Identification and characterization of abiotic stress responsive genes in Ricinus communis L. using bioinformatics tools

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Research Paper 01/05/2020
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Identification and characterization of abiotic stress responsive genes in Ricinus communis L. using bioinformatics tools

Wajia Noor, Bibi Sadia, Iftikhar Ahmad Baloch, Asyia, Junaid Ahmad, Bibi Safia, Farida
Int. J. Biosci.16( 5), 23-34, May 2020.
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Bioinformatics is one of the leading fields in biology for genomics research. In the present research bioinformatics analysis of abiotic stress responsive genes investigation was carried out in Ricinus communis L. Selected twenty-seven genes of model plant Arabidopsis thaliana were searched in Ricinus communis L. The investigated genes belonged from salt, heat, cold and drought stress. Genes were find out using insilico analysis. Homology search was applied using a best available tool of NCBI called BLAST. Homology search gave best results up to 97% query coverage (ANAH-like) for the presence of selected model plant genes in Ricinus communis L. The query coverage results varied from 2% to 97%. Seven genes ANAH (97%), CSD1 (93%), TAF1 (82%), SOS4 (70%), HOS10 (61%) and MPK3 (61%) expressed high query coverage in R. communis L. Ten genes HVA22 (59%), SOS2 (49%), MYBR1 (45%), HOS31 (49%), STZ (42%), DDF1 (41%), BO1 (41%), LOS4 (39%), CDPK1 (37%) and KIN11 (35%) were somewhat similar. While remaining genes expressed similarity less than 30%. Besides query coverage the investigation of conserved domains showed that these predicted genes exhibited conserved domains similar to that of model plant genes. Phylogenetic analysis of some selected genes confirm the genes existence and gave evolutionary aspect of the gene. The identification of abiotic stress responsive genes makes good contribution to the research on this plant and will help to understand the mechanism to respond the stress in this plant.


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