Intra-specific variations of Rubus sp. (Rosaceae) in Northern Iran: morphometric analysis and microsatellite markers
Paper Details
Intra-specific variations of Rubus sp. (Rosaceae) in Northern Iran: morphometric analysis and microsatellite markers
Abstract
During the growing season, young raspberry leaf samples were collected from natural populations in northern Iran. A total of 65 Rubus samples were collected at altitudes ranging from 0 to 1021 meter above sea level. Morphometric and molecular studies were performed in seven Rubus species and four uncertain species from the sect. Rubus L. 7 species including R. caecius, R. sanctus, R, discolor, R. dolichocarpus, R. hyrcanus, R. persicus and R. hirtus are. In total, the delimitation of species of Rubus northern Iran, from 27 morphological characters and 13 SSR primer pairs were used. The dendrogram based on quantitative morphological traits, species are completely separated, and even species were differentiated on the basis of geographical pattern. A total of 12 loci and 29 alleles were detected. The number of alleles observed for each locus ranged from 1 to 6, with an average of 3.08 alleles per locus. The SSRs achieved cumulative polymorphism information content (PIC) of 0.9444. These PIC values demonstrated that, in spite of the low number of primers used, the SSR were sufficiently polymorphic and informative. Expected heterozygosities (He) were 0.021 to 0.286. The dendrogram based on microsatellite markers, no clear geographic pattern to interpret the results, we do not see. Status is unknown species and genotypes with high affinity and proximity to each other show. In general, the limits of species, the occurrence of hybridization between species with different ploidy levels between Rubus genotypes, is not subject to any specific geographic pattern.
Alice LA. 2002. Evolutionary relationships in Rubus (Rosaseae) based on molecular data. Acta Horticulturae 585, 79 – 83.
Alice LA, Eriksson T, Eriksen B, Campbell CS. 1997. Intersubgeneric hybridization between a diploid raspberry, Rubus idaeus, and a tetraploid blackberry, R. caesius (Rosaceae). American Journal of Botany 84, 171.
Amsellem L, Dutech C, Billotte N. 2001. Isolation and characterization of polymorphic microsatellite loci in Rubus alceifolius Poir. (Rosaceae), an invasive weed in La Reunion Island. Molecular Ecology Notes 1, 33–35.
Anderson JA, Churchill GA, Autrique JE, TanksleySD, Sorrells ME. 1993. Optimizing parental selection for genetic linkage maps. Genome 36, 181-186.
Antonius-Klemola K. 1999. Molecular markers in Rubus (Rosaceae) research and breeding. The Journal of Horticultural Science & Biotechnology 74(2), 149– 160.
Ballington JR, Luteyn MM, Thompson K, Romoleroux K, Castillo R. 1993. Rubus and Vacciniaceous germplasm resources in the Andes of Ecuador. Plant Genetic Resources Newsletter 93, 9– 15.
Billotte N, Lagoda PJL, Risterucci AM, Baurens FC. 1999. Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54(4), 277–288.
Campbell CS, Alice LA. 1999. Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany 86, 81-97.
Castillo NR. 2006. Fingerprinting and genetic stability of Rubus using molecular markers. M.Sc. Thesis. Oregon State University, USA.
Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13–15.
Excoffier L, Lischer HEL. 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10, 564 567.
Flores NR, Reed BM, Graham J, Fernandez-Fernandez F, Bassil NV. 2010. Microsatellite markers for raspberries and blackberries. Journal of the American Society for Horticultural Science 135 (3), 271–278.
Focke WO. 1914. Species Ruborum. Bibliotheca Botanica 72, 1-274.
Graham J, Mcnicol RJ. 1995. An examination of the ability of RAPD markers to determine the relationships within and between Rubus species. Theoretical and Applied Genetics 90, 1128-1132.
Kollmann J, Steinger T, Roy BA. 2000. Evidence of sexuality in European Rubus (Rosaceae) species based on AFLP and allozyme analysis. American Journal of Botany 87(11), 1592–1598.
Leonor MM, López A, Aguilar S. 2007. Genetic diversity of wild and cultivated Rubus species in Colombia using AFLP and SSR markers. Crop Breeding and Applied Biotechnology 7, 242-252.
Ataei-E Jaliseh Somayeh, Mehregan Iraj, Tarang Alireza, Nejhadsattary Taher (2014), Intra-specific variations of Rubus sp. (Rosaceae) in Northern Iran: morphometric analysis and microsatellite markers; JBES, V5, N1, July, P189-198
https://innspub.net/intra-specific-variations-of-rubus-sp-rosaceae-in-northern-iran-morphometric-analysis-and-microsatellite-markers/
Copyright © 2014
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0