Growth response of Bradyrhizobium inoculated soybean grown under maize intercropping systems, and P and K fertilization

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Research Paper 01/03/2017
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Growth response of Bradyrhizobium inoculated soybean grown under maize intercropping systems, and P and K fertilization

Daniel Nyoki, Patrick A. Ndakidemi
Int. J. Biosci.10( 3), 323-334, March 2017.
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The field experiment was carried out for two consecutive years to assess the effects of cropping systems, Rhizobium inoculation supplemented with P and K on growth performance of soybean. The experiment was laid out in split-split plot design with the main plots comprised of Rhizobia inoculation (with and without). The sub plots comprised of three cropping systems and the sub-sub plots having seven fertilizer levels (kg ha-1): Control, 20, 40 K, 26, 52 P, 26 P + 20 K and 52 P + 40 K. The experiment was replicated thrice. The results indicated that both treatments influenced most of the growth parameters of soybean assessed. Over un-inoculated treatments, Rhizobia inoculation significantly improved the growth of all parameters of soybean in this study. Similarly, P and K fertilization improved the growth of soybean over the control. Most of the parameters performed supper in plots treated with Rhizobia inoculation supplied with 26, 52 P and 20 K + 26 P kg ha-1 levels of P and K. The positive interactive effects of cropping systems, Rhizobium inoculation and P and K supplementation was observed on different growth parameters assessed. The positive interaction indicated the importance of these inputs and treatments for improving growth of crops and ultimately final yield in the study area.


Bai B, Suri VK, Kumar A, Choudhary AK. 2016. Influence of Dual Inoculation of AM Fungi and Rhizobium on Growth Indices, Production Economics, and Nutrient Use Efficiencies in Garden Pea (Pisum sativum L.), Communications in Soil Science and Plant Analysis 47(8), 941-954.

Bambara S, Ndakidemi AP. 2009. Effects of Rhizobium inoculation, lime and molybdenum on photosynthesis and chlorophyll content of Phaseolus vulgaris L. African Journal of Microbiology Research 3(11), 791-798.

Bambara S, Ndakidemi PA. 2010. Phaseolus vulgaris response to Rhizobium inoculation, lime and molybdenum in selected low pH soil in Western Cape, South Africa. African Journal of Agricultural Research 5(14), 1804-1811.

Brady NC. 2002. Phosphorus and potassium. In: The nature and properties of soils. Prentice-Hall of India, Delhi. p. 352.

Doornbos RF, van Loon LC, Bakker PAHM. 2012. Impact of root exudates and plant defense signaling on bacterial communities in the rhizosphere. A review. Agronomy for Sustainable Development 32(1), 227-243. 1007/s13593-011-0028-y.

Dwivedi A, Dev I, Kumar V, Yadav RS, Yadav M, Gupta D, Singh A, Tomar SS. 2015. Potential Role of Maize-Legume Intercropping Systems to Improve Soil Fertility Status under Smallholder Farming Systems for Sustainable Agriculture in India. International Journal of Life Sciences Biotechnology and Pharma Research 4(3), 145-157.

Fageria NK, Ferreira EPB, Melo LC, Knupp AM. 2014. Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia, Communications in Soil Science and Plant Analysis 45(12), 1583-1604,

Hamd Alla WA, Shalaby EM, Dawood RA, Zohry AA. 2014. Effect of Cowpea (Vigna sinensis L.) with Maize (Zea mays L.) Intercropping on Yield and Its Components. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 8(11), 1258-1264.

Hirpa T. 2014. Effect of Intercrop Row Arrangement on Maize and Haricot Bean Productivity and the Residual Soil. Global Journal of Science Frontier Research: D Agriculture and Veterinary 14(4), 27-34.

Lissbrant S, Berg WK, Volenec J, Brouder S, Joern B, Cunningham S, Johnson K. 2009. Phosphorus and Potassium Fertilization of Alfalfa. Purdue Cooperative Extension Service Publication AY-331-W.

Lobell DB, Field CB. 2007. Global scale climate–crop yield relationships and the impacts of recent warming. Environmental Research Letters 2, 1-7.

Maluleke MH, Bediako AA, Ayisi KK. 2005. Influence of maize-lablab intercropping on Lepidopterous stem borer infestation in maize. Journal of Economic Entomology 98(2), 384-388.

Mateos-Aparicio I, Redondo-Cuenca A, Villanueva-Suárez MJ, Zapata-Revilla MA. 2008. Soybean, a promising health source. Nutrición Hospitalaria 23(4), 305-312. PMid:18604315.

Megawer EA, Sharaan AN, EL-Sherif AM. 2010. Effect of intercropping patterns on yield and its components of barley, Lupin or Chickpea grown in newly reclaimed soil. Egyptian journal of applied science 25(9), 437-452.

Ndakidemi PA. 2006. Manipulating legume/cereal mixtures to optimize the above and below ground interactions in the traditional African cropping systems, African Journal of Biotechnology 5 (25), 2526-2533.

Nyoki D, Ndakidemi PA. 2014a. Effects of Phosphorus and Bradyrhizobium japonicum on Growth and Chlorophyll Content of Cowpea (Vigna unguiculata (L) Walp), American Journal of Experimental Agriculture 4(10), 1120-1136.

Nyoki D, Ndakidemi PA. 2014b. Effects of Bradyrhizobium japonicum and Phosphorus Supplementation on the Productivity of Legumes, International Journal of Plant and Soil Science 3(7), 894-910.

Onduru DD, De Jager A, Muchena FN, Gachini GN, Gachimbi L. 2008. Exploring Potentials of Rhizobium Inoculation in Enhancing Soil Fertility and Agro-economic Performance of Cowpeas in Sub-saharan Africa: A Case Study in Semi-arid Mbeere, Eastern Kenya. American-Eurasian Journal of Sustainable Agriculture 2(3), 187-195.

Pirlak L, Kose M. 2009. Effects of plant growth-promoting rhizobacteria on yield and some fruit properties of strawberry. Journal of Plant Nutrition 32(7), 1173-1184. 60902943197.

Raj SN, Deepak SA, Basavaraju P, Shetty HS, Reddy MS, Kloepper JW. 2003. Comparative performance of formulations of plant growth–promoting rhizobacteria in growth promotion and suppression of downy mildew in pearl millet. Crop Protection 22, 579-588. S0261-2194(02)00222-3.

Shahid MQ, Saleem MF, Khan HZ, Anjum SA. 2009. Performance of soybean (Glycine max L.) under different phosphorus levels and inoculation. Pakistan Journal of Agricultural Sciences 46(4), 237-241.

Soleymani A, Mohammad HS. 2012. Forage yield and quality in intercropping of forage corn with different cultivars of be rseem clover in different levels of nitrogen fertilizer. Journal of Food, Agriculture and Environment 10(1), 602-604.

Steel RGD, Torrie JH. 1980. Principles and Procedures of Statistics: A Biometrical Approach,” 2nd Edition, McGraw-Hill Kogakusha, New York.

Undie UL, Uwah DF, Attoe EE. 2012. Effect of intercropping and crop arrangement on yield and productivity of late season Maize/soybean mixtures in the humid environment of South Southern Nigeria. Journal of Agricultural Science 4(4), 37-50.

Verma JP, Yadav J, Tiwari KN. 2012. Enhancement of Nodulation and Yield of Chickpea by Co-inoculation of Indigenous Mesorhizobium spp. and Plant Growth–Promoting Rhizobacteria in Eastern Uttar Pradesh, Communications in Soil Science and Plant Analysis 43(3), 605-621

Vincent JM, Nutman PS, Skinner FA. 1979. The identification and classification of Rhizobium. In: Shinner FA, Lovelock DW (Eds) Methods for microbiologists. (Society for applied bacteriology, technical series no 14) Academic Press, London.

Wagner SC. 2011. Biological Nitrogen Fixation. Nature Education Knowledge 3(10), 15. Available at

Wall LG, Helster A, Huss-Danell K. 2000. Nitrogen Phosphorus and the ratio between them affect Nodulation in Alnos increase and S. pratense. Symbiosis 29(2), 91-105.

Wu FY, Wan J, Wu S, Lin X, Wong M. 2013. Inoculation of Earthworms and Plant Growth–Promoting Rhizobacteria (PGPR) for the Improvement of Vegetable Growth via Enhanced N and P Availability in Soils, Communications in Soil Science and Plant Analysis 44(20), 2974-2986,

Yamanaka T, Akama A, Li CY, Okabe H. 2005. Growth, nitrogen fixation and mineral acquisition of Alnussieboldiana after inoculation of Frankia together with Gigaspora margarita and Pseudomonas putida. Journal of Forest Research 10(1), 21-26.

Yasmeen S, Bano A. 2014. Combined Effect of Phosphate-Solubilizing Microorganisms, Rhizobium and Enterobacter on Root Nodulation and Physiology of Soybean (Glycine max L.), Communications in Soil Science and Plant Analysis, 45(18), 2373-2384,