Inhibition of persian walnut (Juglans regia L.) microcuttings browning by utilizing different methods

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Research Paper 01/08/2014
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Inhibition of persian walnut (Juglans regia L.) microcuttings browning by utilizing different methods

Abdollah Ehteshamnia, Mansour Gholami
J. Bio. Env. Sci.5( 2), 562-571, August 2014.
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Abstract

Microcuttings browning and eventual death of the tissue during the initial stage of walnut tissue culture is a frequent problem. In this research, three different experiments were conducted to inhibition microcuttings browning. In first experiment, the effect DKW, DKW and MS culture medium fortified with activated charcoal (AC) and ascorbic acid (AA) on the initiation medium for two Persian walnut cultivars chandler and Jamal were evaluated. Results of this experiment indicated that DKW medium fortified with AC were optimum medium than MS and DKW Supplemented with AC and AA. In second experiment, the subculturing thrice of two walnut cultivars microcuttings at an interval of 48 hrs in DKW basal medium and DKW medium containing AC and AA were studied. The microcuttings subculturing in DKW medium containing AC was better than subculturing in DKW medium containing AA but there was no significant difference between them. In third experiment, the microcuttings were soaked for 2 h before culture by following solutions: soaked in distilled water, polyvinyl pyrrolidone (PVP) and AA for two walnut cultivars. Microcuttings soaked in PVP had suitable establishment than AA and distilled water. Finally, this research indicated in most cases successful control of Persian walnut microcuttings browning could be achieved by different combinations of these methods.

VIEWS 4

Amiri ME. 2004. Effect of mineral concentration on tissue cultured walnut (Juglans nigra var. zeiabadi) growth. International Society for Horticultural Science, p 705.

Anonymous. 1988. SAS/STAT User’s Guide, Release 6.03. Statistical Analysis System (SAS) Institute, Inc, Cary, NC.

Ashutosh A, Murkute Sh, Mayakumari P. 2003. Exudation and browning in tissue culture of pomegranate. Agricultural Science Digest 23, 29–31.

Bhat SR, Chandel KPJ. 1991. PI eellReports, 10, 358-361.

Bin Zh, Xinfang W, Rongting W, Jingming J. 2010. Quantification of the enzymatic browning and secondary metabolites in the callus culture system of Nigella glandulifera Freynet Sint. Asian Journal of traditional medicines, 5,109-116.

Block R, Lankes C. 1996. Massnahmen gegen die Verbräunung bei der in-vitro-Etablierung der Apfelunterlage M9. Gartenbauwiss 61, 11–7.

Bonga JM, Aderkas PV. 1992. In vitro culture of trees, 2, 35-38. http://dx.doi.org/10.1007/978-94-015-8058-8

Bosela MJ, Michler CH. 2008b. Medium effects on black walnut (Juglans regia L.) shoot culture growth in vitro: evaluation of multiple nutrient formulation and cytokinin types. In Vitro Cellular & Developmental Biology Plant 44, 116-121. http://dx.doi.org/10.1007/s11627-008-9114-5

Bourrain L. 2000. In vitro walnut micropropagation (Juglans nigra L.) application. CTIFL. p 30-127.

Cheng TY. 1978. Propagating woody plants through tissue culture. Am Nurseryman, 7-14.

Christopoulos MV, Tsantili E. 2011. Effects  of temperature and packaging atmosphere on total antioxidants and colour of walnut (Juglans regia L.) kernels during storage. Journal of Scientia Horticulturae 131, 49–57. http://dx.doi.org/10.1016/j.scienta.2011.09.026

Dibax R, Eisfeld CL, Koehler HC, Quoirin M. 2005. Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis. Scientia Agricola (Piracicaba, Brazil) 62, 406-412. http://dx.doi.org/10.1590/S0103-90162005000400016

Dobránszki J, Abdul-Kader A, Magyar-Tábori K, Jámbor-Benczúr E, Bubán T, Szalai J. 2000a. In vitro shoot multiplication of apple: comparative response of three rootstocks to cytokinins and auxin. Int Journal of Hortic Sci 6, 36–9.

Dobránszki J, Abdul-Kader A, Magyar-Tábori K, Jámbor-Benczúr E, Bubán T, Szalai J. Szalai. 2000b. Single and dual effects of different cytokinins on shoot multiplication of different apple scions. Int Journal of Hortic Sci 6, 76–8.

Dobránszki J, Magyar-Tábori K, Jámbor-Benczúr E, Lazányi J, Bubán T, Szalai J. 2000c. Influence of aromatic cytokinins on shoot multiplication and their post-effects on rooting of apple cv. Húsvéti rozmaring. Int Journal of Hortic Sci 6, 84–7.

Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of paradox walnut rootstock, Journal of Hort Sci 19: 507-709.

Elmore H, Samples B, Sharma S, Harrison M. 1990. Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture medium. Plant Cell, Tissue and Organ Culture, 20, 131-135. http://dx.doi.org/10.1007/BF00114711

FAOSTAT. 2011. FAOSTAT Database Results 2009. http://faostat.fao.org/site/567/default.aspxancor (accessed January 2011).

Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res 50, 151-158. http://dx.doi.org/10.1016/0014-4827(68)90403-5

Gupta PK, Nadgir AL, Mascarenhas AF, Jagannathan V. 1980. Tissue culture of forest trees: clonal multiplication of Tectona grandis L. by tissue culture. Journal of Plant Sci Lett 17, 259-268. http://dx.doi.org/10.1016/0304-4211(80)90156-X

Jaiswal VS, Amin MN. 1987. In vitro propagation of guava from shoot cultures of mature trees. Journal of Plant Physiol 130, 7-12. http://dx.doi.org/10.1016/S0176-1617(87)80296-1

Lloyd G, McCown B. 1981. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot tip culture. Proc Plant Prop Soc 30, 421-427.

M’Kada J, Dorin N, Bigot C. 1991. In vitro propagation of Arctostaphylos uva-ursi L. Sprengel.: comparison between two methodologies. Plant Cell, Tissue and Organ Culture, 24, 217–22. http://dx.doi.org/10.1007/BF00033480

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nguyen TV, Thu TT, Claeys M, Angenon G. 2007. Agrobacterium mediumted transformation of sorghum (Sorghum bicolour (L.) Moench) using an improved in vitro regeneration system. Plant Cell, Tissue and Organ Culture, 91, 155–164. http://dx.doi.org/10.1007/s11240-007-9228-1

Pan MJ, van Staden J. 1998. The use of charcoal in in vitro culture – a review. Journal of Plant Growth Regulation 26, 155–163. http://dx.doi.org/10.1023/A:1006119015972

Prajapati HA, Patel DH, Mehta SR, Subramanian RB. 2003. Direct in vitro regeneration of Curculigo orchioides Gaertn., an endangered anticarcinogenic herb. Journal of Curr Sci 84, 747-749.

Saadat YA, Hennerty MJ. 2002. Factors affecting shoot multiplication of Persian walnut (Juglans regia L.). Journal of Scientia Horticulturae 95, 257-260. http://dx.doi.org/10.1016/S0304-4238(02)00003-1

Sanchez-Zamora MA, Cos-terrer J, Frutos-Tomas D, Garcia-Lopez R. 2006. Embryo germination and proliferation in vitro of Juglans regia L. Scientia Horticulturae 108, 317- 321. http://dx.doi.org/10.1016/j.scienta.2006.01.041

Seneviratne P, Wijesekara GAS. 1996. The problem of phenolic exudates in vitro cultures of mature Hevea brasiliensis. Journal of Plantation Crops 24, 54-62.

Sharada M, Ahuja A, Kaul MK. 2003. Regeneration of plantlets via callus cultures in Celastrus paniculatus Willd–A rare endangered, medicinal plant. Journal of Plant Biochem Biotechnol 12, 65-69. http://dx.doi.org/10.1007/BF03263163

Sharma M, Modgil M, Sharma DR. 2000. Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol. Indian Journal of Exp Biol 38, 1236–40.

Strosse H, Van den Houwe I, Panis B. 2004. Banana cell and tissue culture – review. In: Jain, S.M., Swennen R (eds.) Banana Improvement: Cellular, Molecular Biology, and Induced Mutations, Science Publishers, Inc., Enfield, NH, USA. p. 1-12.

Tessitura T, Tsukuda K, Kawase K. 2002. Micropropagation of shinano walnut (Juglans regia L.). Journal Japan Soc Hort Sci 17, 661-666.

Thomas TD. 2008. The role of activated charcoal in plant tissue culture. Journal of Biotechnol Adv 26, 618–31. http://dx.doi.org/10.1016/j.biotechadv.2008.08.003

Tisserat B. 1979. Propagation of data palm (Phoenix dactylifera L.) in vitro. Journal of Experimental Botany 30, 1275–1283.

Toosi S, Dilmagani K. 2010. Proliferation of Juglans regia L. by in vitro embryo Culture. Journal of Cell Biology and Genetics, 1, 12–19. http://dx.doi.org/10.5897/2141-6516

Tyagi AK, Rashid A, Maheshiwari SC. 1981. Promotive effect of polyvinyl polypyrrolidone on pollen embryogenesis in Datura innoxia. Journal of Physiology Plants 53, 405-406. http://dx.doi.org/10.1111/j.1399-3054.1981.tb02722.x

Weatherhead M, Burdon J, Henshaw G. 1979. Effects of activated charcoal as an additive to plant tissue culture medium: Part 2. Z Pflanzen. Physiol 94, 399-405. http://dx.doi.org/10.1016/S0044-328X(79)80223-8

Zhong D, Michaux-Ferriere N, Coumans M. 1995. Assay for doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? Part 1. In vitro anther culture. Plant Cell, Tissue and Organ Culture, 41, 91-97. http://dx.doi.org/10.1007/BF00051577