Investigation of the effect of three medicinal plants on chicken Eimeria tenella coccidiosis

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Research Paper 01/10/2019
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Investigation of the effect of three medicinal plants on chicken Eimeria tenella coccidiosis

Hervé Brice Dakpogan, Razaki Adiho Ossè, Sabbas Attindehou, Luc Hippolyte Dossa, Frédéric Houndonougbo, Armand Bienvenu Gbangboche, Guy Apollinaire Mensah, Saïdou Salifou
Int. J. Biosci.15( 4), 15-22, October 2019.
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Resistance of some coccidia strains to conventional anticoccidial products and the presence of anticoccidial drug residues in poultry products, detrimental to consumer health have led to the search for alternative means of controlling coccidiosis. The aim of the current study was to evaluate the effect of leaf extract of Morinda lucida, Combretum micranthum and Paullinia pinnata on Eimeria tenella experimental coccidiosis in a completely randomized design. There were five experimental groups: Morinda lucida, Combretum micranthum, Paullinia pinnata, amprolium treated chicks group and finally the infected untreated control chicks group. Body weight gain, feed conversion ratio, lesion score, proportion of bloody droppings, survivability, morbidity and oocyst excretion were evaluated. The results demonstrated the efficacy of Morinda lucida on Eimeria tenella coccidiosis comparable to that of amprolium, the conventional anticoccidial product, in terms of improved feed conversion ratio, total absence of bloody droppings, and reduction of oocyst excretion with a reduction rate of 81% compared to the untreated infected control group. Morinda lucida is followed in its effectiveness by Combretum micranthum in a lesser extent. Morinda lucida has proven effective in chicken coccidiosis controlling. However, investigations are needed to determine the appropriate dose, the appropriate extraction method, the parts of the plant with better anticoccidial activity, and the adequate mode of utilization, curative or preventive.


Abbas RZ, Iqbal Z, Sindhu ZD, Khan MN, Arshad M. 2012. Identification of cross resistance and multiple in Eimeria tenella field isolates to commonly used anticoccidials in Pakistan. Poultry Research 17, 361 – 368.

Ademola SG, Shittu MD, Ayansola MO, Lawal TE, Tona GO. 2013. Effect of Maxigrain supplement on growth performance, economic indices and haematological parameters of heat-stress broilers fed three dietary fiber sources. Online Journal of Animal Feed Research 3(4), 159-164.

Adesogan EK. 1973. Anthraquinones and anthraquinols from Morinda lucida. The biogenetic significance of oruwal and oruwalol. Tetrahedron letters 29, 4099 – 102.

Al-Gawad AA, Mahdy OA, El-Massry AAN, Al-Aziz MSA. 2012. Studies on coccidian of Egyptian balady breed chickens. Life Science Journal 9(3), 568-576.

Allen PC, Danforth HD, Augustine PC. 1998. Dietary modulation of avian coccidiosis. International Journal of Parasitology 28, 1131– 40.

Arczewska-Wlosek A, Swiatkiewicz S. 2010. Response of chickens infected with coccidiosis to herbal extracts mix fed singly or in combination with additives. XIIIth European Poultry Conference, p 45-50.

Banfi S, Caruso E, Orlandi V, Barbieri P, Cavallari S, Vigano P, Clerici P, Chiodaroli L. 2014. Antibacterial activity of leaf extracts from Combretum micranthum and Guiera senegalensis (Combretaceae). Research Journal of Microbiology 9, 66-81.

Bennett R, Ijpelaar J. 2005. Updated estimates of the costs associated with thirty-four endemic livestock diseases in Great Britain: a note. Journal of Agricultural Economics 56, 135–144.

Cannavan A, Ball G, Kennedy DG. 2000. Nicarbazine contamination in feeds as a cause of residue in eggs. Food Additive Contamination 25, 829-836.

Chapman H, Jeffers T, Williams R. 2010. Forty years of monensin for the control of coccidiosis in poultry. Poultry Sciences 89, 1788–1801.

Dakpogan HB, Mensah S, Attindehou S, Chystomone C, Aboh A, Naciri M, Salifou S, Mensah GA. 2018. Anticoccidial activity of Carica papaya and Vernonia amygdalina extract. International Journal of biological and chemical sciences 12(5), 2101-2010.

Danaher M, Campbell K, O’Keeffe M, Capurro E, Kennedy G, Elliott CT. 2008. Survey of the anticoccidial feed additive nicarbazin (as dinitrocarbanilide residues) in poultry. Food Additive Contamination 25, 32-40.

Drägan LA, Györke JF, Ferreira IA, Pop I, Dunca M. 2014. Effects of Artemisia annua and Foeniculum vulgare on chickens highly infected with Eimeria tenella (phylum Apicomplexa). Acta Veterinaria Scandinavica 56, 22–28.

Elmusharaf MV, Bautista L, Nollet A, Beynen. 2006. Effect of an oligosaccharide preparation on Eimeria tenella infection in broiler chickens. International Journal of Poultry Sciences 5, 583–588.

Eloff JN, Katerere R, McGaw LJ. 2008. The biological activity and chemistry of the southern African Combretaceae. Journal of Ethnopharmacology 119(3), 686-699.

Emilio DC, Margarita G, María F, Joaquín Q, Caridad SA. 2010. Effect of artemisinin on oocyst wall formation and sporulation during Eimeria tenella infection. Parasitology International 59(4), 506-511.

Gotep JG, Tanko JT, Forcados GE, Muraina IA, Ozele N, Dogonyaro BB, Oladipo OO, Makoshi MS, Akanbi OB, Kinjir H, Samuel AL, Onyiche TE, Ochigbo GO, Aladelokun OB, Ozoani HA, Viyoff VZ, Dapuliga CC, Atiku AA, Okewole PA, Shamaki D, Ahmed MS, Nduaka CI. 2016. Therapeutic and Safety Evaluation of Combined Aqueous Extracts of Azadirachta indica and Khaya senegalensis in Chickens Experimentally Infected with Eimeria Oocysts. Journal of Parasitology Research 3, 3-4.

Jimoh FO, Sofidiya MO, Afolayan AJ. 2007. Antioxidant Properties of the Methanol Extracts from the Leaves of Paullinia pinnata. Journal of Medicinal Food 10(4), 707 – 11.

Johnson J, Reid WM. 1970. Anticoccidial Drugs: Lesion Scoring Techniques in Battery and Floor-Pen Experiments with Chickens. Experimental Parasitology 28, 30-36.

Kheirabadi KP, Katadj JK, Bahadoran S, Teixeirada Silva JA, Samani AD, Bashi MC. 2014. Comparison of the anticoccidial effect of granulated extract of Artemisia sieberi with monensin in experimental coccidiosis in broiler chickens. Experimental Parasitology 141, 129–133.

Koinarski V, Georgieva N, Gadjeva. 2005. Antioxidant status of broiler chickens infected with Eimeria acervulina. Revue de Médicine Véterinaire 156, 498–502.

Lawal HO, Etatuvie SO, Fawehinmi AB. 2012. Ethnomedicinal and pharmacological properties of Morinda lucida. Journal of Natural Products 5, 93–9.

Long PL. 1982. The biology of Coccidia. University Park Press, Baltimore.

Loredana P, Adriana G, Alexandru F, Tǎbǎranb M, Oana D, Zsuzsa K, Cristian M. 2015. Effects of artemisinin in broiler chickens challenged with Eimeria acervulina, E. maxima and E. tenella in battery trials. Veterinary Parasitology 214(3), 264-271.

Mathis GF, Broussard C. 2006. Increase level of Eimeria sensitivity to Diclarzuryl after using a live coccidial vaccine. Avian Diseases 50(3), 321-324.

McCann M, Newell E, Preston C, Forbes K. 2006. The use of man nan- oligosaccharides and/or tannin in broiler diets. International Journal of Poultry Sciences 5, 873–879.

McDougald LR. 2003. In: Diseases of Poultry 11th edition, Y. M. Saif (eds) Iowa State press, Ames, IA USA.

Mortier L, Huet AC, Charlier C, Daeseleire E, Delahaut P, Van Peteghem C. 2005. Incidence of residues of nine anticoccidials in eggs. Food Additive Contamination 22, 1120-1125.

Mouzouvi R, Djègo JG, Sehonou J, Lalèyè A, Priuli F, Bigot A. 2014. Effet de l’association Combretum micranthum G. Don (Combretaceae) et Cochlospermum tinctorium A. Rich. (Cochlospermaceae) dans la prise en charge de l’hépatite virale B. Médecine Traditionelle Africaine 17(1), 10-14.

Ogunlana OE, Ogunlana O, Farombi OE. 2008. Morinda lucida: Antioxidant and reducing activities of crude methanolic stem bark extract. Advanced Natural Applied Sciences 2, 49–54.

Oh H, Youn H, Noh J, Jang D, Kang Y. 1995. “Anticoccidial effects of artemisinin on the Eimeria tenella”. Korean Journal of Veterinary Research 35, 123–130.

Ola-Fadunsin SD, Ademola IO. 2014. Anticoccidial effects of Morinda lucida acetone extracts on broiler chickens naturally infected with Eimeria species. Pharmaceutical Biology 52(3), 330-334.

Peek H, Landman W. 2006. Resistance to anticoccidial drugs of ducth avian Eimerian spp. International Journal of Parasitology 25, 1331-1351.

Peek H. 2010. Resistance to anticoccidial drugs: alternative strategies to control coccidiosis in broilers. PhD Dissertation, University Utrecht, the Netherlands, p 67.

Perry B, Randolph T, McDermott J, Sones K, Thornton P. 2002. Investing in Animal Health Research to Alleviate Poverty. ILRI (International Livestock Research Institute), Nairobi, Kenya.

Shazia A. 2013. Evaluation of anticoccidial activity of aqueous extract of Fomes fomentarius. New York Science Journal 6(8).

Shirley MW, Smith AL, Blake DP. 2007. Challenges in the successful control of avian coccidian. Vaccine 25, 5540–5547.

Smil V. 2002. Worldwide transformation of diets, burdens of meat production and opportunities for novel food proteins. Enzyme Microbe Techniques 30, 305–311.

Sørensen JT, Edwards S, Noordhuizen J, Gunnarson S. 2006. Animal production system in the industrialized world. Scientific and technical Review. OIE 25(2), 493–503.

Spiegler V, Liebau E, Peppler C, Raue K, Werne S, Strube C, Heckendorn F, Agyare C, Stark T, Hofmann T, Hensel A. 2016. A Hydroalcoholic Extract from Paullinia pinnata L. Roots Exerts Anthelmintic Activity against Free-Living and Parasitic Nematodes. Planta Medica 82, 1173 –1179.

Sujikara l. 2000. Andrographis paniculata A paper presented at an International Conference on Tropical Agriculture for better health and environment at Kasetsart, University, ampaengsaen, Nakornpathom, Thailand p 7.

Soulsby EJL. 1986. Helminths, Arthropods and Protozoa of domestic Animals. 7th Edn., Beiliere Tindal II, London. p 231.

Tewari A, Maharana B. 2011. Control of poultry coccidiosis: changing trends. Journal of Parasitic Diseases 35, 10–17.

Tokoudagba JM, Gandonou CD, Houngue U, Auger C, Schini-Kerth VB. 2018. Phytochemistry and Vasorelaxant activity of some plants used traditionnaly against high blood pressure in Benin. World Journal of Pharmacology Sciences 6(10), 40-48.

Williams RB. 1999. A compartmentalized model for the estimation of the cost of coccidiosis to the world’s chicken production industry. International Journal for Parasitology 29, 1209-1229.

Williams RB, Marshall RN, Pages M, Dardi M, DelCacho E. 2009. Pathogenesis of Eimeria praecox in chickens: virulence of field strains compared with laboratory strains of E. praecox and Eimeria acervulina. Avian Pathology 38, 359–366.

Youn HJ, Noh JW. 2001. Screening of the anticoccidial effect of herb extracts against Eimeria tenella. Veterinary Parasitology 96, 257-263.