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Implications of prophetic factors and their potential role in the development of radicular cyst

Research Paper | February 1, 2017

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Arif Malik, Ayesha Zahid, Shahzad Ahmad, Sulayman Waquar, Muhammad Abdul Basit Ashraf

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Int. J. Biosci.10( 2), 201-209, February 2017

DOI: http://dx.doi.org/10.12692/ijb/10.2.201-209


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Radicular cyst (RC) is the most common endodontic and inflammatory disease encompasses damage of periradicular tissues and resorption of bone adjacent to root of exaggerated teeth. Inflammatory cytokines uphold cyst growth and bone resorption as they are responsible to modulate bone remodeling. Reactive oxygen species (ROS) and nitric oxide derived from macrophages after bacterial lipopolysaccharides (LPS) may enhance bone loss by boosting the cytokine induced matrix metalloproteinases (MMPs) production in osteoblasts. Forty samples of radicular cysts and twenty from normal pulp were taken from extracted teeth (As control). Glutathione (GSH), Glutathione reductase (GRx), Glutathione peroxidase (GPx), Catalase (CAT), Malondialdehyde (MDA), superoxide dismutase (SOD), Vitamin-C, Vitamin-E, Nitric oxide (NO), Lipopolysaccharides (LPS), advanced oxidation protein products (AOPPs), advanced glycation end products (AGEs) were estimated spectrophotometrically. Inducible nitric oxide synthase (iNOS) and matrix metalloproteinases (MMPs) activity was measured in supernatants by kit method from tissue homogenates. Significantly (<0.05) decreased levels of antioxidants (SOD, CAT, GSH, vitamin C, vitamin E, GPx and GRx) were observed in tissue homogenate of radicular cyst as compared to healthy controls. Higher levels of MDA, AOPPs, AGEs, NO, iNOS LPS and MMP-9 signifies their importance and role in the development/expansion of radicular cyst. Inducible nitric oxide synthase (iNOS) play a key role to stimulate and release of elevated nitric oxide levels in an environment provided by tumor necrosis factor alpha. Lipopolysaccharide directly activates the host cells to secrete MMPs which play a key role in degrading the bone matrix and hence expansion of radicular cyst.


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Implications of prophetic factors and their potential role in the development of radicular cyst

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