Inhibition of some phytopathogenic fungi by spear grass Heteropogon contortus (L.) Buauv extract
Paper Details
Inhibition of some phytopathogenic fungi by spear grass Heteropogon contortus (L.) Buauv extract
Abstract
The use of chemical herbicides for the control of phytopathogenic fungi is environmentally undesirable, hence the search for a more eco-friendly approach. This research is intended to determine the inhibitory potential of Heteropogon contortus (L.) Buauv, on some phytopathogenic fungi. Soil samples from fifty (50) farmlands in Ethiope east Local Government Area of Delta State, were aseptically collected and assessed for the presence of phytopathogenic fungi. By serial dilution, spread plate technique, cultural characteristics as well as microscopic examination, Fusarium sp., Alternaria sp. and Aspergillus sp. were isolated in percentage occurrences of 34%, 42%, and 64% respectively. Fresh leaves of Heteropogon contortus (L.) buauv were collected, rinsed in running water and air dried and then grounded to fine powder, which was extracted using methanol and water. Phytochemicals recovered included; Flavonoids, Alkaloids, Tannins, Phenols, Terpenoids, and Saponins. Poisoned plate technique was used for percentage inhibition of fungal mycelia growth using the aqueous extract, after three and five days. Alternaria sp. had a 100% inhibition, at days three and five, using a concentration of 7µL/mL and 5µL/ respectively. Aspergillus sp. exhibited a 100% mycelia inhibition at days three and five, using concentrations of 5µL/mL and 3µL/mL respectively. Fusarium sp. on the other hand, had a 100% mycelia inhibition at days three and five, using concentrations of 8µL/mL and 6µL/mL respectively. This study reveals inhibition of the phytopathogenic fungi and this inhibition increases with increasing concentrations and period of exposure. Spear grass may prove useful after all as an inhibitor of phytopathogenic fungi.
Agrios GN. 2004. Losses caused by plant diseases. Plant Pathology. (pp. 29-45) Elsevier, Oxford, UK.
Aguiar RWDS, Ootani MA, Ascencio SD, Ferreira TPS, Santos GRD, Santos MMD. 2014. Fumigant antifungal activity of Corymbia citrodora and Cymbopogon nardus essential oils and citronellal against three fungal species. The Scientific World Journal 9, 112-119.
Ahlem N, Hayfa J, Rabiaa H, Rania ABA, Sined M, Mejda D. 2016. Antifungal activity of aqueous and organic extracts from Withania somnifera L. against Fusarium oxysporium f. sp. radicis-lycopersici. Journal of Microbial and Biotechnology 8(3), 144-150. DOI: 10.4172/ 1948-5948.1000277
Ainit S. 2015.The Origin of Cultivation and Proto-Weeds, Long Before Neolithic Farming. Agricultural Science 10, 13-22.
Angeline WM, John MW, Gakuubi MM. 2017. Antifungal activity of essential oil of Eucalyptus camaldulensis Dehnh. against selected Fusarium spp. International Journal of Microbiology. Hindawi Publishing Corporation.
Athul S, Arul S,Manonmani HK. 2017. The Antimicrobial Properties of Triticum aestivum (Wheat Grass) Extract. International Journal of Biotechnology for Wellness Industries 4, 84-91.
Bhavya T, Padma S. 2014. Antifungal activity of Cymbopogon citratus leaves extract against herbal drug contaminants. World Journal of Pharmacy and Pharmaceutical Sciences 3(6), 768-775.
Bibhuti BK, Lakhi RS, Munmi B. 2013. Antimicrobial activity and phytochemical screening of some common weeds of Asteraceae family. International journal of Pharmaceutical Science Review 23(1), 116-120.
Chinen-chun L, Hsiang-Fu Y, Chun-hua C, Yun-ta L, Hsien-tsung Y. 2018. Effects of lemon grass oil and citral on hepatic drug metabolizin enzymes, oxidative stress and acetaminophen toxicity in rats. Journal of Food and Drug Analysis 26(1), 432-438.
Clara C, Matasyoh JC, Wagara IN, Nakuvuma J. 2013. Antifungal activity of Monathotaxis littoralis essential oil against mycotoxigenic fungi isolated from maize. International Journal of Microbiology Research and Reviews 2(6), 103-109.
Connell L, Redman RS, Craig S, Rodriguez RJ. 2006. Distribution and abundance of fungi in the soils of Taylor Valley, Antarctica. Soil Biology and Biochemistry 38, 3083-3094.
Dhale DA. 2013. Surface Mycoflora of Stored Part of Herbal Medicine. International Journal of Pharmacology and Biological Science 4(3), 568-574.
Dhanalakshmi V, Jeyanthi RL, Santosh K, Sashi B, Sharmila S, Susithra G. 2012. Isolation, identification and characterization of fungi from rhizophere soil of Baleriacristata. International Journal of Horticultural and Crop Science Research 2(1), 2249-4243.
Dwivedi SK, Singh KP. 1998. Fungitoxicity of some higher plant products against Macrophomina phaseolina (Tassi) Goid. Flavour Fragrance Journal 13, 397-399.
Jat JG, Agalave HR. 2013. Fungitoxic properties of some leaf extracts against oilseed-borne fungi. Science Research Reporter 3, 210-215.
Javaid A, Akhtar R. 2015. Antifungal activity of methanolic root extract of Withania somnifera against Fusarium oxysporum f. sp. cepae. African journal of Traditional Complement Alternative Medicine 12, 22-27.
Karnwal P,Singh P. 2006. Antifungal activity of Cassia fistula leaf extract against Candida albicans. Iran Journal of Microbiology 46(2), 169-170.
Katooli N, Maghsodlo R, Razavi SE. 2011. Evaluation of eucalyptus essential oil against some plant pathogenic fungi. Journal of Plant Breeding and Crop Science 3(2), 41-43.
Kim DI, Park JD, Kim SG, Kuk H, Jang MJ, Kim SS. 2005. Screening of some crude plant extracts for their acaricidal and insecticidal efficacies. Journal of Asian-Pacific Entomology 8, 93-100. DOI:10.1016/S1226-8615(08)60076-X
Muhammad A, Munir O, Muhammad S, Aqeel A, Ahmet A. 2012. Crop production for Agricultural Improvement. Springer. 525.
Nagamani J, Suresh J, Ahuja V, Reddy E. 2012. Comparative phytochemical screening of Vatashunga, Shatavari and Shatapushpa claimed for Prajasthapana activity. Annal of Biology Resources 3, 1294-1304.
Phatangare AT, Shinde HS, Mane VA. 2015. Antimicrobial investigation of Partheniun hysterophorus Linn. leaf extract against plant pathoigenic and beneficial microorganisms. International Journal of Agriculture, Innovation and Research 3(5), 1537-1539.
Philippe S, Souaibou F, Guy A. 2012. Chemical composition and antifungal activity of essential oil of fresh leaves of Ocimum gratissimum from Benin against six mycotoxigenic fungi isolated from traditional cheese wagashi. Research Journal of Biological Sciences 1, 22-27.
Siramom P, Ohtani Y, Ichiura H. 2013. Chemical composition and antifungal property of Eucalyptus camaldulensis leaf oils from Thailand. Records of Natural Products 7, 49-53.
Strange RN, Scott PR. 2005. Plant diseases: a threat to global food security. Annual Review of Phytopathology 43, 83-116.
Vishnu SG, Prasanna R, Gayathri VP. 2016. Antifungal Activity of Grass Extract. International Journal of Pharmacy, Science and Revolution Resources 39(1), 237-239.
E. M. Ilondu, D. A. Ehwarieme, E. Ebohon (2020), Inhibition of some phytopathogenic fungi by spear grass Heteropogon contortus (L.) Buauv extract; IJMM, V11, N3, March, P1-6
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