In vitro response of strawberry (Fragaria x ananassa Dutch.) for callus induction and shoot regeneration
Paper Details
In vitro response of strawberry (Fragaria x ananassa Dutch.) for callus induction and shoot regeneration
Abstract
Effect of growth regulators on in vitro responses of strawberry for callus induction and shoot regeneration has been investigated. MS (Murashige and Skoog) medium supplemented with 2,4-diclorophenoxyacetic acid (3.0mg/l) and 6-Benzyl adenine (0.5mg/l) were found most effective in terms of percentage to callus inductions and degree of callus development. Calli derived strawberry explants were cultured in shoot formatting media supplemented with different concentrations of plant growth regulators. The combination of BA (1.5 mg/l) and a-Napthalene acetic acid (0.75mg/l) showed highest percentage of shoot induction (55.6±0.81) and multiple shoot regeneration. Combination of BA, NAA and KIN (6-Furfuryl amino purine/kinetin) found to be most efficient in shoot induction (60.7%) and multiplications (16.7±0.48). After successful shoot regeneration, regenerated shoots were cultured for root induction in MS0. The developed plantlets through in vitro culture technique were acclimated and successfully transferred to in vivo condition for further evaluations.
Darrow GM. 1966. The strawberry: History, Breeding and Physiology. Holt, Rinehart and Winston, New York.
Finstad K, Martin RR. 1995. Transformation of strawberry for virus resistance. Acta Hort. 385.
Jain SM, Brar DS, Ahloowalia BS. 1998. Somaclonal variation and induced mutations in crop improvement. Kluwer Academic Publishers, UK.
Jelenkovic G, Chin C, Billings S, Eberhardt J. 1991. Transformation studies in cultivated strawberry, Fragaria x ananassa Duch., The strawberry into the 21st century (Eds.), Dale A. and Luby JJ Portland Or: Timber Press. p. 91- 97.
Kartha KK, Leung NL, Pahl K. 1980. Cryopreservation of strawberry meristems and mass propagation of plantlets. J. Am. Soc. Hort. Sci. 105 (4), 481- 484.
Kaushal K, Nath AK, Kaundal P, Sharma DR. 2004. Studies on somaclonal variation in strawberry (Fragaria x ananassa duch.) cultivars. Acta Hort. 662, 269- 275.
Larkin PJ, Scowcroft SC. 1981. Somaclonal variation- a novel source of variability from cell culture for plant improvement. Theor. Appl. Genet. 60, 197-214.
Liu ZR, Sanford JC. 1988. Plant regeneration by organogenesis from strawberry leaf and runner tissue. Hort. Sci. 23 (6), 1057- 1059.
Losina-Losinskaja AS. 1926. Revision critique du genre Fragaria. Bulletin du Jardin Botanique USSR. 25, 47- 88.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant 15, 473- 497.
Naruhashi N, Iwata T. 1988. Taxonomic re-evaluation of Fragaria nipponica Makino and allied species. Journal of Phytogeography and Taxonomy 36, 59- 64.
Nehra NS, Stushnoff C. 1989. Direct shoot regeneration from strawberry leaf disks. J. Am. Soc. Hort. Sci. 114 (6), 1014- 1018.
Nehra NS, Chibber RN, Kartha KK, Datla RSS, Crosby WL, Stushnoff C. 1990. Genetic transformation of strawberry by Agrobac-terium tumefaciens using a leaf disk regeneration system. Plant Cell Rep. 9, 293- 298.
Nehra NS, Stushnoff C, Kartha KK. 1990. Regeneration of plants from immature leaf-derived callus of strawberry (Fragaria x ananassa). Plant Sci. 66, 119- 126.
Nfapp. 2003. U.S. Strawberries. National Food and Agricultural Policy 2003. U.S. Fruit and Vegetable Outlook, p 24.
Passey AJ, Barrett KJ, James DJ. 2003. Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types. Plant Cell Rep. 21, 397- 401.
Patnaik J, Sahoo S, Debata BK. 1999. Somaclonal variation in cell suspension culture-derived regenerants of Cymbopogon martinii (Roxb.) wats var. motia. Plant Breed. 118, 351- 354.
Sakila S, Ahmed MB, Roy UK, Biswas MK, Karim R, Razvy MA, Hossain M, Islam R, Hoque A. 2007. Micropropagation of Strawberry (Fragaria X ananassa Duch.) A Newly Introduced Crop in Bangladesh, American-Eurasian Journal of Scientific Research 2 (2), 151- 154.
Shamima N, Hossain MM, Khatun A, Alam A, Mondal MR. 2003. Induction and evaluation of somaclonal variation in potato (Solanum tuberosum L.) Inl. J. Biol. Sci. 3(2), 183- 190.
Staudt G, Dickore WB. 2001. Notes on Asiatic Fragaria species: Fragaria pentaphylla Losinsk. and Fagaria tebetica spec. nov. Bot. Jahrb. Syst. 123, 341-355.
Staudt G. 1962. Taxonomic studies in the genus Fragaria. Typification of Fragaria species known at the time of Linnaeus. Can. J. Bot. 40, 869- 886.
Staudt G. 1989. The species of Fragaria, their taxonomy and geographical distribution. Acta Hort. Cult. 265, 23- 33.
Staudt G. 1999. Systematics and geographic distribution of the American strawberry species. Taxonomic studies in the genus Fragaria. Univ. Calif. Publ. Bot. 81.
M Rezaul Karim, M Abdul Aziz, Uthpal Krishna Roy, M Aminul Hoque, M Monzur Hossain, 2011. In vitro response of strawberry (Fragaria x ananassa Dutch.) for callus induction and shoot regeneration. Int. J. Agron. Agric. Res., 1(1), 29-36.
Copyright © 2011 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.