Isolation and characterization of indigenous Rhizobacteria for control of Phytophthora in Red Chili plants and its use as Rhizobacteria management of plant growth
Paper Details
Isolation and characterization of indigenous Rhizobacteria for control of Phytophthora in Red Chili plants and its use as Rhizobacteria management of plant growth
Abstract
Chili is one of the horticultural commodities that still requires serious handling, especially in the context of increasing production both in quality and quantity. Seed treatment using biological seed treatment agents is an alternative as a substitute for synthetic chemicals in seed treatment. This study aims to determine the inhibitory power of rhizobacteria in the growth of colonies of pathogens carried by chili seeds in vitro and the mechanism of action of rhizobacteria to inhibit the growth of Phytophthora colonies in chili. The research was carried out at the Seed Science and Technology Laboratory, Laboratory of Plant Disease, Biology Laboratory, Teacher Training and Education Faculty, Syiah Kuala University, Inter-University Central Laboratory and Bacteriology Laboratory of IPB Bogor from July 2014 to March 2015. Conducted isolation of carrying pathogens of chili seeds namely pathogenic fungi P. Capsi and isolation of rhizobacteria of bio-control agent candidates and rhizobacterial agents promote plant growth candidates. The results showed that the results of detection, exploration and isolation of rhizobacteria from the root system of healthy chili plants obtained 154 rhizobacterial isolates. Antagonistic test results obtained one rhizobacterial isolate, namely Rbks-5 isolate has a very high inhibitory power on the growth of pathogenic colonies test S.rolfsii dan Phytium sp. While rhizobacteria Rbks-6 isolates and Rbks-7 isolates have very high inhibitory power on the pathogen S. rolfsii and P. capsici. Rhizobacterial isolates which produced the highest IAA were Rbkb-2 isolates, followed by 3 isolates capable of producing IAA growth regulators namely Rbkp-4, Rbks-2, Rbks-5 rhizobacterial isolates.
Agrios GN. 1997. Plant Pathology, 4 th ed. Academic Press, New York USA.
Bae YS, Park KS, Lee YG, Choi OH. 2007. A simple and rapid methode for functional analysis of plant growth-promoting rhizobacteria using the development of cucumber adventious root system. Plant Pathol. J. 23(3), 223-225.
Baharum SN, Salleh AB, Razak CNA, Basri M, Rahman MBA, Rahman RNZRA. 2003. Organic solvent tolerant lipase by Pseudomonas sp. strain S5: stability of enzym in organic solvent and physical factors affecting its production. Ann Microbiol 53, 75-83.
Bai Y, Pan B, Charles TC, Smith DL. 2002. Co-inoculation dose and root zone temperatur for plant growth promoting rhizobacteria on soybean (Glycine max L. Merr) grown in soil less media. Soil Biol Biochem 34, 1953-1957.
Bennet, MA. 2002. Application of biologicals to enhance vegetable seed production and quality.
Brandle F. 2001. Seed Treatment: Evolving to Achieve Crop Genetict Potential. In: Biddle, A.J. (ed) Seed Treatment:Callenges & Opportunities. BCPC Symposium Proceedings 76p.
Cook RJ, Baker KF. 1983. The nature and practice of biological control of plant pathogens. The American Phytopathological Society, St.Paul, MN USA.
Kumar NR, Arasu VT, Gunasekaran P. 2002. Genotypeing of antifungal compounds producing plant growth-promoting rhizobacteria, Pseudomo-nas fluorescens.Current Science 82, 1463-1466.
Lugtenberg BJ, Chin-A-Woeng TF, Bloemberg GV. 2002. Microbe-plant interactions: principles and mechanisms. Antonie van Leeuwenhoek 81, 373-383.
Maude RB. 1996. Seedborne Diseases and Their Control, Priciples and Practice. CAB International. Wallingford UK. 245p.
Pieterse CMJ, Van Wees SCM, Ton JL, Van Pelt JA, Van Loon LC. 2002. Signalling in rhizobacteria-induced systemic resistance in Arabidopsis thaliana. Plant Biol 4(5), 535-544.
Ramamoorthy V, Raguchander T, Samiyappan R. 2002. Induction of defence-related protein in tomato roots treated with Pseudomonas flurescens Pfi and Fusarium oxysporum f.sp. lycopersici. Plant & Soil 239, 55-68.
Shen SS, Choi OH, Lee SM, Park CS. 2002a. In vitro and in vivo activities of biocontrol agent, Serratia plymuthica A21-4, against Phytophthora capsici. J. Plant Pathol 18(4), 221-224.
Shen SS, Kim JW, Park CS. 2002b. Serratia plymuthica Strain A21-4: a potential biocontrol agenst against Phytophthora capsici of pepper. Plant Pathology Journal 18(3), 138-141.
Siddiqui AZ. 2005. PGPR : Prospective biocontrol agents of plant pathogens. In: Siddiqui, Z.A. (Ed.), PGPR: Biocontrol and Biofertilization. Springer, Dordrecht p. 197-216.
Sutariati GAK. 2006. Seed treatment with biocontrol agents for anthracnose disease control and increased yield and quality of chili seeds. [Dissertation]. Postgraduate School, Bogor Agricultural University, Bogor 163p.
Syamsuddin, Ilyas S, Manohara D, Sudarsono. 2007. The inhibitory effectiveness of vegetable oils on the growth of colonies of several pathogens carried by chili seeds in vitro. Agrista 11(2), 81-91.
Szczech M, Shoda M. 2004. Biocontrol of Rhizoctonia damping-off of tomato by Bacillus subtilis combined with Bukholderia cepacia. J. Phytopathol 152, 549-556.
Yan Z, Reddy MS, Ryu CM, Mc.Inroy JA, Wilson M, Kloepper JW. 2002. Induced systemic protection against tomato late blight elicited by palnt growth-promoting rhizobacteria. Phytopathology 92, 1329-1333.
Ezha Dinda Merianzha, Syamsuddin, Marlina (2019), Isolation and characterization of indigenous Rhizobacteria for control of Phytophthora in Red Chili plants and its use as Rhizobacteria management of plant growth; JBES, V14, N1, January, P257-265
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