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Intestinal colonization with multidrug-resistant enterobacteriaceae in a healthy adult population

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Research Paper 01/07/2021
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Intestinal colonization with multidrug-resistant enterobacteriaceae in a healthy adult population

WV. Vandana K. Dinesha, MK. Kalaivanib, Sofiya Rabye
Int. J. Micro. Myco.13( 1), 18-25, July 2021.
Certificate: IJMM 2021 [Generate Certificate]
Key: AmpC β-lactamasesColonizationESBLGut microbiomeMultidrug-resistant organisms

Abstract

Intestinal colonization of healthy individuals with multidrug-resistant bacteria could contribute to the amplification of resistant bacteria both at community level and in hospital settings leading to treatment failure, high treatment costs, and increased mortality. This cross-sectional study aims is to determine the rate of intestinal colonization with multidrug resistant bacteria belonging to Enterobacteriaceae family among adult healthy population in a suburban community of Bangalore. 163 stool specimens from 163 participants were obtained from four suburban areas of East Bangalore during a period of 4 months (Oct, 2020–Jan, 2021). A total 165 isolates were identified. Stool specimens were cultured and the isolates were subjected to antimicrobial susceptibility tests according to the standard microbiological procedures. ESBLs, AmpC β-lactamase, and carbapenemase producers were screened and identified phenotypically as per CLSI guidelines. Colonization of MDR enteric bacteria in the community was 70%. Among these MDROs, Klebsiella was the predominant bacteria 53(45.6%) followed by E. coli 45(38.8%) and Enterobacter 17(14.6%). Confirmed ESBL producers were 47(28.4%) which included E. coli 24 (51%), Klebsiella 18 (38.2%) and Enterobacter 5 (10.6%). Confirmed AmpC producers were 8 (5%) among which Klebsiella were 5 (62.5%) and E. coli 3 (37.5%). 3 (1.8%) isolates were coproducers of ESBL and AmpC β lactamases. No carbapenemase-producing bacteria were isolated. This study shows an overall prevalence of MDRO in the intestines of study population and highlights the importance of routine surveillance to monitor the changing epidemiology. It also helps to identify the causal associations for appropriate infection control practices and antibiotic prescribing policies to prevent further spread.

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