J. Bio. Env. Sci.5(2), 562-571, August 2014
Microcuttings browning and eventual death of the tissue during the initial stage of walnut tissue culture is a frequent problem. In this research, three different experiments were conducted to inhibition microcuttings browning. In first experiment, the effect DKW, DKW and MS culture medium fortified with activated charcoal (AC) and ascorbic acid (AA) on the initiation medium for two Persian walnut cultivars chandler and Jamal were evaluated. Results of this experiment indicated that DKW medium fortified with AC were optimum medium than MS and DKW Supplemented with AC and AA. In second experiment, the subculturing thrice of two walnut cultivars microcuttings at an interval of 48 hrs in DKW basal medium and DKW medium containing AC and AA were studied. The microcuttings subculturing in DKW medium containing AC was better than subculturing in DKW medium containing AA but there was no significant difference between them. In third experiment, the microcuttings were soaked for 2 h before culture by following solutions: soaked in distilled water, polyvinyl pyrrolidone (PVP) and AA for two walnut cultivars. Microcuttings soaked in PVP had suitable establishment than AA and distilled water. Finally, this research indicated in most cases successful control of Persian walnut microcuttings browning could be achieved by different combinations of these methods.
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