439-0523.2005.01137.x
Souframanien J, Gopalkrishna T. 2004. A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theoretical and Applied Genetics 109, 1687-1693. http://dx.doi.org/10.1007/s00122-004-1797-3
Sun G L, Diaz O, Salomon B, von Bothmer R. 1999. Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers. Genome 42, 420-431.
Sun G L, Salomon B, von Bothmer R. 2002. Microsatelllite polymorphism and genetic differentiation in three Norwegian populations of Elymus alaskanus (Poaceae). Plant Systematics and Evolution 234, 101–110. http://dx.doi.org/10.1007/s00606-002-0211-3
Surendhar RCh, Parsad BA, Mallikarjuna SB P, kaladhar K, Sarla N. 2009. ISSR markers based on GA and AG repeats reveal genetic relationship among rice varieties tolerant to drought, flood, or salinity. Journal of Zhejiang University SCIENCE B 10(2), 133–141. http://dx.doi.org/10.1631/jzus.B0820183
Szczepaniak M, Bieniek W, Boroń P, Szklarczyk M, Mizianty M. 2009. A contribution to characterization of genetic variation in some natural Polish populations of Elymus repens (L.) Gould and Elymus hispidus (Opiz) Melderis (Poaceae) as revealed by RAPD markers. Plant Biology 11(5), 766–773. http://dx.doi.org/10.1111/j.1438-8677.2008.00171.x.
Wolfe AD, Xiang QY, Kephart SR. 1998. Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hyper variable inter simple sequence repeat markers. Molecular Ecology 7, 1107–1125.
Xu GH, Su WY, Shu YJ, Cong WW, Wu L, Guo CH. 2012. RAPD and ISSR-assisted identification and development of three new SCAR markers specific for the Thinopyrum elongatum E (Poaceae) genome. Genetics and Molecular Research 11(2), 1741-1751. http://dx.doi.org/10.4238/2012.June.29.7.
Yang RW, Tsujimoto H, Ding CB, Zhang L, Wang XL, Zhou YH. 2011. Phylogenetic relationships among Hystrix species and related species based on expressed sequence tag-polymerase chain reaction. Journal of Systematics and Evolution 49(1), 65–71. http://dx.doi.org/10.1111/j.1759-6831.2010.00107.x
Zietkiewicz E, Rafalski A, Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176–183.
Hooman Shirvani, Ali Reza Etminan, Hooshmand Safari, 2014. Genetic variation of Agropyron Trichophorum accessions using ISSR molecular marker. J. Biodiv. Environ. Sci., 5(4), 23-30.
Copyright © 2014 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.