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In vitro assessment and comparative efficacy on antioxidative effects of petroleum ether and methanol extract of different citrus fruit peels in Bangladesh

Md. Maniruzzaman, Ashik Mosaddik, Simin Shabnam Lopa, Md. Mahadi Hasan, Ashraful Mahmud Tuhin, Md. Abu Zubair, Md. Shahidul Haque

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Int. J. Biosci.16(3), 321-336, March 2020

DOI: http://dx.doi.org/10.12692/ijb/16.3.321-336


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The prevention of oxidative stress caused by microorganisms or other adverse environmental stimuli is not clarified well.  The current study has been undertaken regarding this phenomenon along with petroleum ether and methanol extraction of five varieties of citrus fruit peels, C. limittoids, C. hystrix, C. medica, C. reticulate and C. lemon. For total antioxidant capacity during petroleum ether extraction, different concentrations of peel extract were used.  C. reticulate and C. medica were found to be potential showing higher antioxidant capacity and the absorbance were increased dose dependently when compared to catechin. Other varieties also showed a increased absorbance indicating higher antioxidative effects. Similarly, the extracts of peels after methanol treatment were found to have a higher absorbance where the absorbance was increased dose dependently and C. hystrix and C. limittoids showed potent effect.  Other varieties were shown to have increased antioxidative effects because of their increasing absorbance.  The reducing power capacity during petroleum ether extraction was enhanced dose dependently while C. reticulate and C. medica showed higher absorbance showing higher reducing power capacity.  The absorbance of different concentrations of extract in methanol was examined and the values were increased similarly with increasing concentrations.  C. reticulate and C. medica showed higher effect when compared to ascorbic acid.  Other varieties also showed enhanced effect and the effects were assumed to be potential in both petroleum ether and methanol extract.  Therefore, all five varieties are recognized to have bioactive compounds with diverse clinical importance in response to adverse biotic or abiotic stress.


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In vitro assessment and comparative efficacy on antioxidative effects of petroleum ether and methanol extract of different citrus fruit peels in Bangladesh

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