Hepatoprotective potential of dichloromethan extract of Cassia alata L. (Caesalpiniaceae) leaves from Burkina Faso against carbon tetrachloride-induced hepatic damage in rats

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Hepatoprotective potential of dichloromethan extract of Cassia alata L. (Caesalpiniaceae) leaves from Burkina Faso against carbon tetrachloride-induced hepatic damage in rats

Da Filkpièrè Léonard, Obama Pierre, Ouédraogo Youssoufou, Belemtougri Gourounga Raymond, Dimo Théophile
Int. J. Biosci.11( 5), 43-53, November 2017.
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Cassia alata L. is an herbal plant that has been used as hepatoprotective and anti-inflammatory agent in Burkina Faso medicine. The purpose of this study is to finalize a traditionally improved drug (MTA) that will serve in the treatment of the hepatic disorders. Rats were orally fed with CF-AECal (50 and 100 mg/kg bw) along with administration of CCl4 (20% CCl4/ olive oil; 0.5 ml/kg bw) for 6 weeks.The protection level of hepatic cells by CF-AECal is evaluated by blood rate of SGPT, SGOT, APL and TB and also tissues rate of MDA, GSH, SOD and CAT. This protection rate is observed on the tissues through histological sections.The results showed that the levels of SGPT (12.64 ± 0.25), SGOT (13.13 ± 0.88), ALP (20.39 ± 0.44), TB (12.21 ± 0.30), were significantly increased in CCl4 treated rats when compared with the normal group, but CF-AECal (50 and 100 mg/kg) treated rats showed maximum reduction of SGPT (6.71 ± 0.58 and 6.42 ± 0.49), SGOT (11.25 ± 0.45 and 10.79 ± 0.51), ALP (18.50 ± 0.76 and 16.08 ± 1.49), total bilirubin (10.95 ± 0.62 and 10.36 ± 0.13). Also CF-AECal significantly reduced liver MDA, GSH, SOD and CAT content when compared with control. Histopathological examination of liver sections confirmed that, treatment with CF-AECal decreased the degree of hepatic damage induced by CCl4. Conclusion: these results suggest that the extract of C. alata leaves at various doses protects the rats from CCl4 effects due to its polyphenol and particularly its flavonoids.


Adewusi EA, Afolayan AJ. 2010. A review of natural products with hepatoprotective activity. Journal of Medicinal Plants Research; 4(13), 1318-1334. http://www.academicjournals.org/JMPR

Arijit M, Sanjay KK, Tanushree S, Rajalingam D, Tapan KM. 2012. Evaluation of hepatoprotective effect of leaves of Cassia sophera Linn.Evidence-Based Complementary and Alternative Medicine; 2012, 5. http://dx.doi.org/10.1155/2012/436139

Awang D. 1993. Milk Thistle. Canadian Pharmacists Journal 23, 749-754.

Banchroft JD, Stevens A, Turner DR. 1996. Theory and practice of histological techniques. Fourth ed. Churchil Livingstone, New York, London, San Francisco, Tokyo, 125 p.

Baravalia, Yogesh K. 2010. Evaluation of anti-inflammatory and hepatoprotective potency of a selected medicinal plant. Saurashtra University Theses Service http://etheses.saurashtrauniversity.edu

Boyd SC, Sasame HA, Boyd MR. 1981. Gastric glutathione depletion and acute ulcerogenesis by diethylmaleate given subcutaneously to rats. Life Sciences; 28(26), 2987–2992. http://dx.doi.org/10.1016/0024-3205(81)90276-9

Choi EY, Hwang HJ, Kim IH, Nam TJ. 2009. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats. Food and Chemical Toxicology; 47(1), 134-139. http://dx.doi.org/10.1016/j.fct.2008.10.026

Crockett CO, Guede-Guina F, Pugh D, Vangah-Manda M, Robinson J, Qlubadewo JO. 1992. Cassia alata and the preclinical search for therapeutic agents for the treatment of opportunistic infections in AIDS patients. Cellular and Molecular. Biology 35(5), 505-511.PMID: 1468110

Ellman GL. 1959. Tissue sulfhydryl group.Archives of biochemistry and biophysics 82(1), 70-77. http://dx.doi.org/10.1016/0003-9861(59)90090-6

Esimone CO, Nworu CS, Ekong US, Okereke BC. 2008. Evaluation of the antiseptic properties of Cassia alata-based herbal soap. The Internet Journal of Alternative Medicine 6(1), 117-24. http://ispub.com/IJAM/6/1/5856

Guillouzo A, Clerc C, Malledant Y, Chesne C, Tatanasavanh D, Gugen- Guillouzo C. 1989. Modèles d’étudesde la cytoprotection technique. Gastroentérologie Clinique et Biologique Journals 13, 725-730.

Handa SS, Sharma A, Chakarborty KK. 1986. Natural Products and plants as liver protecting drugs. Fitoterapia 57(5), 307-352.

Hernandez-Munoz R, Diaz-Munoz M, Suarez-Cuenca JA, Trejo-Solis C, Lopez V, Sanchez-Sevilla L, Yanez L, De Sanches VC. 2001. Adenosine reverses a preestablished CCl4-induced micronodular cirrhosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats. Hepatology 34(4), 677– 687.

Kingsley CPI, Matthew OW, Talat M. 2011.Hepatoprotective effect of crude methanolic extract and fractions of Ring worm plant Senna alata (L. Roxb) leaves from Nigeria against carbon tetrachloride –induced hepatic damage in rats. European Journal of Experimental Biology, 1(1), 128-138.

Lin HM, Tseng HC, Wang CJ, Lin JJ, Lo CW, Chou FP. 2008. Hepatoprotective effects of Solanumnigrum Linn extract against CCl(4)-induced oxidative damage in rats. Chemico-Biological Interactions. 15, 171(3), 283-93. https://doi.org/10.1016/j.cbi.2007.08.008

Manjunatha BK. 2006. Hepatoprotective activity of PterocarpussantalinusL.f.,an endangered medicinal plant. Indian Journal of Pharmacology 38 (1), 25–28. http://www.ijponline.com/text.asp?2006/38/1/25/19848

Misra, Fridovich. 1972. Determination of the level of superoxide dismutase in whole blood. Yale University, Press New Haven: 101-109

Moore D, Jun Z, Werdong H. 2004.Induction of bilirubin clearance by xenobiotic receptor CAR. Journal of Clinical Investigation; 113, 137-143.

Mohammed FA, Srinivasa Rao A. 2013. Comparative Hepatoprotective Activities of Selected Indian Medicinal Plants. Global Journal of Medical Research Pharma, Drug Discovery, Toxicology and Medicine; 13(2), 1-22.

Morazzoni P, Bombardelli E. 1995. Silybium marianum (Carduus marianus). Fitoterapia 66(1), 3–42. INIST : 16120, 35400005624938.0010. Nº notice refdoc (ud4) : 3477621

Moselhy SS, Ali HK. 2009. Hepatoprotective effect of cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats. Biological Research; 42(1), 93-98. https://doi.org/s0716-97602009000100009

Muriel P, Mourelle M. 1990. Prevention by silymarin of membrane alterations in acute CCl4 liver damage.Journal Applied Toxicology. 10(4), 275-279.PMID: 1975258

Muriel P, Alba N, Perez-Alvarez VM, Shibayama M, Tsutsumi VK. 2001. Kupffer cells inhibition prevents hepatic lipid peroxidation and damage induced by carbon tetrachloride. Comparative Biochemistry and Physiology. Toxicology & pharmacology; 130(2), 219-26. PMID:11574291.

Nacoulma/Ouédraogo OG. 1996. Plantes médicinales et Pratiques médicales Traditionnelles au Burkina Faso: Cas du plateau central T1 & T2. Thèse Doct. d’Etat ès Sciences Nat. Université de Ouagadougou, 91p et 92p.

Navarro VJ, Senior JR. 2006.Drug-related hepatotoxicity. New England Journal of Medicine; 354, 731-9. http://www.nejm.org/doi/full/10.1056/NEJMra052270

Palanichamy S, Nagarajan S. 1990. Antifungal  activity of Cassia alata leaf extract. Journal of Ethnopharmacology; 29(3), 337-340. PMID:2214817

Pang S, Xin X, Stpierre MV. 1992. Determinants of metabolic disposition. Annual Review of Pharmacology and Toxicology 32, 625–26. https://doi.org/10.1146/annurev.pa.32.040192.003203

Parthasarathy G, Prasanth V. 2009. Hepatoprotective Activity of Cassia fistula Linn. Bark Extracts against Carbon Tetra Chloride Induced Liver Toxicity in Rats. Internet Journal of Pharmacology; 6, 2.

Pradeep Kumar Samal. 2013. Hepatoprotective activity of Ardisia solanacea in CCl 4 induced Hepatoxic albino rats. Asian Journal of Pharmaceutical Sciences 3(2), 79-82. http://www.asianpharmaonline.org

Premitha Abraham P, Wilfed G, Ramakrishna B. 1999. Decreased activity of hepatic alkaline proprotease in rats with carbon tetrachloride induced livercirrhosics. Indian Journal of Experimental Biology 37, 1234-1244.

Ramellini G, Meldolesi J. 1976. Liver protection by silymarin. In vitro on dissociated rat hepatocytes. Arzneimittel Forschung (Drug Research); 26(1), 69–73. PMID:947182

Recknagel RO, Glende JEA, Dolak JA, Waller RL. 1989. Mechanisms of carbon tetrachloride toxicity. Pharmacology & Therapeutics 43(1), 139–154.PMID: 2675128.

Ross MH, Romrell LJ, Kaye GI. 1995. Digestive system II: Esophagus and gastrointeinal tract. In histology, a text and atlas.3rd edition, chapter 16, p.440-495.

Scevola D, Baebacini GM, Grosso A, Bona S, Perissoud D. 1984. Flavonoids and hepatic cyclique monophosphates in liver injury. Bollettino Istituto Sieroterapico Milanese 63, 77–82.

Sheeba M, Emmanuel S, Revathi K, Ignacimuthu S. 2009. Wound healing activity of Cassia occidentalis L. in albino Wistar rats. International Journal of Integrated Biology 8(2), 1‐6.

Sinha KA. 1972. Colorimetric essay of catalase. Analytical Biochemistry; 47(2), 389-394. http://dx.doi.org/10.1016/0003-2697(72)90132-7

Somchit MN, Reezal I, Nur IE, Mutalib AR. 2003. In vitro antimicrobial activity of ethanol and water extracts of Cassia alata. Journal of Ethnopharmacology; 84(1), 1–4.PMID: 12499068

SureshKumar SV, Mishra SH. 2008. Hepatoprotective effect of Pergularia daemia (Forsk.) ethanol extract and its fraction.Indian Journal of Experimental Biology; 46(6), 447- 452. https://www.ncbi.nlm.nih.gov/pubmed/18687603

Swierkosz TA, Mitchell JA, Warner TD, Botting RM, Vane JR. 1995. Co-induction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids. British Journal Pharmacology 114(7), 1335-1342. https://www.ncbi.nlm.nih.gov/pmc/articles/pmc1510271/

Thabrew MI, Joice PDTM, Rajatissa WAA. 1987. Comparative Study of the Efficacy of Pavettaindica and Osbeckiaoctandra in the Treatment of Liver Dysfunction.PlantaMedica 53, (3), 239-241. http://dx.doi.org/10.1055/s-2006-962691

Tran QI, Adnyana IK, Tezuka Y, Nagaoka T, Tran QK, Kadota SJ. 2001. Triterpenesaponins from Vietnamese gingeng (Panaxvietnamensis) and their hepatocytoprotective activity. Journal of Natural Products; 64(4), 456–461. http://pubs.acs.org/doi/abs/10.1021/np000393f

Vane JR, Mitchell JA, Appleton I, Tomlinson A, Bishop-Bailey D, Croxtall J, Willoughby DA. 1994. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proceedings of the National Academy of Sciences of the United States of America 91(6), 2046-2050. http://dx.doi.org/10.1073/pnas.91.6.204

Vijayan P, Prashanth HC, Vijayaraj P, Dhanaraj SA, Badami S, Suresh S. 2003. Hepatoprotective Effect of the Total Alkaloid Fraction of Solanumpseudocapsicum Leaves. Pharmaceutical Biology; 41(6), 443–448. http://dx.doi.org/10.1076/phbi.41.6.443.17827.

Ward FM, Daly MJ. 2005. Liver disease. In: Roger walker, Clive Edwards. Churchil Livingstone, New York. Clinical Pharmacy and Therapeutics; 3, 209-228

Wegener T, Fintelmann V. 1999.Flavonoids and Bioactivity.Wiener Medizinische Wochenschrift; 149, 241-247.

Wilbur KM, Bernheim F, Shapiro OW. 1949. Determination of lipid peroxidation. Archives of Biochemistryand Biophysics 24, 305-310.

Wolf PL. 1999. Biochemical diagnosis of liver disease. Indian Journal of Clinical Biochemistry 14(1), 59–90. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3453559/