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In vitro antidiabetic, antiobesity and antioxidant activities of selected endemic plants from Mount Mayon and Mount Malinao Albay, Philippines

By: Lilibeth A Cajuday, Daile Meek S Membreve, Michael V Montealegre, Jocelyn E Serrano, Diomerl Edward Baldo

Key Words: Phytochemical components, Antioxidant activity, Alpha-glucosidase, Lipase inhibition

Int. J. Biosci. 16(3), 648-661, March 2020.


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The present study investigated the in vitro biological activities of the ethanol extracts of thirteen endemic medicinal plants in Albay against diabetes, obesity and as free radical scavenger. Alpha-glucosidase (AG), porcine pancreatic lipase (PPL) inhibition tests and DPPH radical scavenging assay were used to determine the antidiabetic, antiobesity and antioxidant properties of the extracts. Quantitative phytochemical analysis of the extracts was performed using standard methods. Of the 13 endemics, 6 plants exhibited potent inhibitory activity (>70%) against AG enzyme. Hydnocarpus alcalae, Merremia peltata, Trema orientalis, Cascabela thevetia, Stachytarpheta jamaicencis, and Ficus septica. S. jamaicensis has the lowest computed IC50 (0.8ug/ml) indicating highest AG inhibitory activity compared to the other plants and the drug Acarbose (1.88ug/ml). A total of 7 plants were found to have strong inhibitory activity of >70% against PPL: Leea guineensis, Solanum torvum, Cheilocostus speciosus, Melastoma malabathricum, Dendrocnide meyeniana, Cinnamomum mercadoi, and Gmelina arborea. L. guineensis which recorded the highest activity against PPL, contains very high amount of alkaloids (237.49mg/g AE), flavonoids (490.54mg/g QE), and phenols (220.59mg/g GAE) and also exhibited strong antioxidant activity in the DPPH assay. M. malabathricum has the lowest IC50 suggesting that it has the highest activity against PPL compared to the other plants and the drug Orlistat (1.81ug/ml). DPPH radical scavenging assay showed that all plant extracts possessed statistically (p≤0.05) higher antioxidant activity against ascorbic acid suggesting promising potent source of antioxidants. There is a need to conduct further study on the toxicity of the evaluated plants.

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In vitro antidiabetic, antiobesity and antioxidant activities of selected endemic plants from Mount Mayon and Mount Malinao Albay, Philippines

Aboaba SA, Choudhary IM. 2015. Chemical composition and biological activities of the volatile oils of Palisota hirsuta (Thunb) K. Schum and Trema orientalis (L) Blume. International Journal of Chemistry Vol. 7, No.

Ahmed D, Fatima M, Saeed S. 2014. Phenolic and flavonoid contents and anti-oxidative potential of epicarp and mesocarp of Lagenaria siceraria fruit: a comparative study. Asian Pacific Journal of Tropical Medicine 7 (Suppl 1), S249-S255.

Ajaiyeoba EO. 1999. Comparative phytochemical and antimicrobial studies of Solanum macrocarpum and Solanum torvum leaves, Fitoterapia 70, 184-186.

Al –Kattan MO, Khayyat SA. 2017. Antimicrobial activity and Chemical analyses of oil constituents of Medicinal Plant Costus speciosus (Koen.) Biomedical Research 28(2), 734-739.

Alvarez E, Leiro JM, Rodríguez M, Orallo F. 2004. Inhibitory effects of leaf extracts of Stachytarpheta jamaicensis (Verbenaceae) on the respiratory burst of rat macrophages. Phytotherapy Research 18(6), 457-462.

Ambujakshi HR, Heena T. 2009. Anthelminthic activity of Gmelina arborea leaves extract. International Journal of Pharmaceutical Research and Development 1(9), 1-3.

Andrade-Cetto A, Becerra-Jimenez J, Cardenas-Vazquez R. 2008. Alfa- glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes. Journal of Ethnopharmacology 116, 27-32.

Arthan D, Svasti J, Kittakoop P, Pittayakhachonwut D, Tanticharoen M, Thebtaranonth Y. 2002. Antiviral isoflavonoid sulfate and steroidal glycosides from the fruits of Solanum torvum. Phytochemistry 59, 459-463.

Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch, C, Uhrin P, Stuppner H. 2015. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances 33(8), 1582-1614.

Babatunde S, Michael WO, Oyindamola A. 2015. Bio-guided investigation of the antimalarial activities of Trema orientalis (L.) Blume leaves. African Journal of Biotechnology 14(43), 2966-2971.

Bhuyan B, Zaman K. 2008. Evaluation of hepatoprotective activity of rhizomes of Costus speciosus (J. Konig.) Smith. Pharmacology online 4(3), 119-126.

Brewer M. 2011. Natural antioxidants: sources, compounds, mechanism of action and potential applications. Comprehensive reviews in food science and food safety 10, 221-247.

Bueno PR, Buno CB, Santos DL, Santiago LA. 2013. Antioxidant activity of Ficus pseudopalma Blanco and its cytotoxic effect on hepatocellular carcinoma and peripheral blood mononuclear cells. Current Research in Biological and Pharmaceutical Sciences 2(2), 14-21.

Carriere F, Gargouri Y, Moreau H, Ransac S, Rogalska E, Verger R in: Wooley P, Petersen SB. (Eds.). 1994. Lipases, their structure, biochemistry and application, Book Cambridge University Press pp. 181-205.

Chan MFE, Geronimo AJO, Aspiras APF, Busaing EJW, Dato RJB, Calumpit DMA, Lafuente AT. 2016. Analysis of phytochemical, antimicrobial, and antioxidant properties of Sarcandra glabra (Thunb.) Nakai in relation to its ethnomedicinal relevance in Cordillera, Philippines. Indian Journal of Traditional Knowledge 15(3), 411-416.

Chang YT, Shen JJ, Wing WR, Yen HR. 2009. Alternative therapy for autosensitization dermatitis. Chang Gung Medicinal Journal 32(6), 668-673.

Chanmee W, Chaicharoenpong C, Petsom A. 2013. Lipase Inhibitor from fruits of Solanum stramonifolium Jacq. Food and Nutrition Sciences 4, 554-558.

Convention on Biological Diversity. 2009. Assessing progress towards the 2010 biodiversity target: The 4th National Report to the Convention on Biological Diversity The 4th National Report to the Convention on Biological Diversity.

Dechakhamphu A, Wongchum N. 2015. Screening for anti-pancreatic lipase properties of 28 traditional Thai medicinal herbs. Asian Pacific Journal of Tropical Biomedicine 5, 1042-1045.

Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. 2011. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis 24, 1043-1048.

Florido LV. 1978. Vegetative propagation by cuttings of yemane (Gmelina arborea Roxb.) using growth hormones. Sylvatrop Journal 3(2), 115-122.

Food and Nutriotion Research Institute (FNRI). 2011. 2nd National nutrition summit: 8th National nutrition survey. Food and Nutrition Research Institute Department of Science and Technology.

Fuentes RG, Diloy FN, Tan IL, Balanquit BJ. 2010. Antioxidant and Antibacterial Properties of Crude Methanolic Extracts of Cinnamomum mercadoi Vidal. Phil Journal of Natural Sciences 15, 9-15.

Galvez MA. 2015. Evaluation of DPPH free radical scavenging activity and phytochemical screening of selected folkloric medicinal plants in Tinoc, Ifugao, Cordillera Administrative Region, Philippines. International Journal of Scientific Research Publication 5(12), 440-445.

Gandhi GR, Ignacimuthu S, Paulrajmg, Sasikumar P. 2011. Antihyperglycemic activity and antidiabetic effect of methyl caffeate isolated from Solanum torvum Swartz. fruit in streptozotocin induced diabetic rats, European Journal of Pharmacology 30(23), 623-31.

Gerrath JM, Lacroix CR. 1997. Heteroblastic Sequence and Leaf Development in Leea guineensis. International Journal of Plant Sciences 158(6), 747-756.

DOI: 10.1086/297486.

Gondoin A, Grussu D, Stewart D, McDougall GJ. 2010. White and green tea polyphenols inhibit pancreatic lipase in vitro. Food Research International 43, 1537-1544.

Gorgonio SR, Fuentes RG. 2011. Antidiarrheal Activity of Cinnamomum mercadoi methanolic leaf and bark extracts. Philippine Journal of Natural Sciences 16-1, 43-47.

Gutiérrez SP, Sánchez MAZ, Gonzlález CP, García LA. 2007. Antidiarrhoeal activity of different plants used in traditional medicine. African Journal of Biotechnology 6(25), 2988-2994.

Hamuel J. 2012. Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic Agents, in Rao, V. (Ed.) Phytochemicals – A Global Perspective of Their Role in Nutrition and Health. InTech Open p. 538.

DOI: 10.5772/26052

Hayes JD, Eggleston IM. 2008. The cancer chemopreventive actions of phytochemicals derived from glucosinolates. European Journal of Nutrition 47 Suppl 2, 73-88.

Hu L, Yu W, Li Y, Prasad N, Tang Z. 2014. Antioxidant activity of extract and its major constituents from okra seed on rat hepatocytes injured by carbon tetrachloride. Biomed Research International 2014, 1-9.

Idu M, Erhabor JO, Odia EA. 2009. Morphological and anatomical studies of the leaf and stem of some medicinal plants: Stachytarpheta jamaicensis (L.) Vahl. And S. cayennensis (LC Rich) schau. Ethnobotanical Leaflets 13(11), 417-1425.

Ikeda K, Takahashi M, Nishida M. 2000. Homonojirimycin analogues and their glucosides from Lobelia sessilifolia and Adenophora spp. (Campanulaceae), Carbohydrate Research 323, 73-80.

Ikewuchi JC, Okaraonye CC, Ogbonnaya EA. 2009. Time course of the effect Stachytarpheta jamaicensis L. (Vahl.) on plasma sodium and potassium levels of normal rabbits. Journal of Applied Sciences Research 5(10), 1741-1743.

Institute for Clinical Systems (ICSI). 2013. Prevention and management of obesity for children and adolescents. Bloomington: Institute for Clinical Systems Improvement; 2013 [Accessed on 31 January 2018]. p. 41. Available at: /_asset/tn5cd5/ObesityChildhood.pdf.

International Union for Conservation of Nature (IUCN). 2015. Numbers of threatened species by major groups of organisms (19962015) Documents /2015_2 _RL_ Stats_Table_1.pdf.

Jadav HR, Galib R, Harish CR, Kumar PP. 2016. Preliminary Pharmacognostical profile of Tuvaraka (Hydnocarpus laurifolia (Dennst) Sleummer.) seeds. Medical Journal of Dr. D.Y. Patil University 9(2), 219-223.

Jagadish NRN, Gopalkrishna B. 2008. Evaluation of analgesic activity of different extracts of Stachytarpheta indica L. (Vahl). Biomed 3(3-4), 229-233.

Jaiswal BS. 2012. Solanum torvum: A review of its traditional uses, phytochemistry and pharmacology. International Journal of Pharma and Bio Sciences 3(4), 104-111.

Kandakumar S, Sathya V, Manju V. 2014. Synthesis and characterization of silver nanoparticle using Hydnocarpus alpine, its application as a potent antimicrobial and antioxidant agent- A novel study. International journal of Chem Tech Research 6(11), 4770-6.

Kang JG, Park CY. 2012. Anti-obesity drugs: a review about their acts and safety,” Diabetes & Metabolism Journal 36(1), 13-25.

Kar A. 2007. Pharmacognosy and pharmaco biotechnology. 2nd edn. New Delhi: New Age International (P) Ltd.

Kareru PG, Keriko JM, Kenji GM, Gachanja AN. 2010. Anti-termite and antimicrobial properties of paint made from T. peruviana (pers.) Schum. oil extract. African Journal of Pharmacy and Pharmacology 4(2), L087- 089.

Karthikeyan R, Solaimuthu C, Balakrishnan N. 2013. A study of performance and emissions of diesel engine fuelled with neat diesel and neat Hydnocarpus pentandra biodiesel. IOSR Journal of Mechanical and Civil Engineering 10(2), 53-57.

Katalinic V, Milos M, Kulisic T, Jukic M. 2006. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemisry 94, 550-557.

Kaur S, Mondal P. 2014. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiology and Experimentation 1(1), 1-6.

Kimura K, Lee JH, Lee IS, Lee HS, Park KH, Chiba S. 2004. Two potent competitive inhibitors discriminating alpha-glucosidase family I from family II. Carbohydrate Research 339, 1035-40.

Kopelman PG. 2000. Obesity as a medical problem. Nature 404 (6778), 635-43.

Kumar S, Narwal S, Kumar V, Prakash O. 2011. α-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Review, Jan-Jun 5(9), 19-29. doi: 10.4103/0973-7847.79096

Lacroix CR, Gerrath JM, Posluszny U. 1990. The Developmental morphology of Leea guineensis. I. Vegetative Development. Botanical Gazette 151(2), 204-209. DOI: 10.1086/337819.

Lahlou M. 2007. Screening of natural products for drug discovery. Expert Opinion on Drug Discovery 2(5), 697-705. DOI: 10.1517/17460441.2.5.697.

Lanting MV, Palaypayon CM. 2002. Forest tree species with medicinal uses. DENR Recommends Vol. 11. Ecosystems Research and Development Bureau, Department of nvironment and Natural Resources, College, Laguna p24.

Latayada FS, Uy MM. 2016. Screening of the antioxidant properties of the leaf extracts of Philippine medicinal plants Ficus nota (Blanco) Merr., Metroxylon sagu Rottb., Mussaenda philippica A. Rich., Inocarpus fagifer, and Cinnamomum mercadoi Vidal. Bulletin of Environment, Pharmacology and Life Sciences 5, 18-24.

Lawag IL, Aguinaldo AM, Naheed S, Mosihuzzaman M. 2012. a-Glucosidase inhibitory activity of selected Philippine plants. Journal of Ethnopharmacology 144(1), 217-9.

Lawrence L, Menon S, Vincent S, Sivaram VP, Padikkala V. 2016. Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark. Journal of Ayurveda and Integrative Medicine 7, 78-82.

Lebovitz HE. 1997. α-Glucosidase inhibitors. Endocrinology and Metabolism Clinics of North America 26, 539-55.

Lee EM, Lee SS, Chung BY, Cho JY, Lee IC, Ahn SR. 2000. Pancreatic lipase inhibition by C-glycosidic flavones isolated from Eremochloa ophiuroides. Molecules 15, 8251-9.

Liew PM, Yong YK. 2016. Stachytarpheta jamaicensis (L.) Vahl: From traditional usage to pharmacological evidence. Evidence-Based Complementary Alternative Medicine. 7842340.

Liu H, Qiu N, Ding H, Yao R. 2008. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Research International 41(4), 363-370.

Luna B, Pharm D, Feinglos MN. 2001. Oral agents in the management of Type 2 Diabetes Mellitus. American Family Physician 63, 1747-56, 1759-80.

Lunagariya NA, Patel NK, Jagtap SC, Bhutani KH. 2014. Inhibitors of pancreatic lipase: state of the art and clinical perspectives. Experimental and Clinical Sciences (EXCLI J) 13, 897-921.

Lynn A, Collins A, Fuller Z, Hillman K, Ratcliffe B. 2006. Cruciferous vegetables and colo-rectal cancer. Proceedings of the Nutrition Society 65(1), 135-44.

Ma J, Liu SJ. 2006. Research progress of DNJ in mulberry twig Food Science Technology 9, 112- 114.

Macabeo APG, Lopez ADA, Schmidt S, Heilmann J, Dahse HM, Alejandro GJD. 2013. Antitubercular and cytotoxic constituents from Goniothalamus gitingensis. Natural Products 8(1), 41-45.

Maqsood M, Dildar Ahmed D, Atique I, Malik W. 2017. Lipase inhibitory activity of Lagenaria siceraria fruit as a strategy to treat obesity. Asian Pacific Journal of Tropical Medicine 10(3), 305-310.

Maurya S, Kushwaha A, Singh S, Singh G. 2014. An overview on antioxidative potential of honey from different flora and geographical origins. Indian Journal of Natural Products and Resources 5, 9-19.

Mazura MP, Susanti D, Rasadah MA. 2007. Anti-inflammatory action of components from Melastoma malabathricum. Pharmaceutical Biology 45(5), 372-375.

McDougall GJ, Kulkarni NN, Stewart D. 2009. Berry polyphenols inhibit pancreatic lipase activity in vitro. Food Chemistry 115, 193-199.

Moreno DA, Ilic N, Poulev A, Brasaemle DL, Fried SK, Raskin I. 2003. Inhibitory effects of grape seed extract on lipases. Nutrition 19, 876-879.

Nahak G, Kantasahu R. 2011. Free radical scavenging activity of rhizome of Costus speciosus (Koen). International Journal of Institute of Pharmacy and Life Sciences 1, 62-67.

Ndebia EJ, Kamga R, Nchunga-Anye Nkeh B. 2007. Analgesic and anti- inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae). African Journal of Traditional and Complementary Medicine 4(2), 240-244.

Ng M, Fleming T, Robinson M. 2014. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: A systematic analysis for the Global Burden of Disease Study 2013. The Lancet 384(9945), 766-781.

Oke JM, Hamburger MO. 2002. Screening of some Nigerian medicinal plants for antioxidant activity using 2, 2, Diphenyl-Picryl-Hydrazyl radical. African Journal of Biomedical Research 5, 77-79.

Oladunmoye MK, Kehinde FY. 2011. Ethno botanical survey of medicinal plants used in treating viral infections among Yoruba tribe of south west Nigeria. African Journal of Microbioly Research 5, 2991-3004.

Patil RK, Makari HK, Gurumurthy H. 2008. In vitro antimicrobial activity of ethanol extract of Thevetia peruviana, ejeafche. Biotechnology An Indian Journal 2(1), 5-7.

Perez KJ, Jose MA, Aranico E, Madamba RZ. 2015. Phytochemical and antibacterial properties of the ethanolic leaf extract of Merremia Peltata (L.) Merr. and Rubus sp. Advances in Environmental Biology 9(19), 50-56.

Perk J. 2017. Non-communicable diseases, a growing threat to global health. E-Journal of Cardiology Practice 15(14).

Peteros NP, Uy MM. 2010. Antioxidant and cytotoxic activities and phytochemical screening of four Philippine medicinal plants. Journal of Medicinal Plants Research 4(5), 407-414.

Pharmacology. 2009. Drug–drug interactions with an emphasis on drug metabolism and transport, principles and practice. Chapter 12, 303-325.

Playford RJ, Pither C, Gao R. 2013. Use of the α-glucosidase inhibitor acarbose in patients with ‘Middleton syndrome’: normal gastric anatomy but with accelerated gastric emptying causing postprandial reactive hypoglycemia and diarrhea, Canadian Journal of Gastroenterology 27, 403-404.

Poddar K, Kolge S, Bezman L, Mullin GE, Cheskin LJ. 2011. Nutraceutical supplements for weight loss: a systematic review. Nutrition Clinical Practice 26(5), 539-52.

Putera I, Shazura KA. 2010. Antimicrobial activity and cytotoxic effects of Stachytarpheta jamaicensis (L.) Vahl crude plant extracts [Master dissertation], Universiti Teknologi Malaysia.

Quisumbing E. 1978. Medicinal Plants of the Philippines, Katha Publishing, Manila.

Raga DD, Espiritu RA, Shen CC, Ragasa CY. 2011. A bioactive sesquiterpene from Bixa orellana. Journal of Natural Medicines 65(1), 206-211.

Ragasa CY, Macuha MR, De Los Reyes MM, Mandia EH, Van Altenas IA. 2016. Chemical constituents of Ficus septica Burm. F. International Journal of Pharmaceutical and Clinical Research 8(11), 1464-1469.

Ragasa CY, Tsai P, Shen CC. 2009. Terpenoids and sterols from the endemic and endangered Philippine trees, Ficus pseudopalma and Ficus ulmifolia. Philippine Journal of Science 138(2), 205-209.

Rajbhar N, Kumar A. 2014. Pharmacological importance of Thevetia peruviana. International Journal of Pharmaceutical and Chemical Sciences 3(1), 260-263.

Regional Development Plan (RDP). 2011. Providing for basic needs: health. Bicol Regional Development Plan 2011-2016 pp. 45.

Roberts DM, Southcott E, Potter JM, Roberts MS, Eddleston M, Buckley NA. 2006. Pharmacokinetics of digoxin cross reacting substances in patients with acute yellow oleander (T. peruviana) poisoning, including the effect of activated charcoal. Therapeutic Drug Monitoring 28(6), 784-792.

Ruma OC, Zipagang TB. 2015. Determination of secondary metabolites and antibacterial property of extract from the leaves of Stachytarpheta jamaicensis (L.) Vahl. Journal of Medicinal Plants and Studies 3(4), 79-8.

Saeed N, Khan MR, Shabbir M. 2012. Antioxidant activity, total phenolic and total flavonoids contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine 12, 221.

Sahu RK, Kar M, Routay R. 2013. DPPH free radical scavenging activity of some leafy vegetables used by Tribals of Odisha, India. Journal of Medicinal Plant Studies 1, 21-27.

Saroya AS. 2011. Herbalism, Phytochemistry and Ethnopharmacology. Enfield, New Hampshire: Science Publishers.

Schaich KM, Tian X, Xie J. 2015. Hurdles and pitfalls in measuring antioxidant efficacy: a critical evaluation of ABTS, DPPH, and ORAC assays. Journal of Functional Foods 14, 111-125.

Seyedan A, Alshawsh MA, Alshagga MA, Koosha S, Mohamed Z. 2015. Medicinal plants and their inhibitory activities against pancreatic lipase: A Review. Evidence-Based Complementary and Alternative Medicine 1-13.

Shibano M, Kakutani K, Taniguchi M. 2008. Antioxidant constituents in the dayflower (Commelina communis L.) and their α-glucosidase-inhibitory activity. Journal of Natural Medicine 62, 349-353.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods of Enzymology 299, 152-178.

Sivapriya M, Srinivas L. 2007. Isolation and purification of a novel antioxidant protein from the water extract of Sundakai (Solanum torvum) seeds. Food Chemistry 104, 510-517.

Sjostrom L, Rissanen A, Andersen T, Boldrin M, Golay A, Koppeschaar HPF, Krempf M. 1998. Randomised placebo-controlled trial of orlistat for weight loss and prevention of weight regain in obese patients. Lancet 352, 167-172.

Sravani P, Murali CM, Sadik BS, Soubia SN. 2011. Evaluation of antidiuretic activity of Gmelina arborea. International Journal of Advances in Pharmacological Research 2(4), 157-161.

Srinivasan GV, Sharanappa P, Leela NK, Sadashiva CT, Vijayan KK. 2009. Chemical composition and antimicrobial activity of Leea indica (Burm. F.) Merr flowers. Natural Product Radiance 8(5), 488-493.

Srivastava N, Chauhan AS, Sharma B. 2012. Isolation and characterization of some phytochemicals from Indian traditional plants. Biotechnology Research International, 1-8.

Straughan DW, Fentem JE, Balls M. 1996. Replacement alternative and complimentary in vitro methods in pharmaceutical research. Pages 1-13 in JV Castel and MJG Lechon, Eds. In vitro methods in pharmaceutical research. Academic press.

Sulaiman CT, Balachandran I. 2012. Total phenolics and total flavonoids in selected Indian medicinal plants. Indian Journal of Pharmaceutical Science 74(3), 258-260.

Sulaiman MR, Zakaria ZA, Chiong HS, Lai SK, Israf DA, Azam Shah TM. 2009. Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae) in experimental animal models. Medical Principles and Practice 18(4), 272-279.

Tabopda TK, Ngoupayo J, Awoussong PK. 2008. Triprenylated flavonoids from Dorstenia psilurus and their α-glucosidase inhibition properties. Journal of Natural Products 71, 2068-2072.

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne D. 2006. Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating the antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis 19, 669-675.

Thomas RP, Thomas M, Paul J, Mohan M. 2013. Antifungal activity of Verbenaceae. Biosciences Biotechnology Research Asia 10(1), 355-360.

Tiwari BK, Brunton NP, Brennan CS. 2013. Handbook of Plant Food Phytochemicals: Sources, Stability and Extraction. Wiley-Blackwell. ISBN: 978-1-444-33810-2.

Torres RC, Sison FM, Israel MC. 2003. Phytochemical screening and biological studies on the crude methanol extract of Cinnamomum mercadoi, Vidal. Philippine Journal of Science 132(2), 27-32.

Varghese B, Sandhya S, Kavitha MP, Krishnakumar K. 2016. Genus Hydnocarpus: A Review. International Journal of Phytopharmacology 7(3), 143-154.

Vital PG, Velasco, RNJ, Demigillo JM, Rivera WL. 2010. Antimicrobial activity, cytotoxicity and phytochemical screening of Ficus septica Burm and Sterculia foetida L. leaf extracts. Journal of Medicinal Plants Research 4(1), 58-63.

Weigle DS. 2003. Pharmacological therapy of obesity: past, present, and future. Journal of Clinical Endocrinoly and Metabolism 88(6), 2462-2469.

Yahia EM (ed.) 2018. Fruit and Vegetable Phytochemicals: Chemistry and Human Health. 2nd Editio. John Wiley & Sons.

Yin Z, Zhang W, Feng F, Zhang Y, Kang W. 2014. α-Glucosidase inhibitors isolated from medicinal plants. Food Science and Human Wellness 3, 136-174.

Yun JW. 2011. Possible anti-obesity therapeutics from nature – A review. Phytochemistry 71, 1625-41.

Lilibeth A Cajuday, Daile Meek S Membreve, Michael V Montealegre, Jocelyn E Serrano, Diomerl Edward Baldo.
In vitro antidiabetic, antiobesity and antioxidant activities of selected endemic plants from Mount Mayon and Mount Malinao Albay, Philippines.
Int. J. Biosci. 16(3), 648-661, March 2020.
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