Identification of chemical compounds cherry leaves (Muntingia calabura) powder as a natural antioxidant

Paper Details

Research Paper 01/05/2017
Views (1576) Download (126)
current_issue_feature_image
publication_file

Identification of chemical compounds cherry leaves (Muntingia calabura) powder as a natural antioxidant

Diana Triswaningsih, Sri Kumalaningsih, Wignyanto, Pratikto
Int. J. Agron. Agri. Res.10( 5), 84-91, May 2017.
Certificate: IJAAR 2017 [Generate Certificate]

Abstract

Antioxidant compound has a small molecular weight but is able to inhibit the oxidation reaction by preventing the formation of radicals. Using of synthetic antioxidants continuously can cause negative effect for the body. Based on the reason we needed another source of natural antioxidant by using plants, and one of plant that can we used is cherry leaf. Cherry leaf is an annual plant is easy to grow and contains a lot of phenolic compounds. This study showed that the cherry leaf extract contains flavanoid compounds, terpenoids, tannins and high antioxidant activity. Cherry leaf extract could be substitute synthetic antioxidants with further processing and identified constituent compounds. This study aims to determine the constituent compounds cherry leaf powder using format method of drying through cherry leaf extract at 50 °C and then homogenized with maltodextrin 8%, 0.3% tween 80 and dried at a drying temperature of 50 °C. The results obtained demonstrate the value of using the DPPH antioxidant activity of 80.50%. Identification of powdered Cherry leaf constituent compounds by GC-MS analysis shows the components of the volatile compounds such as geraniol (26,335%), eugenol (19,950%), citronellol (16,958%), α-amyrin (6,225%), myrcene (3,440%) and α-terpineol (7,356%).  While the identification of constituent compounds by LC-MS analysis shows the phenol compounds: Gallic acid (18,607%), Catechin (14,077%), Quercetin (10,255%), Ellagic acid (9,626%) and Kaempferol (8,699%). Result of analysis functional compound with infrared wave length range of 520.74 to 3417.63 cm-3, 15. The infra-red spectrum of functional compounds and the presence of broad bands at 3417.63 cm-3 can be attributed to (OH) stretching.

VIEWS 478

Agati G, Azzarello E, Pollastri S, Tattini M. 2012. Flavo- noids as Antioxidants in Plants: Location and Functional Significance.  Plant Science 196, 67-76. DOI: 10.1016/j.plantsci.2012.07.014

Anilakumar KR, Sudarshanakrishna KR, ChandramohanG, Ilaiyaraja N, Khanun F, Bawa AS. 2010. Effect of Aloe vera (L.) gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rat. Indian Journal of Experimental Biology 48, 837-842.

Arum YP.  2012. Isolasi dan Uji Daya Antimikrobia Ekstrak Daun Kersen  (Muntingia calabura). Under Graduates thesis, Universitas Negeri Semarang. Jurnal MIPA 35(2), 165-174 (In Bahasa Indonesia)

Atmane M, Muriel J, Jo lS, Stephane D. 2006. Flavour Encapsulation and Controlled Release – A Review. Review International Journal of Food Science and Technology 41, 1–21.

Cai YZ, Sun M, Corke H. 2003.  Antioxidant Activity of Betalains from Plants of the Amaranthaceae, Journal of Agricultural and Food Chemistry 51(8), 2288-2294. DOI: 10.1021/jf030045u

Cai Y, Luo Q, Sun M, Corke H. 2004 Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science 74, 2157-2184. DOI: 10.1016/ j.lfs. 2003. 09.047

Carocho M, Ferreira ICFR. 2013. A Review on Anti- oxidants, Prooxidants and Related Controversy: Natural and synthetic compounds. Screening and Analysis Methodologies and Future Perspectives.  Food and Chemical Toxicology 51, 15-25. DOI: 10. 1016/j.fct.2012.09.021

Chiang CJ,  Kadouh H, Zhou KQ. 2013. Phenolic Com- pounds and Antioxidant Properties of Gooseberry as Af- fected by in Vitro Digestion,” LWT-Food Science and Te- chnology 51(2), 417-422. DOI: 10.1016/j.lwt.2012.11.014

EFSA. 2012. Scientific Opinion on the Reevaluation of Buty- lated Hydroxytoluene BHT (E 321) as a Food Additive. EFSA Panel on Food Additives and Nutrient Sources Ad- ded to Food (ANS).  European Food Safety Authority Journal 10(3), 2588. www.efsa.europa.eu/en/efsajournal/doc/2588.pdf

Elzanfaly ES, Hanan AM. 2017. A Liquid Chromatography/Tandem Mass Spectrometric Method for Determination of Captopril in Human Plasma: Application to a Bioequivalence Study. Journal of Applied Pharmaceutical Science 7(02), 008-015.

Espín J, Tomás-Barberán F. 2005. Constituyentes bioactivos no-nutricionales de alimentos de origen vegetal y su aplicación en alimentos funcionales, in n.d. (Ed.), Alimentos funcionales. Fundación Española para la Ciencia y la Tecnología [FECYT], Madrid, 101-153.

Foyer  CH, Noctor G. 2003. Redox Sensing and Signal- ling Associated with Reactive Oxygen in Chloroplasts, Pe-roxisomes and Mitochondria. Physiologia Plantarum 119(3), 355-364. DOI: 10. 1034/j.1399-3054.2003.00223.x

Jafari SM, Assadpoor E, He Y, Bhandari B. 2008. Encapsulation Efficiency of Food Flavours and Oils during Spray Drying. Journal Drying Technology 26, 816-835.

Janusz A, Ewelina M. 2004. Mikroencapsulation of Oil Matrix/Water System During Spray Drying Process-Proceedings of the 14th International Drying Symposium (IDS 2004). C: 2043-2050

Kasma I. 2007. Kajian Pengolahan Bubuk Instant Bubuk Wortel Dengan Metoda Foammat Drying. Balai Pengkajian Teknologi Pertanian Sumatera Barat. Buletin Teknologi Pascapanen Pertanian 3, 20-25.

Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. 2008. Antioxidant Activity of Some Common Plants. Turky Journal Biology 51, 55.

Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H. 2002. Antioxidants Properties of Ferulic Acid and Its Related Compound. J. Agric. Food Chem 50, 2161-2168.

Krishnaveni, Marimuthu, Ravi Dhanalakshmi.  2014. Qualitative and Quantitative Study of Phytochemicals in Muntingia calabura L. Leaf and Fruit. World Journal of Pharmaceutical Research 3(6),   1687-1696.

Kuntorini, Evi Mintowati, Setya Fitriana dan Maria Dewi Astuti. 2013. Struktur Anatomi Dan Uji Aktivitas  Antioksidan Ekstrak Metanol Daun Kersen (Muntingia Calabura). Prosiding Semirata FMIPA Universitas Lampung, 2013. Semirata 2013 FMIPA Unila (In Bahasa Indonesia)

Lutfiadi R, dan Supriyadi. 2007. Model Matematik Perubahan Senyawa Geraniol Teh Hijau Selama Penyimpanan. www.ejournal-unisma.net 8(1) (In Bahasa Indonesia)

Naiyana P, Sunisa S, Worapong U, Saowakon W. 2010. Effect of Thermal Processing and Protein Nutriens on Antioxidant Activity of Tom-Kha Paste Extract. Asian Journal Food AfrgroIndustry 3 (04), 389-399.

Narchi A, Ch,  Vial AG, Djelveh. 2007. Effect of the Formulation on The Continuous Manufacturing of Foamed Products. Proceedings of European Congress of Chemical Engineering (ECCE-6) Copenhagen. 1-17.

Paiva MF, Gordon MH. 2002. Effect of pH and Ferric Ions on The Antioxidant Activity of Olive Polyphenols in Oil-in-Water Emulsions. JAOCS 79(6), 571-576.

Patras A, Burton NP, Pieve SD, Butler F. 2009. Impact of high pressure processing on total antioxidant activity, phenolic, ascorbic acid, anthocyanin content and colour of strawberry and blacberry purees. Innovative Food Science and Emerging Technologies 10, 308-313

Rajkumar P, Kailappan R, Viswanathan R, Rghavan GSV. 2007. Drying characteristics of foamed alphonso mango pulp in continous type foammat dryer. Journal of Foo Engineering 79 (4), 1452-1459.

Ratti C, Kudra T. 2008. Process and Energy Optimazition In Drying Foamed Material. Canmet Energy Technology Centre- Varennes, PQ, Canada J3X 1S6 Department of Soils and Agri-Food Engineering, Laval University, Quebec, QC, Canada G1K 7P4. Journal Drying Technology 66, 47.

Sani MH, Mohd ZA, Zakaria T, Balan LK, The, Salleh MZ. 2012. Antinociceptive Activity of Methanol Extract of Muntingia calabura Leaves and the Mechanisms of Action Involved. Research Article Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Article ID 890361, 10.

Shebis Yevgenia, David Iluz, Yael Kinel-Tahan, Zvy Dubinsky, Yaron Yehoshua. 2013. Natural Antioxidants: Function and Sources. Food and Nutrition Sciences 4, 643-649.

Shih  Cheng-Dean,  Jih-Jung Chen, Hsinn-Hsing Lee†.  2006. Activation of Nitric Oxide Signaling Pathway Mediates Hypotensive Effect of Muntingia calabura L. (Tiliaceae) Leaf Extract. The American Journal of Chinese Medicine 34(5), 857–872.

Siddiqua  Ayesha KB, Premakumari,  Rokeya sultana, Vithya and Savitha. 2010. Antioxidant Activity and  Estimation of Total Phenolic  Content of  Muntingia calabura by Colorimetry. International Journal of Chem Tech Research CODEN( USA): IJCRGG ISSN : 0974-4290 2(1), 205-208.

Sridhar M, Thirupathi K, Chaitanya G, Ravi Kumar B, Krishna Mohan G. 2011. Antidiabetic Effect of Leaves of Muntingia calabura L, Normal and Alloxan Induced Diabetic Rats. Pharmacologyonline 2, 626-632.

Wartini NM. 2007. Komparasi Metoda Separasi dan Pengaruh Curing Terhadap Senyawa Penentu Flavor Pada Ekstrak Falor Daun Salam. Disertasi.Program Pasca Sarjana Universitas Brawijaya.Malang (In Bahasa Indonesia).

Winarsi H. 2007. Antioksidan Alami dan Radikal Bebas. Yogyakarta: Kanisius. (In Bahasa Indonesia).

Wong CC, Li HB, Cheng KW, Chen F. 2006. A Sys- tematic Survey of Antioxidant Activity of 30 Chinese Medicinal Plants Using the Ferric Reducing Antioxidant Power Assay. Food Chemistry 97(4), 705-711. DOI: 10.1016/j.foodchem.2005.05.049

Yuslinda, Elka. 2012. Penetuan Aktivitas Antioksidan Dari Beberapa Ekstrak Sayur-sayuran Segar dan Dikukus Dengan Metode DPPH. Scientia 2(1), 1. (In Bahasa Indonesia).

Zakaria ZA. 2007. Free Radical Scavenging Activity of Some Plants Available in Malaysia. Iranian Journal of  Pharmacology & Therapeutics 6(1), 87-91.

Zakaria ZA, Mohd Hijaz Mohd Sani, Manraj Singh Cheema, Arifah Abdul Kader, Teh Lay Kek, Mohd Zaki Salleh. 2014. Antinociceptive activity of methanolic extract of Muntingia calabura leaves: further elucidation of the possible mechanisms. Research article BMC Complementary and Alternative Medicine 14, 63.