J. Bio. Env. Sci.5(4), 593-600, October 2014
Twenty-one turmeric accessions collected from seven provinces in Mindanao were grown in a uniform environment and analyzed for curcumin content. Rhizome bits of each accession were planted in big plastic bags and randomly laid out in the field. Rhizome samples harvested from each accession were analyzed for curcumin content using High Performance Liquid Chromatography (HPLC) on a Novapak C18 column with the solvent system consisting of methanol:acetonitrile (80:20) and detection at 370 nm. Highly significant differences in curcumin content were observed among the turmeric accessions, varying from as low as 1.2 mg/g for Accession 3 to as high as 102.4 mg/g for Accession 20. These results reveal the existence of genetic variation in the turmeric accessions collected from the different provinces in Mindanao with high curcumin yielding accessions identified which could be recommended for cultivation.
Garg SN, Bansal RP, Gupta MM, Kumar S. 1999. Variation in the rhizome essential oil and curcumin contents and oil quality in the land races of turmeric Curcuma longa of North Indian plains. Flavour and Fragrance Journal 14, 315-318.
Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. 2002. Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin. Journal of Agricultural and Food Chemistry 50, 3668-3672.
Joe B, Vijaykumar M, Lokesh BR. 2004. Biological properties of curcumin-cellular and molecular mechanisms of action. Critical Reviews in Food Science and Nutrition 44, 97-111.
Katsuyama Y, Kita T, Funa N, Horinouchi S. 2009a. Curcuminoid biosynthesis by two type III polyketides synthases in the herb Curcuma longa. Journal of Biological Chemistry 284, 11160-11170.
Katsuyama Y, Kita T, Horinouchi S. 2009b. Identification and characterization of multiple curcumin synthases from the herb Curcuma longa. FEBS Letters 583, 2799-2803.
Kulkarni SJ, Maske KN, Budre MP, Mahajan RP. 2012. Extraction and purification of curcuminoids from turmeric (Curcuma longa L.) International Journal of Pharmacology and Pharmaceutical Technology 1, 81-84.
Leong-Ŝkornickova J, Ŝida O, Jarolimova V, Sabu M, Fer T, Travnicek P, Suda J. 2007. Chromosome numbers and genome size variation in Indian species of Curcuma (Zingiberaceae). Annals of Botany 100, 505-526.
Nair RR, Shiva KN, Anchu S, Zachariah TJ. 2010. Characterization of open-pollinated seedling progenies of turmeric (Curcuma longa L.) based on chromosome number, plant morphology, rhizome yield and rhizome quality. Cytologia 75, 443-449.
Nayak S, Naik PK, Acharya LK, Pattnaik AK. 2006. Detection and evaluation of genetic variation in 17 promising cultivars of turmeric (Curcuma longa L.) using 4C nuclear DNA content and RAPD markers. Cytologia 71, 49-55.
Pandey A, Katiyar SK. 2010. Determination and comparison of the curcuminoid pigments in turmeric genotypes (Curcuma domestica Val) by high-performance liquid chromatography. International Journal of Pharmacology and Pharmaceutical Sciences 2, 125-127.
Pathak N, Naithani V, Singh J, Bhole P, Chaudhary M. 2010. An assessment of variation in active ingredients of Ampucare from different zones of India. International Journal of Pharmaceutical Science and Drug Research 2, 123-126.
Sasikumar B. 2005. Genetic resources of Curcuma: diversity, characterization and utilization. Plant Genetic Resources: Characterization and Utilization 3, 230-251.
Wagner H, Bladt S. 1996. Plant drug analysis: a thin layer chromatography atlas. Berlin: Springer-Verlag, 384 p.