In vitro larvicidal activity of varying concentrations of madre de cacao (Gliricidiasepium Jacq.) Concentrated crude ethanolic extract againts larvae of horn fly (Haematobiairritans Linn.)
Paper Details
In vitro larvicidal activity of varying concentrations of madre de cacao (Gliricidiasepium Jacq.) Concentrated crude ethanolic extract againts larvae of horn fly (Haematobiairritans Linn.)
Abstract
A study on in vitro larvicidal activity of different levels of Madre de Cacao (Gliricidiasepium Jacq. Steud) concentrated crude ethanolic extract (CCEE) against hornfly larvae (Haematobiairritans Linn.) was conducted. The air-dried leaves of Gliricidiasepium were infused in a 1:3 ratio (w/v) using ethanol as a solvent and concentrated in a rotary evaporator (60°C). A total of 120 larvae of Haematobiairritans were exposed in various concentrations: 200, 400, 800 and 1000 ppm. Based on the result after 5 hours of exposure, CCE G. sepium extract at 200 ppm showed less effect with 30% mortality compared to 400 ppm, 800 ppm and 1000 ppm with 70%, 83% and 100% mortality, respectively. Findings also revealed that CCE of G. sepium extract at 1000 ppm, 800 ppm, and commercial larvicide were comparable in causing mortality of H. irritans larvae from the first hour up to the fifth hours of exposure. However, in the fifth hour, 400 ppm was also found to be effective. This suggests that the higher the concentration of CCE G. sepium extract and the longer the time of exposure, the higher is the percentage mortality of the larvae. Thus CCE G. sepium extract can be used as an alternative for commercial larvicide.
Canadian CouncilOf Ministers of the Environment. 2012. Canadian water quality guidelines for the protection of aquatic life: Trichlorfon. In: Canadian environmental quality guidelines, 1999, Canadian Council of Ministers of the Environment, Winnipeg. Excerpt from Publication No. 1299; ISBN 1-896997-34-1. ceqg-rcqe.ccme.ca/download/en/123
Byford RL, Craig ME, Crosby BL. 1992. A review of ectoparasites and their effect on cattle production. Journal of Animal Science 70(2), 597-602. https://doi.org/10.2527/1992.702597x
Ciccio JF, Chaverri CA. 2015. Leaf and flower essential oil compositions of Gliricidiasepium (Fabaceae) from Costa Rica. American Journal of Essential Oils and Natural Products 2(3), 18-23. www.essencejournal.com/pdf/2015/vol2issue3/PartA/2-3-6-109.pdf
Cumming JM, Murray GD. 2006. Diptera associated with livestock dung horn fly Haematobiairritans (L.). North American Dipterist Society. http://www.nadsdiptera.org//FFPLhorn.htm
De Geyter E, Geelen D, Smagghe G. 2007. First results on the insecticidal action of saponins. Communication in Agricultural and Applied Biological Sciences, Ghent University 72(3), 645-648. http://hdl.handle.net/1854/LU-410903
Detablan AS. 2013. In vitro acaricidal activity of lemongrass (Cymbopogoncitratus) solvent extracts/fraction against larvae of Rhipicephalussanguineus Latrielle. (Unpublished Undergraduate Thesis). College of Veterinary Medicine, Visayas State University, Visca, Baybay City, Leyte, Philippines.
Fitzpatrick D, Kaufman P. 2011. Horn Fly (Haematobiairritans L.). Entomology and Nematology Department. USA: University of of Florida. EENY-490. entnemdept.ufl.edu/creatures/livestock/flies/horn_fly.htm
Kaufman PE, Koehler PG, Butler PG. 1995. External Parasites on Beef Cattle. Entomology and Nematology Department. USA.edis.ifas.ufl.edu/ig130
Loftin K, Corder R. 2011.Controlling Horn Flies on Cattle. University of Arkansas Division of Agriculture .USA. https://www.uaex.edu/publications/pdf/FSA-7031.pdf
Lovaas B. 2008. Internal and external parasite control, MN 5574. Beef/Cow/Calf management veterinarian, University of Minnesota, North central Research and Outreach Center, 1861, east Highway 169, Grand Rapids, p 1.
Mock DE. 1997. Managing Insect Problems on Beef Cattle. Kansas State University Agricultural Experiment Station and Cooperative Extension Service Manhattan, Kansas. USA. www.ksre.k-state.edu>historicpublications>pubs
Pare PW, Tumlinson JH. 1999 Plant volatiles as a defense against insect herbivores. Plant Physiology 121(2), 325-332. https://doi.org/10.1104/pp.121.2.325.
Sinha SN. 2013. Phytochemical profiles and antioxidant activities of the leaf extracts of GliricidiaSepium. India. International Journal of Innovations in Bio-Sciences 3(3), 87-91. www.academia.edu
Waliwitya R, Kennedy CJ, Lawenberger CA. 2009. Larvicidal and oviposition altering activity of monoterpenoids, trans- anethol and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Management Science 65, 241– 248. https://doi.org/10.1002/ps.1675
Antonio Tangayan, Hershey P. Mondejar, Zeam Voltaire E. Amper (2020), In vitro larvicidal activity of varying concentrations of madre de cacao (Gliricidiasepium Jacq.) Concentrated crude ethanolic extract againts larvae of horn fly (Haematobiairritans Linn.); IJB, V17, N2, August, P29-35
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