In vitro evaluation of antibacterial and antifungal activates of Iphiona aucheri leaves extracts
Paper Details
In vitro evaluation of antibacterial and antifungal activates of Iphiona aucheri leaves extracts
Abstract
The aim of the current project was to evaluate the antibacterial and antifungal activities of Iphiona aucheri leaves methanolic extract and its n-hexane, chloroform and aqueous fractions. Fine powder of Iphiona aucheri leaves were extracted in 70% methanol and then subjected to sequential fractionation through n-hexane, chloroform and water. All the fractions were assessed for their antimicrobial characteristics using agar well diffusion assay. The results revealed that the applied extracts have significant antimicrobial characteristics at 30mg/ml concentration on gram positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes) and four gram negative bacteria (Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa) and two fungal strains, Aspergillus flavus and Aspergillus niger. The Aspergillus fumigates was found resistant to the applied samples. The maximum inhibition of bacterial growth caused by methanolic extract and its chloroform and aqueous fractions was found (13.7±0.57), (15.7±1.52) and (9.87±1.46) respectively against S. aureus while n-hexane expressed maximum inhibition (5.2±0.57) against K. pneumonia growth. The growth of A. niger was highly inhibited by chloroform fraction (47.43±1.27%), followed by methanolic extract (42.15±1.46%), aqueous fraction (34.45±0.52%) and n-hexane fraction (21.22±1.15%). The chloroform fraction with MIC values (1.6±0.35 mg/ml) and (160.75 ±0.57 µg/ml) against S. aureus and A. niger respectively was found most effective. In conclusion, the result of our study indicated that the leaves of Iphiona aucheri possess considerable antimicrobial activities and thus will be of great use in developing plant derived antimicrobial and chemotherapeutic agents.
Theuretzbacher U, Mouton J. 2011. Update on antibacterial and antifungal drugs–can we master the resistance crisis. Current opinion in pharmacology 11, 429-432.
Walsh TR, Toleman MA. 2011. The emergence of pan-resistant Gram-negative pathogens merits a rapid global political response. Journal of antimicrobial chemotherapy 67, 1-3.
Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M, Eloff JN. 2013. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC complementary and alternative medicine 13, 289.
Srivastava J, Chandra H, Nautiyal AR, Kalra SJ. 2014. Antimicrobial resistance (AMR) and plant-derived antimicrobials (PDA m s) as an alternative drug line to control infections. 3 Biotech 4, 451-460.
Lowy FD. 2003. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of clinical investigation 111, 1265-1273.
Andersson DI, Hughes D. 2011. Persistence of antibiotic resistance in bacterial populations. FEMS microbiology reviews 35, 901-911.
Sofowora A, Ogunbodede E, Onayade A. 2013. The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional, Complementary and Alternative Medicines 10, 210-229.
Kokate C, Purohit A, Gokhale S. 2002. Pharmacognosy 8 th edn Nirali Prakashan. India.
Gonzalez-Guevara JL, Gonzalez-Lavaut JA, Pino-Rodríguez S, Garcia-Torres M, Carballo-Gonzalez MT, Echemendia-Arana OA, Molina-Torres J, Prieto-Gonzalez S. 2004. Phytochemical screening and in vitro antiherpetic activity of four Erythtroxylum species. acta farmacéutica bonaerense 23, 506-509.
Das K, Tiwari R, Shrivastava D. 2010. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of medicinal plants research 4, 104-111.
Suresh S, Nagarajan N. 2009. Antimicrobial activity and preliminary phytochemical analysis of Begonia malabarica Lam. Journal of Pure and Applied Microbiology 3, 801-803.
Fernebro J. 2011. Fighting bacterial infections—future treatment options. Drug Resistance Updates 14, 125-139.
Anderberg A. 1985. The genus Iphiona (Compositae‐Inuleae). Nordic journal of botany 5, 169-194.
Kakar SA, Tareen RB, Kakar MA, Jabeen H, Kakar S-u-R, Al-Kahraman Y, Shafee M. 2012. Screening of antibacterial activity of four medicinal plants of Balochistan-Pakistan. Pakistan Journal of Botany 44, 245-250.
Lanjwani AH, Ghanghro AB, Memon FM, Memon MN, Ghanghro IH, Channa MJ. 2016. Extraction of trace minerals from some important medicinal plants growing in District of Kamber/Shahdadkot, Sind, Pakistan. Rawal Medical Journal 41, 360-362.
Bagamboula C, Uyttendaele M, Debevere J. 2003. Antimicrobial effect of spices and herbs on Shigella sonnei and Shigella flexneri. Journal of food protection 66, 668-673.
Duraipandiyan V, Ignacimuthu S. 2009. Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam. Journal of ethnopharmacology 123, 494-498.
Umadevi S, Mohanta G, Chelladurai V, Manna P, Manavalan R. 2003. Antibacterial and antifungal activity of Andrographis echiodes. Journal of Natural Remedies 3, 185-188.
Owuama CI. 2017. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research 11, 977-980.
Aliyu AB, Musa AM, Abdullahi MS, Ibrahim H, Oyewale AO. 2011. Phytochemical screening and antibacterial activities of Vernonia ambigua, Vernonia blumeoides and Vernonia oocephala (Asteraceae). world 10, 11.
Souza JM, Chang MR, Brito DZ, Farias KS, Damasceno-Junior GA, Turatti IC, Lopes NP, Santos EA, Carollo CA. 2015. Antimicrobial activity of Aspilia latissima (Asteraceae). Brazilian Journal of Microbiology 46, 1103-1110.
Ebelle Etame R, Mouokeu RS, Pouaha C, Laurel C, Voukeng Kenfack I, Tchientcheu R, Assam Assam JP, Poundeu M, Stève F, Tchinda Tiabou A. 2018. Effect of Fractioning on Antibacterial Activity of Enantia chlorantha Oliver (Annonaceae) Methanol Extract and Mode of Action. Evidence-Based Complementary and Alternative Medicine 2018. http://dx.doi.org/10.1155/2018/4831.593
Ahameethunisa AR, Hopper W. 2010. Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria. BMC complementary and alternative medicine 10, 6.
Singh H, Batish DR, Kohli R. 2001. Allelopathy in agroecosystems: an overview. Journal of Crop Production 4, 1-41.
Omezzine F, Daami-Remadi M, Rinez A, Ladhari A, Haouala R. 2011. In vitro assessment of Inula spp. organic extracts for their antifungal activity against some pathogenic and antagonistic fungi. African Journal of Microbiology Research 5, 3527-3531.
Habbu P, Mahadevan K, Shastry R, Manjunatha H. 2009. Antimicrobial activity of flavanoid sulphates and other fractions of Argyreia speciosa (Burm. f) Boj. Indian Journal of Experimental Bology 47, 121-128.
Muhammad Abdur Rehman Shah, Rahmat Ali Khan, Mushtaq Ahmed, Fazal Dian, Manzoor Ullah, 2019. In vitro evaluation of antibacterial and antifungal activates of Iphiona aucheri leaves extracts. Int. J. Biosci., 15(3), 175-182.
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