In vitro antidiabetic, antiobesity and antioxidant activities of selected endemic plants from Mount Mayon and Mount Malinao Albay, Philippines

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In vitro antidiabetic, antiobesity and antioxidant activities of selected endemic plants from Mount Mayon and Mount Malinao Albay, Philippines

Lilibeth A Cajuday, Daile Meek S Membreve, Michael V Montealegre, Jocelyn E Serrano, Diomerl Edward Baldo
Int. J. Biosci.16( 3), 635-648, March 2020.
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The present study investigated the in vitro biological activities of the ethanol extracts of thirteen endemic medicinal plants in Albay against diabetes, obesity and as free radical scavenger. Alpha-glucosidase (AG), porcine pancreatic lipase (PPL) inhibition tests and DPPH radical scavenging assay were used to determine the antidiabetic, antiobesity and antioxidant properties of the extracts. Quantitative phytochemical analysis of the extracts was performed using standard methods. Of the 13 endemics, 6 plants exhibited potent inhibitory activity (>70%) against AG enzyme. Hydnocarpus alcalae, Merremia peltata, Trema orientalis, Cascabela thevetia, Stachytarpheta jamaicencis, and Ficus septica. S. jamaicensis has the lowest computed IC50 (0.8ug/ml) indicating highest AG inhibitory activity compared to the other plants and the drug Acarbose (1.88ug/ml). A total of 7 plants were found to have strong inhibitory activity of >70% against PPL: Leea guineensis, Solanum torvum, Cheilocostus speciosus, Melastoma malabathricum, Dendrocnide meyeniana, Cinnamomum mercadoi, and Gmelina arborea. L. guineensis which recorded the highest activity against PPL, contains very high amount of alkaloids (237.49mg/g AE), flavonoids (490.54mg/g QE), and phenols (220.59mg/g GAE) and also exhibited strong antioxidant activity in the DPPH assay. M. malabathricum has the lowest IC50 suggesting that it has the highest activity against PPL compared to the other plants and the drug Orlistat (1.81ug/ml). DPPH radical scavenging assay showed that all plant extracts possessed statistically (p≤0.05) higher antioxidant activity against ascorbic acid suggesting promising potent source of antioxidants. There is a need to conduct further study on the toxicity of the evaluated plants.


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