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Biocontrol of black pod disease in Côte d’Ivoire through the selection of cocoa tree (Theobroma cacao L.) endophytic bacteria antagonist of Phytophthora spp.

By: Ouattara Adama, Coulibaly Klotioloma, Konate Ibrahim, Gogbe Françoise, N’guessan Walet Pierre, Acka Kotaix, Kouame Norbert, Tahi Mathias, Guiraud Brigitte, Assi Maryse, Kone Daouda, N’guessan François, Tidou Abiba Sanogo, Abdelkarim Filali-Maltouf

Key Words: Theobroma cacao L., Black pod disease, Phytophthora, Biocontrol, Endophytic bacteria

Int. J. Micro. Myco. 14(1), 1-10, March 2022.

Certification: ijmm 2022 0040 [Generate Certificate]

Abstract

This study aimed at selecting cocoa tree endophytic bacteria antagonistic to Phytophthora spp. in view to produce a new biofungicide capable of controlling black pod disease. Endophytic bacteria were isolated from healthy organs (roots, leaves and stems) of young nurseries of two clones NA32 and P7. These isolates were confronted in vitro with two Phytophthora species (Phytophthora palmivora and Phytophthora megakarya). Leaf and detached pod tests were carried out in a four-factor split-plot randomized experimental design. At total, 116 endophytic bacteria were isolated. These bacteria inhibited the radial growth of Phytophthora by 25.3±1.5 to 70.54±2.14%. Four isolates 48P, 60P, 23P and 18N were more effective in in vitro tests. The susceptibility index of the clone NA32 was reduced from 3.0 to 0.97 on leaf discs and from 7.57 to 1.27 on detached pods. These endophytic bacteria induced resistance to clone NA32 and increased the intrinsic resistance of clones PA150 and SCA6. Endophytic bacteria can be used for biocontrol of black pod disease. However, field trials are needed to confirm the stability of these laboratory results.

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Biocontrol of black pod disease in Côte d’Ivoire through the selection of cocoa tree (Theobroma cacao L.) endophytic bacteria antagonist of Phytophthora spp.

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Ouattara Adama, Coulibaly Klotioloma, Konate Ibrahim, Gogbe Françoise, N’guessan Walet Pierre, Acka Kotaix, Kouame Norbert, Tahi Mathias, Guiraud Brigitte, Assi Maryse, Kone Daouda, N’guessan François, Tidou Abiba Sanogo, Abdelkarim Filali-Maltouf.
Biocontrol of black pod disease in Côte d’Ivoire through the selection of cocoa tree (Theobroma cacao L.) endophytic bacteria antagonist of Phytophthora spp..
Int. J. Micro. Myco. 14(1), 1-10, March 2022.
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