In-vitro assessment of anti-bacterial, anti-fungal and anti-oxidant potential of Ficus carica stems bark

Paper Details

Research Paper 01/01/2019
Views (374) Download (8)

In-vitro assessment of anti-bacterial, anti-fungal and anti-oxidant potential of Ficus carica stems bark

Farooq Azam, Sabi-Ur-Rehman, Hafiz Ahmad Hamad, Anwar Khalid, Abdul Samad
Int. J. Biosci.14( 1), 520-524, January 2019.
Certificate: IJB 2019 [Generate Certificate]


Medicinal plants have been using for treatment of different diseases since ages. Ficus carica is a plant which is abundantly present and easily available in Pakistan. In this study methanolic extracts of Ficus carica stem bark was examined for anti-bacterial, anti-fungal and anti-oxidant potential. Anti-bacterial and anti-fungal potential was examined following disc diffusion method whereas anti-oxidant activity was determined using DPPH scavenging method. The maximum antibacterial activity was recorded 19 mm against Klebsiella pneumonae and Escherichia coli, and the highest anti-fungal potential was also recorded 19 mm against Fusarium oxysporum and Mucor racemosus each. The mean anti-oxidant activity was also recorded 63.45% in comparison to DPPH. The plant extracts was exhibited appropriate anti-oxidant potential and antimicrobial activity against various human pathogenic bacterial and fungal strains.


Ahad H, Sreeramulu J, Kumar C, Anuradha C, Reddy K, Sushma K, Savithri R. 2010. Fabrication and in-vitro permeation studies of indomethacin-Ficus carica fruit mucilage patches. International Journal of Applied Biology and Pharmaceutical Technology 1(3), 786-792.

Ahmed F, Siddesha JM, Urooj A, Vishwanath BS. 2010. Radical scavenging and angiotensin converting enzyme inhibitory activities of standardized extracts of Ficus racemosa stem bark. Phytotherapy research 24(12), 1839-1843.

Ao C, Li A, Elzaawely AA, Xuan TD, Tawata S. 2008. Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract. Food control 19(10), 940-948.

Aref HL, Salah K, Chaumont JP, Fekih A, Aouni M, Said K. 2010. In vitro antimicrobial activity of four Ficus carica latex fractions against resistant human pathogens (antimicrobial activity of Ficus carica latex). Pak Journal Pharm Science 23(1), 53-58.

Badgujar SB, Patel VV, Bandivdekar AH, Mahajan RT. 2014. Traditional uses, phytochemistry and pharmacology of Ficus carica: A review. Pharmaceutical Biology 52(11), 1487-1503.

Borris RP. 1996. Natural products research: perspectives from a major pharmaceutical company. Journal of Ethnopharmacology 51(1-3), 29-38.

Chaturvedi V, Springer DJ, Behr MJ, Ramani R, Li X, Peck MK, Samsonoff WA. 2010. Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with white nose syndrome (WNS). PLoS One 5(5), e10783.

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical microbiology reviews 12(4), 564-582.

Debib A, Tir‐Touil A, Mothana R, Meddah B, Sonnet P. 2014. Phenolic content, antioxidant and antimicrobial activities of two fruit varieties of Algerian Ficus carica L. Journal of food biochemistry 38(2), 207-215.

Dueñas M, Pérez-Alonso JJ, Santos-Buelga C, Escribano-Bailón T. 2008. Anthocyanin composition in fig (Ficus carica L.). Journal of Food Composition and Analysis 21(2), 107-115.

Jeong MR, Kim HY, Cha JD. 2009. Antimicrobial activity of methanol extract from Ficus carica leaves against oral bacteria. Journal of Bacteriology and Virology 39(2), 97-102.

Joshi S, Boyd S. 2009. Sensor selection via convex optimization. Institute of Electrical and Electronics Engineers Transactions on Signal Processing      57(2), 451-462.

Lee YS, Cha JD. 2010. Synergistic antibacterial activity of Fig (Ficus carica) leaves extract against clinical isolates of methicillin-resistant Staphylococcus aureus. korean journal of microbiol biotechnology 38(4), 405-413.

Mopuri R, Ganjayi M, Meriga B, Koorbanally NA, Islam MS. 2017. The effects of Ficus carica on the activity of enzymes related to metabolic syndrome. Journal of Food and Drug Analysis.

Nascimento GG, Locatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology 31(4), 247-256.

Nebedum J, Udeafor P, Okeke C. 2010. Comparative effects of ethanolic extracts of Ficus carica and Mucuna pruriens leaves on haematological parameters in albino rats. Biokemistri 22(2).

Neves JM, Matos C, Moutinho C, Queiroz G, Gomes LR. 2009. Ethnopharmacological notes about ancient uses of medicinal plants in Trás-os-Montes (northern of Portugal). Journal of Ethnopharmacology 124(2), 270-283.

Oyelana O, Durugbo E, Olukanni O, Ayodele E, Aikulola Z, Adewole A. 2011. Antimicrobial activity of Ficus leaf extracts on some fungal and bacterial pathogens of Dioscorea rotundata from Southwest Nigeria. Journal of Biological sciences 11(5), 359-366.

Parekh J, Chanda S. 2007. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research 10(2).

Ramazani A, Zakeri S, Sardari S, Khodakarim N, Djadidt ND. 2010. In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense. Malaria Journal 9(1), 124.

Shi J, Ross CR, Chengappa M, Sylte MJ, McVey DS, Blecha F. 1996. Antibacterial activity of a synthetic peptide (PR-26) derived from PR-39, a proline-arginine-rich neutrophil antimicrobial peptide. Antimicrobial agents and chemotherapy 40(1), 115-121.

Spellberg B, Powers JH, Brass EP, Miller LG, Edwards Jr JE. 2004. Trends in antimicrobial drug development: implications for the future. Clinical Infectious Diseases 38(9), 1279-1286.