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Effects of chlorpyrifos on testicular biochemistry and physiology of male sprague dawely rats

By: Zeeshan Laghari, Nimra Munir, Maryam Hayat, Bahzad Ahmad Farhan, Huda Zahoor, Sidra Hameed, Irha Basit, Muhammad Irfan, Ansa Yaqoob, Muhammad Humayun Ajaz, Usman Ghani

Key Words: Chlorpyrifos, Testicular Biochemistry, Organophosphate Pesticides, Histopathology, Hematology, Spermatogonia.

Int. J. Biosci. 17(1), 75-87, July 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.1.75-87

Certification: ijb 2020 0008 [Generate Certificate]

Abstract

Pesticides and insecticides are essential piece of our organobusiness framework against the pests and insects to get better yields of crops. On the other hand, chemicals and reagents used in pestisides and insectisides are harmful and carcinogenic for other living organisms chlorpyrifos is broadly used organophosphate pesticide throughout the world but too much use of this organophosphate may have serious health issues and it is examined that it may cause infertility in the human beings. So it is important to control over and misuse of these pesticides. Therefore, this research was proposed to examine the hazardous effects of CPF on health of living organisms. Specially, male albino rats were used to check the degenerative effects of CPF on the testis. Different groups of male rates were exposed with CPF drug with different concentrations orally for 28 days of trial on consistent schedule. After completion of successful trial blood samples and testis sample were taken by disecting each rat according to their groups and hematological and biochemical analysis uncovered noteworthy damaging effects of CPF in the blood samples. Hematological and serum compound profiles of rats show increment in WBC’s, PLT, LYM, Total Bilirubin, Glucose, ALP, ALT, and AST. Generous diminishing in the dimensions of HCT, MCV, MCH, MCHC, RBC’s and HGB was noticed. Histology of testis of rats under microscopic observation shows the degenerative effects of CPF on the spermatogonial cell of testis as compare to the control group. So current investigation suggests that chlorpyrifos may cause histological, hematological and biochemical changes in the living organisms and should be banned over or misuse of CPF by world health organisations.

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Effects of chlorpyrifos on testicular biochemistry and physiology of male sprague dawely rats

Bernardi C, Monetal, D Brughera M, Di Salvo M, Lamparelli D, Mazue G, Iatropoulos MJ. 1996. Haematology and clinical chemistry in rats: comparison of different blood collection sites. Comparative Haematology International 6a (3), 160-166.

Carleton HM, Drury RAB, Wallington EA. 1967. Carleton’s Histological Technique: Rev. and Rewritten by RAB Drury [and] EA Wallington. Oxford University Press.

Chauhan RS, Singhal L. 2006. Harmful effects of pesticides and their control through cowpathy. International Journal of Cow Science 2(1), 61-70.

Drury RAB, Wallington EA, Cameron SR. 1967. Carleton’s histological technique. London.

Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Neubert D. 2008. Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Critical reviews in toxicology 38(sup2), 1-125.

Eddleston M, Buckley NA, Eyer P, Dawson AH. 2008. Management of acute organophosphorus pesticide poisoning. The Lancet 371(9612), 597-607.

Eddleston M, Gunnell D, Karunaratne A, De Silva D, Sheriff MR, Buckley NA. 2005. Epidemiology of intentional self-poisoning in rural Sri Lanka. The British Journal of Psychiatry 187(6), 583-584.

Eddleston M. 2000. Patterns and problems of deliberate self‐poisoning in the developing world. Qjm 93(11), 715-731.

Ergonen AT, Salacin S, Ozdemir MH. 2005. Pesticide use among greenhouse workers in Turkey. Journal of Clinical Forensic Medicine 12(4) 205-208.

Friedmann GB, Algard FT, McCurdy HM. 1969. Determination of the red blood cell count and haemoglobin content of urodele blood. The Anatomical Record, 163(1) 55-57.

Gibson KH. 1998. U.S. Patent No. 5,770,599. Washington, DC: U.S. Patent and Trademark Office.

Gofman JW, Delalla O, Glazier F, Freeman NK, Lindgren FT, Nichols AV, Tamplin AR. 2007. The serum lipoprotein transport system in health, metabolic disorders, atherosclerosis and coronary heart disease. Journal of clinical Lipidology 1(2), 104-141.

Hernández AF, Parrón T, Tsatsakis AM, Requena M, Alarcón R, López-Guarnido O. 2013. Toxic effects of pesticide mixtures at a molecular level: their relevance to human health. Toxicology 307, 136-145.

Joshi SC, Mathur R, Gulati N. 2007. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicology and Industrial Health 23(7), 439-444.

Lajmanovich RC, Attademo AM, Simoniello MF, Poletta GL, Junges CM, Peltzer PM, Cabagna-Zenklusen MC. 2015. Harmful effects of the dermal intake of commercial formulations containing chlorpyrifos, 2, 4-D, and glyphosate on the common toad Rhinellaarenarum (Anura: Bufonidae). Water, Air, & Soil Pollution 226(12), 427.

Landrigan PJ, Claudio L, Markowitz SB, Berkowitz GS, Brenner BL, Romero H, Wolff MS. 1999. Pesticides and inner-city children: exposures, risks, and prevention. Environmental health perspectives 107(suppl 3), 431-437.

Mahmood I, Imadi SR, Shazadi K, Gul A, Hakeem KR. 2016. Effects of pesticides on environment. In Plant, soil and microbes, p 253-269. Springer, Cham.

Mandour RA. 2012. Existence of insecticides in tap drinking surface and ground water in Dakahlyia Governorate, Egypt in 2011. Int J Occup Environ Med (The IJOEM), 3(1 January).

Mathur A, Tripathi AS, Kuse M. 2013. Scalable system for classification of white blood cells from Leishman stained blood stain images. Journal of pathology informatics, 4(Suppl).

McGovern JJ, Jones AR, Steinberg AG. 1955. The hematocrit of capillary blood. New England journal of medicine 253(8), 308-312.

Muller F, Drits V, Plançon A, Robert JL. 2000. Structural transformation of 2: 1 dioctahedral layer silicates during dehydroxylation-rehydroxylation reactions. Clays and Clay Minerals 48(5), 572-585.

Pimental MB. 1971. Heroin Maintenance: A Medical Overview. Bureau of Narcotics and Dangerous Drugs.

Rathod AL, Garg RK. 2017. Chlorpyrifos poisoning and its implications in human fatal cases: A forensic perspective with reference to Indian scenario. Journal of forensic and legal medicine 47, 29-34.

Sahli T. 1962. Text book of clinical pathology. Williams and Willams and Co., Baltimore, 35.

Sayım F. 2007. Histopathological effects of dimethoate on testes of rats. Bulletin of environmental contamination and toxicology 78(6), 479-484.

Schalm OW, Jain NC, Carroll EJ. 1975. Veterinary hematology (No. 3rd edition). Lea &Febiger.

Tanvir EM, Afroz R, Chowdhury MAZ, Gan SH, Karim N, Islam MN, Khalil MI. 2016. A model of chlorpyrifos distribution and its biochemical effects on the liver and kidneys of rats. Human & experimental toxicology 35(9), 991-1004.

Waziri MA, Ribadu AY, Sivachelvan N. 2010. Changes in the serum proteins, hematological and some serum biochemical profiles in the gestation period in the Sahel goats. Vet arhiv 80, 215-224.

Yadav IC, Devi NL, Syed JH, Cheng Z, Li J, Zhang G, Jones KC. 2015. Current status of persistent organic pesticides residues in air, water, and soil, and their possible effect on neighboring countries: A comprehensive review of India. Science of the Total Environment 511, 123-137.

Zhao Q, Dourson M, Gadagbui B. 2006. A review of the reference dose for chlorpyrifos. Regulatory Toxicology and Pharmacology 44(2), 111-124.

Zhou C, Li X. 2018. Cytotoxicity of chlorpyrifos to human liver hepatocellular carcinoma cells: effects on mitochondrial membrane potential and intracellular free Ca2+. Toxin Reviews 37(4), 259-268.

Zeeshan Laghari, Nimra Munir, Maryam Hayat, Bahzad Ahmad Farhan, Huda Zahoor, Sidra Hameed, Irha Basit, Muhammad Irfan, Ansa Yaqoob, Muhammad Humayun Ajaz, Usman Ghani.
Effects of chlorpyrifos on testicular biochemistry and physiology of male sprague dawely rats.
Int. J. Biosci. 17(1), 75-87, July 2020.
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