Isolation and identification of endophytic fungi and its potential as a pharmacological activities
Paper Details
Isolation and identification of endophytic fungi and its potential as a pharmacological activities
Abstract
The present investigation deals with the isolation and identification of endophytic fungi from medicinal plants such as Carica papaya and Melia dubia leaves, the biosynthesis of silver nanoparticles and the pharmacological activities of potential fungi. The global issue of drug-resistant microbes and their impact on public health is a significant concern, necessitating the urgent development of new and more potent antimicrobial treatments. The endophytic fungi like Aspergillus niger, A. flavus, A. terreus, A. fumigatus, Curvularia sp., C. lunata, Fusarium sp., F.oxysporum, Helminthosporium sativum, Nigrospora sphaeria, Pencillium janthinellum, Trichoderma sp. and T. viride were recorded from C. papaya and M. dubia leaves. The maximum number of colonies seen in A. niger, A. flavus and T. viride was recorded from medicinal plant leaves. The potential endophytic fungi such as A. niger, A. flavus and T. viride were analyzed for bioactive compounds like alkaloids, aminoacids, carbohydrates, coumarins, flavonoids, phenols, proteins, quinones, saponins, steroids, tannins, terpenoids and triterpenoids. The antioxidant and anti-inflammatory activity of secondary metabolites of different concentrations of endophytic fungi were analyzed. The agar well diffusion method to analyze the UV-Vis spectra of silver nanoparticles and the zone of inhibition in bacterial and fungal organisms from potential endophytic fungi. These findings suggested that different parts of papaya fruits harbour an array of fungal endophytes that could be important agents in attributing the high nutritive status to the fruit and can serve as potent microbial cocktails for developing value-added fermented products of this important fruit.
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