In vitro antibacterial activity of Fumaria indica (H.) pugsley and SilybummarianumL. against planktonic and biofilm form of Pseudomonas aeruginosa
Paper Details
In vitro antibacterial activity of Fumaria indica (H.) pugsley and SilybummarianumL. against planktonic and biofilm form of Pseudomonas aeruginosa
Abstract
Present study investigated antibacterial potential of aerial partsof Fumaria indica andSilybummarianum against planktonic and biofilm form of 3 clinical strains P1, P2 and P3 of Pseudomonas aeruginosa. Antibacterial activity against planktonic form was investigated by Well diffusion method. The antibiofilm activity was assessed by using Pellicle inhibition (PI) and Congo red assay (CR). Crude methanolic extracts of Fumaria indicashowed good antibacterial activity against P. aeruginosa with maximum 15.8mm zone of inhibition (ZOI) against P2 strain of P. aeruginosa and minimum 13.2mm zone of inhibition against P3 strain. Silybummarianumshowed maximum 13.3mm zone of inhibition and minimum 12.6mm ZOI against P2 and P3 strains of P. aeruginosa respectively.Furthermore, F. indica showed a moderate (+++) to weak (++++) antibiofilm activity against all tested strains of P. aeruginosa in Pellicle inhibition assay, while S. marianum possessed from moderate to no activity against tested bacterial strains, respectively. In Congo red assay F. indica showed a strong antibiofilm effect as compared to S. marianum. Based on these results it may be concluded that leaf extractof Fumaria indicapossessed a good to moderate antibacterial activityagainst planktonic and biofilm form of P. aeruginosa. While Silybummarianumpossessed moderate to weak antibacterial potential against P. aeruginosa.
Ahmad L, Semotiuk A, Zafar M, Ahmad M, Sultana S, Liu QR, Zada MP, Abidin SZ, Yaseen G. 2015. Ethnopharmacological documentation of medicinal plants used for hypertension among the local communities of Dir lower, Pakistan. Journal of Ethnopharmacology 175, 138–146. http://dx.doi.org/10.1016/j.jep.2015.09.014.
Ahmad I, Ibrar M, Barkatullah Ali N. 2011. Ethnobotanical Study of Tehsil Kabal, Swat District, KPK, Pakistan. Journal of Botany 2011, 9. http://dx.doi.org/10.1155/2011/368.572
Begun J, Gaiani JM, Rohde H, Mack D, Calderwood SB, Ausubel FM, Sifri CD. 2007. Staphylococcal biofilm exopolysaccharide protects against Caenorhabditiselegans immune defenses. PLoS Pathogens 3(4), e57.
Bisset NG. 1994. Herbal drugs and phytopharmaceuticals. Boca Raton, FL, CRC Press. A handbook for practice on a scientific basis. Medpharm Scientific Publishers, Stuttgart and CRC Press,Boca Raton, Ann Arbor, London, Tokyo. ISBN: 3, 88763-025 4.
Cegelski L, Marshall GR, Eldridge GR, Hultgren SJ. 2008. The biology and future prospects of antivirulence therapies. Nature Reviews Microbiology 6(1), 17–27. http://dx.doi:10.1038/nrmicro1818.
Cerca N, Martins S, Pier GB, Oliveira R, Azeredo J. 2005. The relationship between inhibition of bacterial adhesion to a solid surface by sub-MICs of antibiotics and subsequent development of a biofilm. Res Microbiol 156, 650-655 http://dx.doi:10.1016/jresmic.2005.02.004.
Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280, 295–298. http://dx.doi:10.1126/Science.280.5361.295.
Deep A, Chaudhary U, Gupta V. 2011. Quorum sensing and bacterial pathogenicity: from molecules to disease. Journal ofLaboratory Physicians 3(1), 4– 11. http://dx.doi:10.4103/0974-2727.78553.
Cragg GM and Newman DJ. 2002. Drugs from Nature: Past Achievements, Future Prospects. In: M. M. Iwu and J. C. Wootton, Eds., Ethnomedicine and Drug Discovery, Elsevier Science, Amsterdam, 2002, 23-37 p.
Evren E, Yurtcu E. 2015. In vitro effects on biofilm viability and antibacterial and antiadherent activities of silymarin. Folia Microbiologica 60(4), 351-6. http://dx.doi:10.1007/s12223-015-0399-6.
Furneri P, Garozzo A, Musumarra M, Scuderi A, Russo A, Bonfiglio G. 2003. Effects on adhesiveness and hydrophobicity of sub-inhibitory concentrations of netilmicin. Int J Antimicrob Agents 22, 164–167. http://dx.doi:10.1016/S0924-8579-(03)-00149-3.
Greenlee H, Abascal K, Yarnell E and Ladas E. 2007. Clinical Applications of Silybummarianum in oncology. Integrative Cancer Therapies. 6(2): 158– 65. http://dx.doi.org/10.1177/1534735407301727
Hall-Stoodley L, Costerton JW, Stoodley P. 2004. Bacterial biofilms: From the natural environment to infectious diseases. Nat. Rev 2, 95– 108. http://dx.doi:10.1038/nrmicro821.
Hoiby N, Ciofu O, Bjarnsholt T. 2010. Pseudomonas aeruginosabiofilms in cystic fibrosis. Future Microbiology 5(11), 1663–1674. http://dx.doi:10.2217/fmb.10.125
Joshua GWP, Guthrie-Irons C. Karlyshev AV, Wren BW. 2006. Biofilm formation in Campylobacter jejuni. Microbiology 152, 387-396. http://dx.doi:10.2217/fmb.10.125.
Kang CG, Hah DS, Kim CH, Kim YH, Kim S, Kim JS. 2011. Evaluation of Antimicrobial Activity of the Methanol Extracts from 8 Traditional Medicinal Plants. Toxicology Research. 27(1), 31-36. http://dx.doi:10.5487/TR.2011.27.1.031.
Karatan E, Watnick P. 2004. Signals, regulatory networks, and materials that build and break bacterial biofilms. Microbiology and Molecular Biology Reviews 73, 310–347. http://dx.doi:10.1099/mic.0.28358.
Khan A, Tak H, Nazir R, Lone BA, Parray JA. 2014. In vitro anthelmintic and antimicrobial activities of methanolic extracts of Fumaria indica. Clinical Microbiology 3, 161. http://dx.doi.org/10.4172/2327.5073.1000161.
Kim HS, Park HD. 2013. Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14. PLoS ONE. 8(9), http://dx.doi.org/10.1371
Kroll DJ, Shaw HS, Oberlies NH. 2007. Milk Thistle Nomenclature: Why It Matters in Cancer Research and Pharmacokinetic Studies. Integrative Cancer Therapies 6(2), 110–9. http://dx.doi.org/10.1177/1534735407301825.
Merritt JH, Brothers K.M Kuchma SL, O’Toole GA. 2007. SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function. Jouranl of Bacteriology 189, 8154–8164. http://dx.doi:10.1128/JB.00585-07.
Pandey MB, Singh AK, Singh JP, Singh VP, Pandey VB. 2008. Fuyuziphine, a new alkaloid from Fumaria indica. Natural Product Research 22(6), 533-6. http://dx.doi:10.1080/14786410701592596.
Pattiyathanee P, Vilaichone RK, Chaichanawongsaroj N. 2009. Effect of curcumin on Helicobacter pylori biofilm formation. African Journal of Biotechnology 8(19), 5106-5115.
Parekh J, Karathia N, Chanda S. 2006. Screening of Some Traditionally Used Medicinal Plants for Potential Antibacterial Activity. Indian Journal of Pharmaceutical Science 68, (832-834). http://dx.doi:10.4103/0250-474X.31031.
Rathi A, Srivastava AK, Shirwaikar A, Rawat, AKS, Mehrotra S. 2008. Hepatoprotective potential of Fumaria indicaPugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine 15(6-7), 470-7. http://dx.doi:10.1016/j.phymed.2007.11.010.
Sandasi M, Leonard CM, Viljeon AM. 2009. The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes. Letters in Applied Microbiology 50(1), 30–35. http://dx.doi:10.1111/j.1472-765X.2009.02747.X.
Walter C, Shinwari ZK, Afzal I, Malik RN. 2011. Antibacterial activity in herbal products used in Pakistan. Pakistan Journal of Botany 43, 155-162.
Imran Khan, Azhar Hussain Shah, Wajiha Khan, Uzma Khan, 2016. In vitro antibacterial activity of Fumaria indica (H.) pugsley and SilybummarianumL. against planktonic and biofilm form of Pseudomonas aeruginosa. Int. J. Biosci., 8(4), 94-102.
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