In vivo study of stress oxidative and some organs damage in rats exposed to metribuzin (triazinone herbicide)
Paper Details
In vivo study of stress oxidative and some organs damage in rats exposed to metribuzin (triazinone herbicide)
Abstract
The study was conducted to investigate the effects of pesticides Metribuzin (Triazinone herbicide) which is used worldwide as an ecotoxic herbicide and is the most widely used in Algeria and in random ways without respect for the doses approved, on oxidative stress and liver structure in rats. About 30 female rats Wistar albinos divided into five groups (n=6), the first group of non-treated rats serve as control, the second group of rats treated with 30 mg kg–1 b.wt., of Metribuzin and the third group of rats treated with 30 mg kg–1 b.wt. of Metribuzin followed by aqueu extrcts of Astragalus gombo (roots) with dose 80 mg/kg during 15 days, the fourth group treated with 30 mg kg–1 b.wt. of Metribuzin followed by aqueu extrcts of Astragalus gombo (leaves) with dose 60 mg/kg during 15 days and the fifth group of rats treated with 30 mg kg–1 b.wt. of Metribuzin, followed by drug (vitamin E) with dose 200 mg/ml. Metribuzin added in their drinking water for 30 days. This plant administered in combination with the pesticide shows a highly significant decrease (p≤0.01) in maintaining the equilibrium of the antioxidant/prooxidant balance cells of all tissues studied. The treatment with the two extracts of Astragalus gombo decreases the state of oxidative stress induced by metribuzin exposure by limiting free radical phenomena and repairing oxidative damage by decreasing lipid peroxidation in the liver, kidney and liver.
Adhikari N, Sinha N, Narayan R. 2001. Lead- Induced Cell Death in Testes of Young Rats. Journal of Applied Toxicology 21(4), 275-277. http://dx.doi.org/10.1002/jat.754
Ahmed Marwa A, Hassanein Khaled MA. 2013. Cardio protective effects of Nigella sativa oil on lead induced cardio toxicity: Anti-inflammatory and antioxidant mechanism. J. Physiol. Pathophysiol., 4, 72-80. http://dx.doi.org/10.5897/JPAP2013.0083
Bradford MM. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry 72, 248-254. http://dx.doi.org/10.1016/0003-2697(76)90527-3
Bunge A. 1868. Generis Astragali species Gerontogeae. Parsprior. Claves diagnosticae. Mémoire. Academie Imperiale des Sciences de St. Petersbourg, ser. 7(11), 1-140.
Chouanna T. 2017. Caractérisation structurale et activités biologiques des polysaccharides d’Astragalus gombo bunge. Thése doctorat Centre nationale de recherche scientifique, umr 6602, institut Pascal, 63178 Aubière, France
Das KS, Balakrishnan V, Mukherjee S, Vasudevan DM. 2008. Evaluation of blood oxidative stress-related parameters in alcoholic liver disease and non-alcoholic fatty liver disease. The Scandinavian Journal of Clinical & Laboratory Investigation 68, 323–334. https://dx.doi.org/10.1080/00365510701673383.
Datta HS, Mitra SK, Paramesh R, Patwardhan B. 2011. Theories and Management of Aging: Modern and Ayurveda Perspectives. Evidence-Based Complementary and Alternative Medicine. http://dx.doi.org/10.1093/ecam/nep005
Esrefoglu M. 2012 Oxidative stress and benefits of antioxidant agents in acute and chronic hepatitis. Hepat. Mon 12, 160–167. http://dx.doi.org/10.5812/hepatmon.837
Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of ageing. Nature. Frontiers in Drug Design & Discovery, 8, 239–24. http://dx.doi.org/10.1038/35041687
Groenbaek K, Friis H, Hansen M, Ring-Larsen H and Krarup HB. 2006. The effect of antioxidant supplementation on hepatitis C viral load, transaminases and oxidative status: A randomized trial among chronic hepatitis C virus-infected patients. European Journal of Gastroenterology & Hepatology. 18, 985–989. http://dx.doi.org/10.1097/01.meg.0000231746.761364a
Sarkar S, Mukherjee S, Chattopadhyay A and Bhattachary S. 2014. Low dose of arsenic trioxide triggers oxidative stress in zebrafish heart: Expression of antioxidant genes. Ecotoxicology and Environmental Safety 107, 1-8, http://dx.doi.org/10.1016/j.ecoenv.2014.05.012.
Sastre J, Pallardo FV, Asuncion J, Vina J. 2000.Mitochondria, oxidative stress and aging. Free. Radical Research 32, 189-198. http://dx.doi.org/10.1080/10715760000300201.
Selami Boukhezza. 2014. Caractérisation physicochimique et biochimique de l’Astragalus gombo (Bunge).mémoire fin d’étude ingénieure d’état technologie –alimentaire, Université de. Ouargla, 62 pages.
Shalan MG, Mostafa MS, Hassouna MM, Hassab SE, El-Nabi AE. 2005. Amelioration of lead toxicity on rat liver with vitamin C and Silymarin supplements. Toxicology 206(1), 1-15. http://dx.doi.org/10.1016/j.tox.2004.07.006
Simeonova R, Vitcheva V, Kondeva-Burdina M, Krasteva I, Manov V, Mitcheva M. 2013. Hepatoprotective and antioxidant effects of saponarin, isolated from Gypsophila trichotoma Wend. On paracetamol-induced liver damage in rats. BioMed Research International 2013, 757126. http://dx.doi.org/10.1155/2013/757126.
Videla LA. 2009. Oxidative stress signaling underlying liver disease and hepatoprotective mechanisms. World Journal of Hepatology 1, 72–78. http://dx.doi.org/10.4254/wjh.v1.i1.72
Yin L, Wei L, Fu R, Ding L, Guo Y, Tang L, Chen F. 2014. Antioxidant and hepatoprotective activity of Veronica ciliata Fisch. Extracts against carbon tetrachloride-induced liver injury in mice. Molecules 19, 7223–7236. http://dx.doi.org/10.3390/molecules19067223.
Zaheer A, Iqbal MZ, Shoro AA. 2013. Lead- Induced Reduction in Body and Kidney Weight of Wistar Albino Rats Ameliorated by Ginkgo biloba Extract (EGb 761). Biochemistry and Physiology 2, 1-4. http://dx. doi:10.4172/2168-9652.1000113.
Mahboub Nasma, Slimani Noureddine, Salhi Imane, Adaika Aicha, Khelil Aminata, 2019. In vivo study of stress oxidative and some organs damage in rats exposed to metribuzin (triazinone herbicide). Int. J. Biosci., 15(6), 397-404.
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