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Genus and species-specific PCR revealed the presence of beneficial lactic acid bacteria in traditional Bangladeshi fermented milk products Dahi

Md. Ahsanul Kabir, SM. Amanullah, Abdullah Al Noman, SM. Jahangir Hossain, Nasrin Sultana, Shahanaj Ferdousi Shejuty, Dipa Das

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Int. J. Biosci.21(6), 206-216, December 2022

DOI: http://dx.doi.org/10.12692/ijb/21.6.206-216

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Abstract

This study aimed to identify beneficial lactic acid bacteria (LAB) from traditional Bangladeshi fermented milk product dahi and provide efficient starter cultures. Twenty dahi samples were collected from four districts in Bangladesh and selected isolates were then subjected to biochemical tests including catalase and gas production tests and physiological tests (growth under different conditions). Finally, LAB was identified at the genus and species level using PCR. Sixteen isolates were identified based on Gram-positive and catalase-negative characteristics. All of the isolates were homo-fermentative in nature. “Morphological and biochemical tests revealed that the majority of the isolates belonged to the genera Streptococcus (45.0%) and Lactobacillus (35.0% Physiological tests demonstrated that the most predominant Streptococci were Streptococcus thermophilus which exhibited profuse growth at 45°C and a pH of 4-5, but no growth at below 15°C and even in 2% NaCl. The other strains were Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus acidophilus which manifested luxuriant growth at 45°C, moderate growth at 25°C and 2% NaCl and no growth below 15°C. Furthermore, genus-specific PCR followed by species-specific PCR also verified the presence of these species as 25% of the 16 isolates were identified as S. thermophilus, 31.2% as L. delbrueckii ssp. bulgaricus and 12.5% as L. acidophilus with these methods. Positive PCR results demonstrated that among the isolated strains, 25% of LAB were S. thermophilus, 31.2% were L. bulgaricus and 12.5% were L. acidophilus. These identified bacteria are the most widely used LAB in many fermented milk products such as dahi, yogurt and yogurt-like products. Therefore, they can be used as starter cultures to increase the probiotic and organoleptic properties of dahi and other Bangladeshi fermented milk products.

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Genus and species-specific PCR revealed the presence of beneficial lactic acid bacteria in traditional Bangladeshi fermented milk products Dahi

Abdullah, Sawsan A, Maymouna MO. 2010. “Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk, White Cheese and Rob in Sudan.” Pakistan Journal of Nutrition 9 12, 1203-6. https://doi.org/10.3923/PJN.2010.1203.1206.

Adeniyi, Bolanle A, Adewale A, Funmilola AA. 2015. “Antibacterial Activities of Lactic Acid Bacteria Isolated from Cow Faeces against Potential Enteric Pathogens.” African Health Sciences 15(3), 888. https://doi.org/10.4314/AHS.V15I3.24.

Amanullah SM, Kabir,mm Rahman MA, Halder P, Hossain SMJ, Samad MA. 2020. “Isolation, Identification and Biochemical Characterization of Lactic Acid Bacteria from Selected Yogurt Samples.” Bangladesh Journal of Livestock Research, November 64-72. https://doi.org/10.3329 /BJLR.V27I1.55170.

Azadnia P, Khan Nazer AH. 2009. “Identification of Lactic Acid Bacteria Isolated from Traditional Drinking Yoghurt in Tribes of Fars Province.” Iranian Journal of Veterinary Research 10(3), 235-40.

Boudraa G, Mahmoud T, Philippe P, Rachida S. 1990. “Effect of Feeding Yogurt versus Milk in Children with Persistent Diarrhea.” Journal of Pediatric Gastroenterology and Nutrition 11(4), 509-12. https://doi.org/10.1097/00005176-199011000-11.

Burentegusi, Taku M, Shoji N, Yoshihiro N, Akihiro A. 2002. “Identification of Lactic Acid Bacteria Isolated from Fermented Mare’s Milk ‘Chigee’ in Inner Mongolia, China.” Nihon Chikusan Gakkaiho 73(3), 441-48. https://doi.org/10.2508/

Chen, Shanshan Z, Guangfei H, Haiyan Y, Huaixiang T, Guozhong Z. 2017. “Role of Lactic Acid Bacteria on the Yogurt Flavour: A Review.” 20, S316-30. https://doi.org/10.1080/10942912. 2017.

Dhameliya HA, Sureshkumar NM, Himanshu M, Chandni S. 2020. “Biochemical and Molecular Characterization of Lactic Acid Bacteria (LAB) Isolated from Fermented Pulses.” Iranian Journal of Science and Technology, Transaction A: Science 44(5), 1279-86. https://doi.org/10.1007/S40995.

Dubernet, Nathalie D, Micheline G. 2002. “A PCR-Based Method for Identification of Lactobacilli at the Genus Level.” FEMS Microbiology Letters 214(2), 271-75. https://doi.org/10.1111/J.1574-6968.

Freitas M. 2017. “The Benefits of Yogurt, Cultures, and Fermentation.” The Microbiota in Gastrointestinal Pathophysiology: Implications for Human Health, Prebiotics, Probiotics, and Dysbiosis, January 209-23. https://doi.org/10.1016/B978-0-12

Gänzle, Michael G, Follador R. 2012. “Metabolism of Oligosaccharides and Starch in Lactobacilli: A Review.” Frontiers in Microbiology 3, (SEP). https://doi.org/10.3389/FMICB.2012.00340.

Harun-Ur-Rashid M, Kaname T, Minoru U, Taku M. 2006. “Identification and Characterization of Dominant Lactic Acid Bacteria Isolated from Traditional Fermented Milk Dahi in Bangladesh.” World Journal of Microbiology and Biotechnology 23(1), 125-33. https://doi.org/10.1007/S11274-006.

Hayek SA, Rabin G, Sulaiman OA, Albert K. 2019. “Cultivation Media for Lactic Acid Bacteria Used in Dairy Products.” Journal of Dairy Research 86(4), 490-502. https://doi.org/10.1017/S00220299

Hayek SA, Salam AI. 2013. “Current Limitations and Challenges with Lactic Acid Bacteria: A Review.” Food and Nutrition Sciences 4(11), 73-87. https:// doi.org/10.4236/FNS.2013.411A010.

Hutkins, Robert W. 2006. Microbiology and Technology of Fermented Foods. Iowa, USA: Blackwell Publishing.

Islam SMR, Tanzina AY, Foysal MJ. 2021. “Insights into the Nutritional Properties and Microbiome Diversity in Sweet and Sour Yogurt Manufactured in Bangladesh.” Scientific Reports 11(1), 1-15. https://doi.org/10.1038/s41598-021.

Isolauri E, Sütas Y, Kankaanpää P, Arvilommi H, Salminen S. 2001. “Probiotics: Effects on Immunity.” The American Journal of Clinical Nutrition 73(2), 444s-450s. https://doi.org/10.1093 /AJCN/73.2.444S.

Erzhad MF, Reza A, Ismail YS, Yulvizar C, Mazhitov B. 2018. “Characterization of Lactic Acid Bacteria from Local Cow´s Milk Kefir.” IOP Conference Series: Earth and Environmental Science 130(1), 012019. https://doi.org/10.1088/1755-1315/ 130/1/012019.

Litopoulou-Tzanetaki E, Tzanetakis N. 2014. “FERMENTED MILKS | Range of Products.” Encyclopedia of Food Microbiology: Second Edition, January 884-94. https://doi.org/10.1016/B978-0-12.

Ljungh A, Wadstrom T. 2006. “Lactic Acid Bacteria as Probiotics.” In Current Issues in Intestinal Microbiology, 2nd ed 7, 73-89. https://pubmed.ncbi

Mas-De-Xaxars T, Garcia-Gil, Jesús L. 2009. “Detection and Identification of Unknown Streptococcal Populations in Clinical Samples.” 21(3-4), 233-40. https://doi.org/10.3109/08910600 9034

Mathara JM, Ulrich S, Phillip MK, Samuel KM, Wilhelm HH. 2004. “Isolation, Identification and Characterisation of the Dominant Microorganisms of Kule Naoto: The Maasai Traditional Fermented Milk in Kenya.” International Journal of Food Microbiology 94(3), 269-78. https://doi.org/10.1016/J.IJFOODMICRO.2004.01.

McOrist AL, Michelle J, Anthony RB. 2002. “A Comparison of Five Methods for Extraction of Bacterial DNA from Human Faecal Samples.” Journal of Microbiological Methods 50(2), 131-39.

Khalil MI, Anwar MN. 2016. “Isolation, Identification and Characterization of Lactic Acid Bacteria from Milk and Yoghurts | Open Access Journals.” Research & Reviews: Journal of Food and Dairy Technology 4(3), 17-26.

Mithun SVD, Sarang S. 2015. “Isolation and Identification of Lactobacilli from Raw Milk Samples Obtained from Aarey Milk Colony.” International Journal of Scientific and Research 5(4).

Østlie HM, Merete HH, Judith AN. 2003. “Growth and Metabolism of Selected Strains of Probiotic Bacteria in Milk.” International Journal of Food Microbiology 87(1-2), 17-27. https://doi.org/ 10.1016/ S0168-1605(03)00044-8.

Ren D, Jianwei Z, Shengjie G. 2018. “Antimicrobial Characteristics of Lactic Acid Bacteria Isolated from Homemade Fermented Foods.” BioMed Research International.

Ren Y, Wenjun L, Heping Z. 2015. “Identification of Coccoidal Bacteria in Traditional Fermented Milk Products from Mongolia, and the Fermentation Properties of the Predominant Species, Streptococcus Thermophilus.” Korean Journal for Food Science of Animal Resources 35(5), 683. https://doi.org /10.5851 /KOSFA.2015.35.5.683.

Schrader J. 2007. Microbial Flavour Production. Flavours and Fragrances: Chemistry, Bioprocessing and Sustainability. Springer Berlin Heidelberg.

Shangpliang HNJ, Sharmila S, Ranjita R, Jyoti PT. 2017. “Some Technological Properties of Lactic Acid Bacteria Isolated from Dahi and Datshi, Naturally Fermented Milk Products of Bhutan.” Frontiers in Microbiology 8, 116. https://doi.org/10.3389/FMICB.2017.00116/BIBTEX.

Stanley G. 2003. “CHEESES-Starter Cultures Employed in Cheese-Making.” In Encyclopedia of Food Sciences and Nutrition 1051-56. Academic Press. https://doi.org/10.1016/B0-12-227055-X.

Tabasco R, Paarup T, Janer C, Peláez C, Requena T. 2007. “Selective Enumeration and Identification of Mixed Cultures of Streptococcus Thermophilus, Lactobacillus Delbrueckii Subsp. Bulgaricus, L. Acidophilus, L. Paracasei Subsp. Paracasei and Bifidobacterium Lactis in Fermented Milk.” International Dairy Journal 17(9), 1107-14. https://doi.org/10.1016/J.IDAIRYJ.2007.01.010.

Taye Y, Tadesse D, Haben F, Mesfin M. 2021. “Isolation and Identification of Lactic Acid Bacteria from Cow Milk and Milk Products.” Scientific World Journal. https://doi.org/10.1155/2021/4697445.

Moreno J, Peinado R. 2012. The Transformation of Must into Wine. Enological Chemistry, January 157-82. https://doi.org/10.1016/B978-0-12-388438-1.00011-X.

Tilahun B, Anteneh T, Diriba M, Andualem B, Zewdu T, Gary W. 2018. “Isolation and Molecular Identification of Lactic Acid Bacteria Using 16s RRNA Genes from Fermented Teff (Eragrostis Tef (Zucc.)) Dough.” International Journal of Food Science. https://doi.org/10.1155/2018/8510620.

Vantsawa PA, Umar TM, Timothy BP. 2017. “Isolation and Identification of Lactic Acid Bacteria with Probiotic Potential from Fermented Cow Milk (Nono) in Unguwar Rimi Kaduna State Nigeria.” American Journal of Molecular Biology 7(2), 99-106. https://doi.org/10.4236/AJMB.2017.72008.

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