Impact of high stocking density on growth parameters, amino acid profile and fatty acid profile of different fish species: a review

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Impact of high stocking density on growth parameters, amino acid profile and fatty acid profile of different fish species: a review

Shafaq Fatima, Razia Liaqat, Ramsha, Rida Mairaj, Shahida Ameen, Iqra Shahzadi
Int. J. Biosci.17( 6), 40-53, December 2020.
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Stocking density of fish depicts the number of fish that are stocked supplied at first per unit area. It is one of the most significant factors in determining the production of a fish farm. The stocking fish at low density results in high production costs, low economic gain and inadequate utilization of space. Increasing stocking density may activate stress response which negatively affects growth parameters, survival, behavior, health, feeding of fish. Different metabolic pathways related to lipid, carbohydrate and protein metabolism are also affected by this stress. So, for good yield fish should be stocked at optimum level. Optimum stocking density is the level where the maximum yields are reached. Fish are considered as a potential source of animal protein and essential nutrients in human diet. Amino acids, the building blocks of protein, act as a precursor of many enzymes, hormones, neurotransmitters, nucleic acids and other molecules essential for life. High stocking density affects amino acid profile of fish. The enzymatic activities and synthetic rates of functional proteins that is associated with responses of stress, HSD that is used as a stressor may influence amino acid requirements. High stocking density significantly affects water quality, growth parameters and amino acid profile of fish and it may induce stress in fish and may have negative impact on fatty acid profile if environmental conditions are not suitable for fish.


Abdel-Tawwab M, Hagras AE, Elbaghdady HAM, Monier MN. 2014. Dissolved oxygen level and stocking density effects on growth, feed utilization, physiology, and innate immunity of Nile Tilapia, Oreochromis niloticus. Journal of Applied Aquaculture 26(4), 340-355. https://doi/abs/10.1080/10454438.2014.959830

AFoss A, Vollen T, Qiestad V. 2003. Growth and oxygen consumption in normal and O2 supersaturated water, and interactive effects of O2 saturation and ammonia on growth in spotted wolffish (Anarhichas minor Olafsen). Aquaculture 22, 105–116.

Aidos L, Vasconi M, Abbate F, Valente LM, Lanfranchi M, Di Giancamillo A. 2019. Effects of stocking density on reared Siberian sturgeon (Acipenser baerii) larval growth, muscle development and fatty acids composition in a recirculating aquaculture system. Aquaculture Research 50(2), 588-598.

Aidos L, Vasconi M, Lanfranchi M, Di Giancamillo A. 2018. Effect of different stocking densities on growth, muscle development and fatty acid profile of Acipenser baerii larvae. International Journal of Health, Animal Science and Food Safety 5(1s),

Aijun M, Chao C, Jilin L, Siqing C, Zhimeng Z, Yingeng W. 2006. Turbot Scophthalmus maximus: stocking density on growth, pigmentation and feed conversion. Chinese Journal of Oceanology and Limnology 24(3), 307-312.

Aksnes A, Mundheim H, Toppe J, Albrektsen S. 2008. The effect of dietary hydroxyproline supplementation on salmon (Salmo salar L.) fed high plant protein diets. Aquaculture 275, 242– 24.

Aksungu M, Aksungur N. 2007. Effects of Stocking Density on Growth Performance, Survival and Food Conversion Ratio of Turbot (Psetta maxima) in the Net Cages on the southeastern coast of the Black Sea. Turkish Journal of Fisheries and Aquatic Sciences 7(2), 147-152.

Alhassan EH, Abarike ED, Ayisi CL. 2012. Effects of stocking density on the growth and survival of Oreochromis niloticus cultured in hapas in a concrete tank. African journal of agricultural research 7, 2405-2411.

Aragao C, Corte-Real J, Costas B, Dinis MT, Conceição LEC. 2008. Stress response and changes in amino acid requirements in Senegalese sole (Solea senegalensis Kaup 1858). Amino Acids 34(1), 143-148.

Asase AMOS. 2013. Effects of stocking density on the production of Nile tilapia (Oreochromis niloticus) in floating net cages on the Volta Lake (Doctoral dissertation, University of Ghana).

Ayyat MS, HI El-Marakby, SM Sharaf. 2011. Effect of dietary protein level, stocking density, and dietary pantothenic acid supplementation rate on performance and blood components of Nile tilapia Oreochromis niloticus. Journal of Applied Aquaculture 23, 122–135.

Baskerville-Bridges B, LJ Kling. 2000. Larval culture of Atlantic cod (Gadus morhua) at high stocking densities. Aquaculture 181, 61-69.

Belghit I, Skiba-Cassy S, Geurden I, Dias K, Surget A, Kaushik S, Seiliez I. 2014. Dietary methionine availability affects the main factors involved in muscle protein turnover in rainbow trout (Oncorhynchus mykiss). British journal of nutrition 112(4), 493-503.

Brown JA, Shahidi F. 1995. Lipid content and fatty acid composition of Arctic Charr (Salvelinus alpinus L.) reared at different stocking densities. Journal of Food Lipids 2(4), 269-286.

Buentello JA, Gatlin DM. 2001. Effects of elevated dietary arginine on resistance of channel catfish to exposure to Edwardsiella ictaluri. Journal of Aquatic Animal Health 13(3), 194-201.<0194:EOEDAO>2.0.CO;2

Charlton M. 2006. Branched-chain amino acid enriched supplements as therapy for liver disease. The Journal of nutrition 136(1), 295S-298S.

Chen C, Sander JE, Dale NM. 2003. The effect of dietary lysine deficiency on the immune response to Newcastle disease vaccination in chickens. Avian Diseases 47(4), 1346–1351.

Cheng Z, Buentello A, Gatlin DM. 2011. Effects of dietary arginine and glutamine on growth performance, immune responses and intestinal structure of red drum, Sciaenops ocellatus. Aquaculture 319(1-2), 247-252.

Costas B, Aragão C, Mancera JM, Dinis MT, Conceição LEC. 2007. High stocking density induces crowding stress and affects amino acid metabolism in Senegalese sole (Solea senegalensis Kaup). Aquaculture research 39, 1–9. https://doi/abs/10.1111/j.1365-2109.2007.01845.

Costas B, Conceição LE, Dias J, Novoa B, Figueras A, Afonso A. 2011. Dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of Senegalese sole (Solea senegalensis Kaup, 1858). Fish & shellfish immunology 31(6), 838-847.

Costas B, Conceição LE, Dias J, Novoa B, Figueras A, Afonso A. 2011. Dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of Senegalese sole (Solea senegalensis Kaup, 1858). Fish & shellfish immunology 31(6), 838-847.

Damasceno-Oliveira A, Fernandez-Duran B, Goncalves J, Serrao P, Soares-da-Silva P, Reis-Henriques MA, Coimbra J. 2007. Effects of cyclic hydrostatic pressure on the brain biogenic amines concentrations in the flounder, Platichthys flesus. General and Comparative Endocrinology 153, 385–389.

De Bandt,JP, Cynober L. 2006. Therapeutic use of branched-chain amino acids in burn, trauma, and sepsis. The Journal of nutrition 136(1), 308S-313S.

El‐Sayed AFM. 2002. Effects of stocking density and feeding levels on growth and feed efficiency of Nile tilapia (Oreochromis niloticus L.) fry. Aquaculture research 33(8), 621-626.

Espe M, Holen E. 2013. Taurine attenuates apoptosis in primary liver cells isolated from Atlantic salmon (Salmo salar). British Journal of Nutrition 110(1), 20-28.

Etzel MR. 2004. Manufacture and use of dairy protein fractions. The Journal of Nutrition 134(4), 996S-1002S.

Frechette MP, Bergeron P, Gagnon. 1996. on the use of self-thinning relationships in stocking experiments. Aquaculture 145, 91-112.

Gaylord TG, Barrows FT, Teague AM, Johansen KA, Overturf KE, Shepherd B. 2007. Supplementation of taurine and methionine to all-plant protein diets for rainbow trout (Oncorhynchus mykiss). Aquaculture 269(1-4), 514-524.

Gomes LGB, Baldisserotto JA, Senhorini. 2000. Effects of stocking density on water quality, survival, and growth of larvae of the matrinxa, Brycon cephalus (Characidae), in ponds. Aquaculture 183, 73-81.

Harpaz S. 2005. l-Carnitine and its attributed functions in fish culture and nutrition. Aquaculture 249(1-4), 3-21.

Herrera LC. 2015. The effect of stocking density on growth rate, Survival and yield of gift tilapia (Oreochromis niloticus) in Cuba: case study fish farm la juventud. United Nations University Fisheries Training Programme, Iceland [final project].

Hilge VW Steffens. 1996. Aquaculture of fry and fingerling of pike perch (Stizostedion lucloperca L.)–A short review. Journal of Applied Ichthyology 12, 167-170.

Hou H, Li B, Zhao X. 2011. Enzymatic hydrolysis of defatted mackerel protein with low bitter taste. Journal of Ocean University of China 10(1), 85-92.

Hyland K. 2007. Inherited disorders affecting dopamine and serotonin: critical neurotransmitters derived from aromatic amino acids. The Journal of nutrition 137(6), 1568S-1572S.

Jarwar AMA, 2008. A status overview of fisheries and aquaculture development in Pakistan with context to other Asian countries. Aquculture Asia 13(2), 13-18.

Jin Y, Tian LX, Zeng SL, Xie SW, Yang HJ, Liang GY, Liu YJ. 2013. Dietary lipid requirement on non-specific immune responses in juvenile grass carp (Ctenopharyngodon idella). Fish and Shellfish Immunology 34(5), 1202–1208.

Kilambi RV, Robison WR. 1979. Effects of temperature and stocking density on food consumption and growth of grass carp Ctenopharyngodon idella, Val. Journal of Fish Biology 15(3), 337-342.

Kohli MPS, Ayyappan S, Langer RK, Dubey K, Prakash C, Reddy AK,  Deshmukhe, G. 2002. Cage culture of carps, Labeo rohita and Cyprinus carpio at Powai lake, Mumbai, Maharashtra. Appl Fish Aquac 2, 1-4.

Li P, Gatlin DM. 2006. Nucleotide nutrition in fish: current knowledge and future applications. Aquaculture 251, 141–152.

Li P, Mai K, Trushenski J, Wu G. 2009. New developments in fish amino acid nutrition towards functional and environmentally oriented aquafeeds. Amino acids 37, 43-53.

Mañanos EL, Anglade I, Chyb J, Saligaut C, Breton B, Kah O. 1999. Involvement of γ-aminobutyric acid in the control of GtH-1 and GtH-2 secretion in male and female rainbow trout. Neuroendocrinology 69(4), 269-28.

Mane AM, Dube K, Varghese T, Chavan BR, Kamble MT. 2019. Effects of Stocking Density on Growth Performance, Survival and Production of Catla catla and Labeo rohita During Nursery Rearing in Cages. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 89(1), 275-2.

Mischoulon D, Fava M. 2002. Role of S-adenosyl-L-methionine in the treatment of depression: a review of the evidence. The American journal of clinical nutrition 76(5), 1158S-1161S.

Montero D, Izquierdo MS, Tort L, Robaina L, Vergara JM. 1999. High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream, Sparus aurata, juveniles. Fish Physiology and Biochemistry 20(1), 53-60.

Montero D, Robaina LE, Socorro J, Vergara JM, Tort L, Izquierdo MS. 2001. Alteration of liver and muscle fatty acid composition in gilthead seabream (Sparus aurata) juveniles held at high stocking density and fed an essential fatty acid deficient diet. Fish Physiology and Biochemistry 24(1), 63-72.

Pohlenz C, Buentello A, Mwangi W, Gatlin DM. 2012. Arginine and glutamine supplementation to culture media improves the performance of various channel catfish immune cells. Fish & shellfish immunology 32(5), 762-768.

Rathore RM, Liaset B, Hevrøy EM, El‐Mowafi A, Espe M. 2010. Lysine limitation alters the storage pattern of protein, lipid and glycogen in on‐growing Atlantic salmon. Aquaculture research 41(11), e751-e759.

Remø SC, Hevrøy EM, Olsvik PA, Fontanillas R, Breck O, Waagbø R. 2014. Dietary histidine requirement to reduce the risk and severity of cataracts is higher than the requirement for growth in Atlantic salmon smolts, independently of the dietary lipid source. British Journal of Nutrition 111(10), 1759-1772.

Ridha MT. 2006. Comparative study of growth performance of three strains of Nile tilapia. O. niloticus L. at two stocking densities. Aquaculture Research 37, 172–179.

Sadhu N, Sharma SR, Joseph S. 2014. Chronic stress due to high stocking
density in open sea cage farming induces variation in biochemical and immunological functions in Asian seabass (Lates calcarifer). Fish Physiology and Biochemistry 40, 1105–1113.

Sathivel S, Smiley S, Prinyawiwatkul W, Bechtel PJ. 2005. Functional and nutritional properties of red salmon (Oncorhynchus nerka) enzymatic hydrolysates. Journal of Food Science 70(6), c401-c406. https://doi/abs/10.1111/j.13652621.2005.tb11437

Shamushaki VAJ, Kasumyan AO, Abedian A, Abtahi B. 2007. Behavioural responses of the Persian sturgeon (Acipenser persicus) juveniles to free amino acid solutions. Marine and Freshwater Behaviour and Physiology 40, 219–224.

Sharma J, Chakrabarti R. 2003. Role of stocking density on growth and survival of catla, Catla catla, and rohu, Labeo rohita, larvae and water quality in a recirculating system. Journal of applied aquaculture 14(1-2), 171-178.

Suresh AV, Lin CK.  1992. Effects of stocking density on water quality and production of red tilapia in recirculated water system. Aquacultural Engineering 11.

Tokach MD, Goodband RD, Nelssen JL. 1993. Valine: a limiting amino acid for high-producing lactating sows.

Tolussi CE, Hilsdorf AWS, Caneppele D, Moreira RG. 2010. The effects of stocking density in physiological parameters and growth of the endangered teleost species piabanha, Brycon insignis (Steindachner,1877). Aquaculture 310(1-2), 221-228.

Tucker CS, EH Robinson. 1990. Channel Catfish Farming Handbook. Van Nostrand Reinhold, New York, and New York.

Wallace JC, Kolbeinshavn AG. Reinsnes TG. 1988. The effects of stocking density on early growth in Arctic charr, Salvelinus alpinus (L.). Aquaculture 73, 101-110.

Wu G. 2010. Functional amino acids in growth, reproduction, and health. Advances in Nutrition 1(1), 31-37.

Yousefi M, Paktinat M, Mahmoudi N, Pérez-Jiménez A, Hoseini SM. 2016. Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress. Fish physiology and biochemistry 42(5), 1417-1425.

Zhao H, Soufan O, Xia J, Tang R, Li D. 2019. Transcriptome and physiological analysis reveal alterations in muscle metabolisms and immune responses of grass carp (Ctenopharyngodon idellus) cultured at different stocking densities. Aquaculture 503, 186-197.

Parpoura ACR, Alexis MN. 2001. Effects of different dietary oils in sea bases (Dicentrarchuslabrux) nutrition. Aquaculture International 9, 463-476.

Henderson RJ, Tocher DR. 1999. The lipid composition and biochemistry of freshwater fish. Progress in lipid research 26(4), 281-347.

Ackman RG. 1994. Seafood lipids. In: Seafoods: Chemistry, processing, technology and quality. Shahidi F, Botta JR (eds), Blackie Academic and Professional, London, p 34–48.

Kolakowska A. 2003. Lipid oxidation in food systems. Chemical and functional properties of food lipids, 133-165.

Gbogouri GA, Linder M, Fanni J, Parmentier M. 2006. Analysis of lipids extracted from salmon (Salmo salar) heads by commercial proteolytic enzymes. European Journal of Lipid Science and Technology 108(9), 766-775.

Frenoux JMR, Prost ED, Belleville JL, Prost JL. 2001. A polyunsaturated fatty acid diet lowers blood pressure and improves antioxidant status in spontaneously hypertensive rats. The Journal of nutrition 131(1), 39-45.