In vitro controlling of selected human diarrhea causing bacteria by clove extracts (Syzygium aromaticum L.)

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Research Paper 01/08/2011
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In vitro controlling of selected human diarrhea causing bacteria by clove extracts (Syzygium aromaticum L.)

M Mostafizur Rahman, M Atikur Rahman, M Soriful Islam, M Firoz Alam
Int. J. Biomol. & Biomed.1( 2), 17-26, August 2011.
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Antibacterial activity of clove extracts (Syzygium aromaticum L.) was proven against five diarrhea causing bacteria. This was further confirmed when compared with commonly used three commercial antibiotics (ciprofloxacin, tetracycline and erythromycin) as a positive control. Significant differences (P<0.0001) were observed in the effect of the antimicrobial agents (clove extracts and antibiotics), and in the sensitivities of the bacterial species (P<0.0001) to the antimicrobial agents. Clove extracts had significant (P<0.001) activity with the acetone extract demonstrating highest activity followed by antibiotics and other extracts against tested bacteria. The zone of inhibition of clove extracts was ranged from 7.33 to 12.00 mm whereas in antibiotics, it was 0.00 to 11.67 mm. Of all the bacteria, Salmonella typhimurium was the most susceptible against all of the extracts as well as concentrations of clove, while low MIC (180 mgml-1) and MBC (680 mgml-1) of the extracts were observed against Shigella dysenteriae. Consequently, clove has a significant antidiarrheal activity and it could be used as an effective antibacterial agent, alternative to the use of antibiotics.


Agunu A, Yusuf S, Andrew GO, Zezi AU, Abdurahman EM. 2005. Evaluation of five medicinal plants used in diarrhoea treatment in Nigeria. Journal of Ethnopharmacology 101(1-3), 27.

Amstrong D, Cohen J. 1999. Infectious Diseases. Vol. 1, Section 2. Mosby, Spain, 35.1-35.70 Chapter, 35).

Bae EA, Han MJ, Kim NJ, Kim DH. 1998. Anti-Helicobacter pylori activity of herbal medicines. Biological and Pharmaceutical Bulletin 21(9), 990-1002.

Bansod S, Rai M. 2008. Antifungal activity of essential oils from Indian medicinal plants against Human pathogenic Aspergillus fumigatus and A. niger. World Journal of Medical Sciences 3(2), 81-88.

Burt SA, Reinders RD. 2003. Antibacterial activity of selected plant essential oils against Escherichia coli O157: H7. Letters in Applied Microbiology 36, 162-167.

Cai L, Wu CD. 1996. Compounds from Syzygium aromaticum processing growth inhibitory activity against oral pathogens. Journal of Natural Products 59(10), 987- 990.

Chaieb K, Hajlaoui H, Zmantar T, Kahla-Nakbi AB, Rouabhia M, Mahdouani K, Bakhrouf A. 2007b. The Chemical Composition and Biological Activity of Clove Essential Oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): A Short Review. Phytotherapy Research 21(6), 501-506.

Chaieb K, Zmantar T, Ksouri R, Hajlaoui H, Mahdouani K, Abdelly C, Bakhrouf A. 2007a. Antioxidant properties of essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. Mycoses 50(5), 403-406.

de Guzman CC, Siemonsma JS. 1999. Plant Resources of South-East Asia No. 13: Spices, (Bogor, Indonesia, Prosea Foundation),

De M, Krishna De A, Banerjee AB. 1999. Antimicrobial screening of some Indian spices. Phytotherapy Research 13(7), 616-618.

Dorman HJ, Deans SG. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88(2), 308-316.

Doughari JH, Elmahmood AM, Manzara S. 2007. Studies on the antibacterial activity of root extracts of Carica papaya L. African Journal of Microbiology Research 1(3), 037-041.

Eja ME, Asikong BE, Abriba C, Arikpo GE, Anwan EE, Enyi-Idoh KH. 2007. Comparative assessment of the antimicrobial effects of garlic (Allium sativum) and antibiotics on diarrheagenic organisms. The Southeast Asian Journal of Tropical Medicine and Public Health 38(2), 343-348.

Ekwenye UN, Elegalam NN. 2005. Antibacterial activity of ginger (Zingiber officinaly Roscoe) and garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. International Journal of Molecular Medicine and Advance Sciences 1(4), 411-417.

El-mahmood AM, Ameh JM. 2007. In vitro antibacterial activity of Parkia biglobosa (Jacq) root bark extract against some microorganisms associated with urinary infections. Africal Journal of Biotechnology 6(11), 1272-1275.

El-Mahmood AM, Doughari JH. 2008. Phytochemical screening and antibacterial evaluation of the leaf and root extracts of Cassia alata Linn. African Journal of Pharmacy and Pharmacology 2(7), 124-129.

Farnsworth NR, 1993. Biological approaches to the screening and evaluation of natural products. In: Rasoanaivo P, Ratsimamanga-Urverg S, (eds), Biological Evaluation of Plants with Reference to the Malagasy Flora, Monograph from the IFS-NAPRECA Workshop on Bioassays, (Madagascar), 35-43.

Field M. 2003. Intestinal ion transport and the pathophysiology of diarrhea. The Journal of Clinical Investigation 111(7), 931-943.

Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S, Efferth T. 2007. Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytotherapy Research 21(10), 989-994.

Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86(6), 985-990.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. 1994. Bergey’s manual of determinative bacteriology. Edn.9, Williams & Wilkins, Co, 176.

Kaushik P, Goyel P. 2008. In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity. Indian Journal of Microbiology 48(3), 353-357.

Kim HM, Lee EH, Hong SH, Song HJ, Shin MK, Kim SH, Shin TY. 1998. Effect of Syzygium aromaticum extract on immediate hypersensitivity in rat. Journal of Ethnopharmacology 60, 125-131.

Li Y, Xu C, Zhang Q, Liu JY, Tan RX. 2005. In vitro anti-Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases. Journal of Ethnopharmacology 98(3), 329-333.

Lopez P, Sanchez C, Batlle R, Nerin C. 2005. Solid and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected food borne bacterial and fungal strains. Journal of Agricultural and Food Chemistry 53(17), 6939-6946.

Mandee Q, Hasscun A, Isa Z. 2003. Antimicrobials activity of certain spice extracts. Journal of Spices and Aromatic Crops 122, 146-153.

Miyazawa M, Hisama M. 2003. Antimutagenic activity of phenylpropanoides from clove (Syzygium aromaticum). Journal of Agricultural and Food Chemistry 51(22), 6413-6422.

Nanasombat S, Lohasupthawee P. 2005. Antimicrobial activity of crude ethanolic extracts and essential oils of spices against salmonellae and other enterobacteria, KMITL Science and Technology Journal 5(3), 527-538.

Nascimento GGF, Locatelli J, Freitas PC, Silva GL, 2000. Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria. Brazilian Journal of Microbiology 31(4), 227-156.

Okwori AEJ, Agada GOA, Olabode AO, Agina SE, Okpe ES, Okopi J. 2007. The prevalence of pathogenic Yersinia enterocolitica among diarrhea patients in Jos, Nigeria. African Journal of Biotechnology 6(8),1031-1034.

Parekh J, Chanda S. 2007. Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants against some Staphylococcus Species. Turkish Journal of Biology 32, 63-71.

Prescott LM, Hurley PJ, Klein AD. 2005. Microbiology. Edn.6, McGraw – Hill Publisher, Singapore, 1126.

Saeed S, Naim A, Tariq P. 2007. A study on prevalence of multi-drug-resistant Gramnegative bacteria. International Journal of Biology and Biotechnolgy 4(1), 71-74.

Saeed S, Tariq P, 2008. In vitro antibacterial activity of clove against gram negative bacteria. Pakistan Journal of Botany 40(5), 2157-2160.

Salman MT, Khan RA, Shukla I. 2008. Antimicrobial activity of Nigella sativa Linn. Seed oil against multi-drug resistant bacteria from clinical isolates. Natural Product Radiance 7(1), 10-14.

Singh G, Kapoor IP, Pandey SK, Singh UK, Singh RK. 2002. Studies on essential oils: part 10; antibacterial activity of volatile oils of some spices. Phytotherapy Research 16(7), 680-702.

Sivam GP, Lampe JW, Ulness B, Swanzy SR, Potter JD, 1997. Helicohacter pyloriin vitro susceptibility to garlic (Allium sativum) extract. Nutrition and Cancer 27(2), 118-121.

Smith-Palmer A, Stewart J, Fyee L. 2001. The potential application of plant essential oils as natural food preservation in soft cheese. Food Microbiology 18, 463-470.

Snyder JD, Merson MH. 1982. The magnitude of the global problem of acute diarrhoeal disease: a review of active surveillance data. Bulletin of The World Health Organ 60(4), 605-613.

Souza EL, Stamford TLM, Lima EO, Trajano VN, Filho JB. 2005. Antimicrobial effectiveness of spices: an approach for use in food conservation systems. Brazilian Archives of Biology and Technology 48(4), 549-558.

Srivastava KC, Malhotra N. 1991. Acetyl euginol, a component of oil of cloves (Syzygium aromaticum L.) inhibits aggregation and alters arachidonic acid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids 42(1), 73-81.

Tylor JLS, Rabe T, McGraw LJ, Jager AK, van Staden J. 2001. Towards the scientific validation of traditional medicinal plants. Plant Growth Regulator 34(1), 23-37.

Yang YC, Lee SH, Lee WJ, Choi DH, Ahn YJ. 2003. Ovicidal and adulticidal effects of Eugenia cryophyllata bud and leaf oil compounds on Pediculus capitis. Journal of Agricultural and Food Chemistry 51(17), 4884-4888.

Zuckerman JN, Rombo L, Fisch A. 2007. The true burden and risk of cholera: Implications for prevention and control. The Lancet Infectious Diseases 7(8), 521-530.