Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines
Paper Details
Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines
Abstract
The traditional use of herbal medicine has increased significantly in these recent years. Nowadays it is very desirable to investigate and discover new antibacterial and anticancer agents from natural products and medicinal plants. The natural products obtained from organisms such as medicinal plants (secondary metabolites) are known as a powerful source to supplement therapy and prevention of cancer. The aim of this study was to evaluate the cytotoxic effects of Hypericum perforatum L. Extracts on cancerous cells. Sample were collected And After drying at room temperature, samples extracted by water and ethanol. Mouse breast (4T1) cancer cell lines incubated with different concentrations of ethanol extract for 48 hours and cell growth inhibition was determined using MTT assay. In addition the antioxidant activity of these extract were carried out by using several methods. Results of MTT assay showed strong and dose-dependent inhibition of cancer cell growth by ethanolic extract of Hypericum perforatum L. This extract caused a significant decrease in proliferation of tested cancer cell lines. the experimental finding show that the ethanolic extract of Hypericum perforatum L. possess significant anticancer and antioxidant activity ,justifying the use of these plants in traditional medicine ,which may be developed as phytomedicines.
Ajith TA, Janardhanan KK. 2007. Indian medicinl mushrooms as a source of antioxidant and antitumor agents. Journal of Clinical Biochemistry and Nutrition 40, 157–162. http://dx.doi.org/10.3164/jcbn.40.157
Butterweck V, Schmidt M St. 2007. Role of active compounds for its mechanism of action and efficacy. Wien Med Wochenschr 157, 356-61. http://dx.doi.org/10.1007/s10354-007-0440-8
Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anals Biochem 239, 70–76. http://dx.doi.org/10.1006/abio.1996.0292
Chopra I, Hodgson J, Metcalf B, Poste G. 1997. The search for antibacterial agents effective against bacteria resistant to multiple antibiotics. Antimicrob Agents Chemother 4, 497–503.
Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences 74, 2157–2184. http://dx.doi.org/10.1016/j.lfs.2003.09.047
Chen ZY, Chan PT. 1996. Antioxidative activity of green tea catechins in canola oil. Chemistry and Physics of Lipids 82, 163–172. http://dx.doi.org/10.1016/0009-3084(96)02587-X
Cheeseman KH, Slater TF. 1993. An introduction to free radical biochemistry. British Medical Bulletin 49, 481–493.
Carlo IGT, Paola MSP. 2009. Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All. Journal of Pharmaceutical and Biomedical Analysis 50, 440–448. http://dx.doi.org/10.1016/j.jpba.2009.05.032
Eshan B, Amitabye L-R, Alan C, Okezie I. 2004. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. Journal of the Science of Food and Agriculture 84(12), 1553–1561. http://dx.doi.org/10.1002/jsfa.1820
Elizabeth A, Mazzio F, Soliman A. 2009. In Vitro Screening for the Tumoricidal Properties of International Medicinal Herbs. Phytotherapy Research 23(3), 385–398. http://dx.doi.org/10.1002/ptr.2636
Guedes AP, Franklin G, Fernandes-Ferreira M. 2012. Essential oil composition and biologic activities. Phytochemistry Reviews 11, 127–152. http://dx.doi.org/10.1007/s11101-012-9223-y
Hua-Bin L, Chi-Chun W, Ka-Wing C, Feng C. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. Learning with Technologies 41, 385–390. http://dx.doi.org/10.1016/j.lwt.2007.03.011
Kanakis CD, Tarantilis PA, Polissiou MG, Diamantoglou S, Tajmir-Riahi HA. 2006. Antioxidant flavonoids bind human serum albumin. Journal of Molecular Structure 798, 69–74. http://dx.doi.org/10.1016/j.molstruc.2006.03.051
Leandro JL, Fadil BM, Bego-a RL, José Ignacio RS. 2010. Antioxidant activity and polyphenol content of aqueous extracts from Colombian Amazonian plants with medicinal use. Food Chemistry 119, 1566–1570. http://dx.doi.org/10.1016/j.foodchem.2009.09.043
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55–63. http://dx.doi.org/10.1016/0022-1759(83)90303-4
Fukushim S, Hasegawa A, Shibata M, Ogiso T. 1983. Carcinogenicity of butylated hydroxy anisole in F344 rats. Journal of the National Cancer Institute 70, 343-347.
Ordon Ez AAL, Gomez JD, Vattuone MA, Isla MI. 2006. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chemistry 97, 452–458. http://dx.doi.org/10.1016/j.foodchem.2005.05.024
Patricia D, Sang Chul J, Samiuela L, Cheang K, Sundar Rao K. 2012. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese Medicine 7, 26. http://dx.doi.org/10.1186/1749-8546-7-26
Rios JL, Recio MC. 2005. Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology. 100, 80–84. http://dx.doi.org/10.1016/j.jep.2005.04.025
Sanjeev S, Sanjay G.Apigenin. 2010. A Promising Molecule for Cancer Prevention. Pharmaceutical Research. 27(6), 962–978. http://dx.doi.org/10.1007/s11095-010-0089-7
Souâd A, Dalila B, Dalila S, Korrichi L. 2010. Antibacterial, antioxidant and acute toxicity tests on flavonoids extracted from some medicinal plants. International journal of green pharmacy 4(3), 165-169.
Tanja M, Slavica S, Slobodan S. 2007. In Vitro Study of Ethanolic Extract of Hypericum perforatum L. on Growth and Sporulation of Some Bacteria and Fungi. Turkish Journal of Biology 31, 237-241.
Wach A, Pyrzyńska K, Biesaga M. 2007. Quercetin content in some food and herbal samples. Food Chemistry. 100, 699-704. http://dx.doi.org/10.1016/j.foodchem.2005.10.028
Wolfe K, Wu X, Liu RH. 2003. Antioxidant activity of apple peels. Journal of Agriculture and Food Chemistry. 51, 609–614. http://dx.doi.org/10.1021/jf020782a
Yen GH, Chen HY. 1996. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry 43, 27–32. http://dx.doi.org/10.1021/jf00049a007
Yoshino M, Murakami K. 1998. Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anals Biochem. 257, 40-44. http://dx.doi.org/10.1006/abio.1997.2522
Anoosh Eghdami, Hossein Piri, Majid Sirati-Sabet, Dariush Ilghari (2013), Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines; IJB, V3, N12, December, P265-272
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