In vitro Antioxidant activity of stem and leaves extracts of Hedera nepalensis K. Koch
Paper Details
In vitro Antioxidant activity of stem and leaves extracts of Hedera nepalensis K. Koch
Abstract
Two in vitro methods viz 1,1-diphenyl-2-picrylhydazyl free radical scavenging assay and hydrogen peroxide scavenging assay were used for the antioxidant activity of the Hedera nepalensis leaves and stem methanol, ethanol, ethyl acetate, dichloromethane, petroleum ether extracts. The 1, 1-diphenyl-2-picrylhydazyl scavenging activity was studied at the absorbance of 517nm. The absorbance was due the number of the electrons taken up. At increase in concentration absorbance was reduced gradually. Concentration of 0.1 mg/ml exhibited 42.72, 26.36, 46.36, 58.18 and 51.81 scavenging activity for leaves extracts respectively. Similarly concentration of 0.1 mg/ml exhibited 34.02, 45.13, 31, 53, and 61 scavenging activity for stem extracts respectively. However, DPPH scavenging activity of ascorbic acid at this concentration exhibited marked scavenging activity of 72.72 and 76 respectively.The hydrogen peroxide scavenging activity was studied at the absorbance of 285nm with average time of 10 minutes incubationwas studiedfor leaves methanol, ethanol, ethyl acetate, dichloromethane, petroleum ether extracts. Concentration of 0.1 mg/ml exhibited 50, 54.54, 47.27, 60, and 70.90 hydrogen peroxide scavenging activityfor leaves extracts respectively. Similarly concentration of 0.1 mg/ml exhibited 50, 77, 74, 20.66 and 72 hydrogen peroxide scavenging activity for stem extractsrespectively. However, hydrogen peroxide scavenging activity of ascorbic acid at this concentration exhibited marked scavenging activity of 80.50 and 85. The results conclude that the extracts of the H. neplalensis has the potential of antioxidant and be a natural source to treat pathological diseases.
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