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Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita

By: Shameem Shabana, Mayavan Karthika, Ponnusamy Suganya, Venkatachalam Ramasubramanian

Key Words: Klebsiella pneumonia, Gentamicin, Ofloxacin, Kanamycin, Tetracycline

Int. J. Biosci. 12(1), 42-50, January 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.1.42-50

Certification: ijb 2018 0185 [Generate Certificate]

Abstract

The microbiological studies on freshwater fish culture appear to be limited predominantly in India. Consequently a study was done to identify the bacterial populations and pathogens in the sample water and in some tissues of cultured carp Labeo rohita. Among the various organs analyzed, the maximum bacterial load was found in gills followed by intestine and skin surface of the infected Labeo rohita. In general, the bacteria isolated from fish samples appeared to be very similar to those obtained from water. The Bacterial isolates were confirmed based on the morphological, Physiological and Biochemical Characteristics using standard methods. In biochemical test it is observed, that the bacteria was 90 percentage confirmed as Klebsiella genus which is gram negative and rod shape. For further confirmation, the bacterial sample have been inoculated with specific selective medium (Mac Conkey Agar Medium) and confirmed that the bacteria as Klebsiella pneumoniae by the appearance of the colony and change in the colour of the medium. Among the 13 antibiotic discs, certain antibiotics were sensitive such as Gentamicin-32mm, Ofloxacin-29mm, kanamicin-28mm and tetracycline-25mm against the pathogenic bacteria K. pneumoniae. This present study shows that Gentamicin, Ofloxacin, Kanamycin and Tetracycline can be used as a drug against the pathogenic bacteria K. pneumoniae for the infected fish (Labeo rohita).The above mentioned antibiotic disc against K. pneumoniae induced the growth of Labeo rohita and promote the aquaculture production and increase the economic status of aquaculture.

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Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita

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Shameem Shabana, Mayavan Karthika, Ponnusamy Suganya, Venkatachalam Ramasubramanian.
Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita.
Int. J. Biosci. 12(1), 42-50, January 2018.
https://innspub.net/ijb/antibiogram-pathogenic-bacteria-isolated-identified-edible-fish-labeo-rohita/
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