Isolation and identification of endophytic fungi and its potential as a pharmacological activities

Paper Details

Research Paper 13/09/2024
Views (13) Download (4)
current_issue_feature_image
publication_file

Isolation and identification of endophytic fungi and its potential as a pharmacological activities

A. Lakshmi, K. Kanimozhi, V. Ambikapathy, A. Panneerselvam
J. Bio. Env. Sci.25( 3), 217-228, September 2024.
Certificate: JBES 2024 [Generate Certificate]

Abstract

The present investigation deals with the isolation and identification of endophytic fungi from medicinal plants such as Carica papaya and Melia dubia leaves, the biosynthesis of silver nanoparticles and the pharmacological activities of potential fungi. The global issue of drug-resistant microbes and their impact on public health is a significant concern, necessitating the urgent development of new and more potent antimicrobial treatments. The endophytic fungi like Aspergillus niger, A. flavus, A. terreus, A. fumigatus, Curvularia sp., C. lunata, Fusarium sp., F.oxysporum, Helminthosporium sativum, Nigrospora sphaeria, Pencillium janthinellum, Trichoderma sp. and T. viride were recorded from C. papaya and M. dubia leaves. The maximum number of colonies seen in A. niger, A. flavus and T. viride was recorded from medicinal plant leaves. The potential endophytic fungi such as A. niger, A. flavus and T. viride were analyzed for bioactive compounds like alkaloids, aminoacids, carbohydrates, coumarins, flavonoids, phenols, proteins, quinones, saponins, steroids, tannins, terpenoids and triterpenoids. The antioxidant and anti-inflammatory activity of secondary metabolites of different concentrations of endophytic fungi were analyzed. The agar well diffusion method to analyze the UV-Vis spectra of silver nanoparticles and the zone of inhibition in bacterial and fungal organisms from potential endophytic fungi. These findings suggested that different parts of papaya fruits harbour an array of fungal endophytes that could be important agents in attributing the high nutritive status to the fruit and can serve as potent microbial cocktails for developing value-added fermented products of this important fruit.

VIEWS 9

Boham BA, Kocipai AR. 1994. Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium reticulatum and V. calycinum. Pacific Science 48, 458-63.

Brand-Williams W, Zuvelier ME, Berset CLWT. 1995. Use of a free radical method to evaluate antioxidant activity. LWT – Food Science and Technology 28, 25-30.

Chandra H, Kumari P, Yadav A, Yadav D. 2022. Green biosynthesized metallic nanoparticles and future biomedical applications. In: Shanker U, Hussain CM, Rani M (Eds.), Green Nanomaterials for Industrial Applications, Micro and Nano Technologies, Elsevier, 93-105.

Chandra H, Yadav A, Prasad R, Kalra SJS, Singh A, Bhardwaj N, Gupta KK. 2024. Fungal endophytes from medicinal plants acting as natural therapeutic reservoir. The Microbe 3, 100073.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2(1), S178-80.

Domsch KH, Gamas W, Anderson TH. 1980. Compendium of Soil Fungi, Vol. I. Academic Press, New York, 205-205.

El-Esawi MA, Elkelish A, Soliman M, Elansary HO, Zaid A, Wani SH. 2020. Serratia marcescens BM1 enhances cadmium stress tolerance and phytoremediation potential of soybean through modulation of osmolytes, leaf gas exchange, antioxidant machinery and stress-responsive gene expression. Antioxidants.

Ellis MB, Ellis J. 1976. More Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, Surrey, UK.

Ellis MB. 1971. Dematiaceous Hyphomycetes. Kew, Surrey: Commonwealth Mycological Institute, 1-608.

Gillman JC. 1957. A Manual of Soil Fungi Revised 2nd edition. Oxford and IBH Publishing Company (Indian reprint), Calcutta, Bombay, New Delhi, 436.

Gond SK, Verma VC, Kumar A, Kumar V, Kharwar RN. 2007. Study of endophytic fungal community from different parts of Aegle marmelos Correae (Rutaceae) from Varanasi (India). World Journal of Microbiology and Biotechnology 23(10), 1371-1375.

Gurgel RS, Pereira DI, Garcia A, Souza A, Silva, Andrade C, Silva W, Nunez C, Fantin C, Procopio R, Albuquerque P. 2023. Antimicrobial and antioxidant activities of endophytic fungi associated with Arrabidaea chica (Bignoniaceae). Journal of Fungi (Basel) 9(8), 864.

Gurunathan S, Raman J, Abd Malek SN, John PA, Vikineswary S. 2013. Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: A potential cytotoxic agent against breast cancer cells. International Journal of Nanomedicine 8, 4399-4413.

Harborne JB. 1973. Phytochemical methods: A guide to modern techniques of plant analysis. 2nd ed. London, New York.

Khalil AMA, Hassan SE-D, Alsharif SM, Eid AM, Ewais EE-D, Azab E, Gobouri AA, Elkelish A, Fouda A. 2021. Isolation and characterization of fungal endophytes isolated from medicinal plant Ephedra pachyclada as plant growth-promoting. Biomolecules 11(2), 140.

Kohlmeyer J, Kohlmeyer E. 1979. Marine Mycology: The Higher Fungi. Academic Press, New York.

Kumala P, Poppy. 2006. Dorland Medical Dictionary. EGC Medical Book Publisher, Jakarta.

Mbilu M, Wanyoike W, Kangogo M, Bii C, Agnes M, Kihia C. 2018. Isolation and characterization of endophytic fungi from medicinal plant Warburgia ugandensis. Journal of Biology, Agriculture and Healthcare 12, 57-66.

Moharram AM, Zohri AA, Omar HM, Abd El-Ghani OA. 2017. In vitro assessment of antimicrobial and anti-inflammatory potential of endophytic fungal metabolites extracts. European Journal of Biological Research 7(3), 234-244.

Muthukrishnan S, Prakathi P, Sivakumar T, Thiruvengadam M, Jayaprakash B, Baskar V, Rebezov M, Derkho M, Zengin G, Shariati MA. 2022. Bioactive components and health potential of endophytic micro-fungal diversity in medicinal plants. Antibiotics 11(11), 1533.

Obadoni BO, Ochuko PO. 2001. Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta states of Nigeria. Global Journal of Pure and Applied Sciences 8, 203-208.

Packia LM, Poulpriya K, Mohan VR. 2013. In vitro antioxidant activity of Avicennia marina (Forssk.) Vierh pneumatophore (Avicenniaceae). Science Research Reporter 3(2), 106-114.

Perez C, Pauli M, Bazerque P. 1990. An antibiotic assay by agar well diffusion method. Acta Biologiae et Medicinae Experimentalis 15, 113-115.

Petrini O, Fisher PJ. 1986. Fungal endophytes in Salicornia perennis. Transactions of the British Mycological Society 87(4), 647–651.

Rafi MM, Krishnaveni M, Charyulu P. 2019. Recent developments in applied microbiology and biochemistry. Elsevier; Amsterdam, The Netherlands: Phosphate-solubilizing microorganisms and their emerging role in sustainable agriculture, 223–233.

Raper KB, Thom C. 1949. A manual of Penicillia. Williams and Wilkins Co., Baltimore, Md., U.S.A.

Sardul SS, Suneel K, Ravindra PA. 2014. Isolation and identification of endophytic fungi from Ricinus communis Linn. and their antibacterial activity. International Journal of Research in Pharmacy and Chemistry 4(3), 611–618.

Singh S, Singh RP. 2008. In vitro methods of assay of antioxidants: an overview. Food Reviews International 24, 392-415.

Singh T, Jyoti K, Patnaik A, Singh A, Chauhan R, Chandel SS. 2017. Biosynthesis, characterization, and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus. Journal of Genetic Engineering and Biotechnology 15(1), 31-39.

Soliman M, Elkelish A, Souad T, Alhaithloul H, Farooq M. 2020. Brassinosteroid seed priming with nitrogen supplementation improves salt tolerance in soybean. Physiology and Molecular Biology of Plants 26, 501–511.

Strobel GA, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbial Molecular Biology Reviews 67, 491-502.

Tsipinana S, Husseiny S, Alayande KA, Raslan M, Amoo S, Adeleke R. 2023. Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants. Frontiers in Plant Science 14, 01-20.

Van-Burden TP, Robinson WC. 1981. Formation of complexes between proteins and tannin acid. Journal of Agricultural and Food Chemistry 1, 77.

Zeb A, Ullah F. 2016. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. Journal of Analytical Methods in Chemistry, 9412767.