In-vitro antioxidant and anti-microbial potential of the pollen extracts of Pinus roxburghii Sarg
Paper Details
In-vitro antioxidant and anti-microbial potential of the pollen extracts of Pinus roxburghii Sarg
Abstract
Pollen grains of Pinus roxburghii Sarg. Possess antioxidant and antibacterial potential. To test this hypothesis, pollen dust was collected from the P. roxburghii trees of the University of Malakand, Dir Lower Campus and its methanolic crude extracts at various concentrations (i.e. 1000, 500, 250, 125 and 62.50 μg/mL) were prepared and tested for antioxidant activity using DPPH and ABTS methods. The possible antimicrobial potential of the pollens was also tested using Ager Disc Diffusion method against seven different pathogenic bacterial strains i.e. Proteus mirabilis, Staphylococcus aurous, Escherichia coli, Bacillus cereus, Salmonella typhi, Klebsiella pneumonia and Pseudomonas aeruginosa. Results obtained for antioxidant activity show that in the free radical scavenging activity the DPPH value for 1000μg/mL was the highest with the value of 81.70±0.74, While the ABTS value was 78.30±0.15 for the same concentration. The IC50 values of the pollen extract of the ABTS was 23.46 µg/mL while the DPPH was 15.98µg/mL. The most sensitive bacteria recorded in the antimicrobial activity for the concentration of 100µg/mL of the pollen extract was Pseudomonas aeruginosa (8.666± 0.71) using the mean± standard deviation value as a measure followed by Bacillus cereus(5.666± 4.24), Escherichia coli (5.333± 0.71) and Staphylococcus aurous (4.333± 0.71). The ANOVA results obtained for the 50µg/mL of methanolic pollen extract in comparison with the controlled 50mg/mL of antibiotic (Ciprofloxacin),show a significant difference between their action of inhibition with the calculated P value being much smaller (i.e. 0.00) than the alpha level of 0.5. We conclude by reporting it for the first time that P. roxburghii pollens have antioxidant and antibacterial potential and can possibly be exploited as a food and medicine source.
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