In-vitro effect of domesticated animal’s saliva against puss forming bacteria

Paper Details

Research Paper 01/04/2019
Views (832)
current_issue_feature_image
publication_file

In-vitro effect of domesticated animal’s saliva against puss forming bacteria

Muhammad Muzammal, Saqib Ali Rustam, Shawana Huma, Muhammad Sohaib, Safeer Ahmad, Muhammad Zeeshan Ali, Kaleem Ullah shah, Muhammad Hanif, Rizwan, Fatima, Sohail Ahmad, Saima Mashal, Hafsa Qureshi. Safeena Sadiq
Int. J. Biosci. 14(4), 392-398, April 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Puss forming bacteria increasing their resistance against number of antibiotics, this study was done to check the antibacterial activity of domesticated animal’s saliva before and after feeding against 5 different puss forming bacteria using disc diffusion method. In this study saliva was taken from three domesticated animals i.e. dog, horse and cat and examine against five puss forming bacteria i.e. Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Salmonella typhi and Escherichia coli. Among the test saliva, highest antibacterial activity was shown by dog saliva (21+1mm before feeding &23+1mm after feeding)against Escherichia coli and its activity was lowest(16+1mmbefore feeding and19+1mm after feeding) against Staphylococcus epidermidis. In case of cat, its saliva shown highest activity (20+0.6mm before feeding &22+0.8mm after feeding) against Staphylococcus aureus and its activity was lowest (16+0.6mm before feeding &16.5+0.9mm after feeding) against Streptococcus pyogenes and in case of horse highest antibacterial activity (18+0.3mm before feeding and 18.2+0.7mm after feeding) was shown by its saliva against Salmonella typhi and lowest (16+1 mm before feeding) against Staphylococcus aureus and after feeding its activity was lowest (17.6+0.5mm) against Staphylococcus epidermidis. Before and after feeding the pH of saliva was also measured. PH of dog saliva before and after feeding was 8.46 and 8.22, for cat it were 8.1 and 7.9 and for horse it were 7.2 and 6.5 respectively. Form the result it has been suggested that animal’s saliva has high antibacterial activity against number of pathogenic bacteria and can be used as antibacterial drug in near future.

Davis SJ, Valla FR. 1978. Evidence for domestication of the dog 12,000 years ago in the Natufian of Israel. Nature 276(5688), 608.

De Almeida PDV, Gregio AM, Machado MA, De Lima AA, Azevedo LR. 2008. Saliva composition and functions: a comprehensive review. The journal of contemporary Dental Practice 9(3), 72-80.

Hart BL, Korinek E, Brennan P. 1987.Postcopulatory genital grooming in male rats: Prevention of sexually transmitted infections. physiology and behavior. 41, 321-325.

Ku BK, Jeon BY, Kim JM, Jang YB, Lee H, Choi JY, Cho SN. 2018. Investigation of bovine tuberculosis outbreaks by using a trace-back system and molecular typing in Korean Hanwoo beef cattle. Journal of veterinary science 19(1), 45-50.

Larson G, Fuller DQ. 2014. The evolution of animal domestication. Annual Review of Ecology, Evolution, and Systematics 45, 115-136.

Mandel ID, Ellison SA. 1981. Naturally occurring defense mechanisms in saliva. In: Tanzer, J. M., ed. Animal models in cardiology: Proc. symp. Workshop in cardiology. Washington, DC: Information Retrieval 367-379.

Omenka CA, Osuoha JO. 2000. Antimicrobial potency of Grapefruit seed extract on five selected pathogens. Nigerian Journal of Microbiology 14(2), 39-42.

Pickering CM, Hill W, Newsome D, Leung YF. 2010. Comparing hiking, mountain biking and horse riding impacts on vegetation and soils in Australia and the United States of America. Journal of environmental management, 91(3), 551-562.

Pollock JJ, Denepitiya L, Mackay BJ, Iacono VJ. 1984. Fungi- static and fungicidal activity of the human parotid salivary histidine- rich polypeptides on Candida albicans.Infection and immunity. 44, 702-707.

Roth L, Rosenblatt JS. 1967.Changes in self-licking during pregnancy in the rat. Journal of Comparative and Physiological Psychology. 63, 397400.

Schneirla TC, Rosenblatt JS. 1963.Tobach, E. Maternal behavior of the cat. Maternal behavior of mammals. New York: John Wiley and Sons. 122-168.

Thompson R. 1940. Lysozyme and its relation to the antibacterial properties of various tissues and secretions Archives of Pathology & Laboratory Medicine Online 30, 1096-1134.

Wang, Xiaoming, Tedford, Richard H. 2008. Dogs: Their Fossil Relatives and Evolutionary History. New York: Columbia University Press, page 1.

Zeder M. 2012. The domestication of animals. Journal of Anthropological Research, 68(2), 161-190.

Related Articles

Muscle type and meat quality of local chickens according to preslaughter transport conditions and sex in Benin

Assouan Gabriel Bonou*, Finagnon Josée Bernice Houéssionon, Kocou Aimé Edenakpo, Serge Gbênagnon Ahounou, Chakirath Folakè Arikè Salifou, Issaka Abdou Karim Youssao, Int. J. Biosci. 27(6), 241-250, December 2025.

Effects of micronutrients and timing of application on the agronomic and yield characteristics of cucumber (Cucumis sativus)

Princess Anne C. Lagcao, Marissa C. Hitalia*, Int. J. Biosci. 27(6), 214-240, December 2025.

Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

Pierre G. Tovihoudji*, Kamarou-Dine Seydou, Lionel Zadji, Sissou Zakari, Valerien A. Zinsou, Int. J. Biosci. 27(6), 201-213, December 2025.

On-farm validation of black soldier fly larvae meal as a sustainable replacement for shrimp meal in rainbow trout diets in the mid hills of Nepal

Ishori Singh Mahato, Krishna Paudel*, Sunita Chand, Anshuka Bhattarai, Int. J. Biosci. 27(6), 189-200, December 2025.

Insect fauna associated with Cucumis sativus (Cucurbitales: Cucurbitaceae) in Parakou, A cotton-growing area of central Benin

Lionel Zadji*, Mohamed Yaya, Roland Bocco, Prudencia M. Tovignahoua, Abdou-Abou-Bakari Lassissi, Raphael Okounou Toko, Hugues Baimey, Leonard Afouda, Int. J. Biosci. 27(6), 175-188, December 2025.

First record of two hymenopteran species, Brachymeria excarinata Gahan (Chalcididae) and Pteromalus sp. (Pteromalidae), as hyperparasitoids of Diadegma insulare in Senegal

Babacar Labou*, Etienne Tendeng, Mamadou Diatte, El hadji Sérigne Sylla, Karamoko Diarra, Int. J. Biosci. 27(6), 167-174, December 2025.

Hepatoprotective and antinociceptive effects of terpinolene in streptozotocin-induced diabetic peripheral neuropathic rats

Ravishankar Sarumathi, Muthukumaran Preethi, Chandrasekaran Sankaranarayanan*, Int. J. Biosci. 27(6), 156-166, December 2025.