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Research Paper | July 1, 2013

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In vitro antibacterial effect of Tinospora cordifolia extracts against some selective bacterial pathogens

Md. Shamim Hossain, Mst. Touhida khatun1, Md. Mehedi Hassan

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Int. J. Biosci.3(7), 156-161, July 2013

DOI: http://dx.doi.org/10.12692/ijb/3.7.156-161

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Abstract

The petroleum spirit, methanol, dichloromethane and ethyl acetate extracts of the leaves of Tinospora cordifolia (Menispermaceae) were screened for their anti-microbial activity using disc-diffusion method on nutrient agar medium. This plant was tested against four bacteria; two Gram-positive bacteria (Bacillus subtilis and Sarcina lutea) and two Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae).All the organic solvent extract showed susceptibility against Sarcina lutea, E.coli and Bacillus subtilis whereas Klebsiella pneumoniae showed resistant against all the organic solvent extract. It was found that the antimicrobial activity of the methanol extract (11mm) showed the maximum zone of inhibition against Escherichia coli. On the other hand petroleum spirit extract showed the maximum inhibition against Bacillus subtilis (3mm) respectively. The minimum inhibitory concentration for petroleum spirit and dichloromethane extracts of Tinospora cordifolia were ranged between 32-512µg/mL for tested bacteria. The result of this study demonstrates the potentiality of Tinospora cordifolia as a source of antimicrobials that could be harness for use in the health care delivery process.

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In vitro antibacterial effect of Tinospora cordifolia extracts against some selective bacterial pathogens

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