Isolation and identification of lipase producing bacteria from coal mines of Darra Adam Khel, Khyber Pakhtoonkhwa, Pakistan

Paper Details

Research Paper 01/08/2015
Views (304) Download (9)
current_issue_feature_image
publication_file

Isolation and identification of lipase producing bacteria from coal mines of Darra Adam Khel, Khyber Pakhtoonkhwa, Pakistan

Fahad Ali, Sulaiman Bahadar, Khalil Ur Rahman, Ilyas Khan,Aurang Zeb, Javid khan, Muhammad Daud, Adil Hassan, Nasir Khan Rabbani, Aamer Ali Khattak, Faisal Khan Jadoon, Azam Hayat
Int. J. Biosci.7( 2), 187-191, August 2015.
Certificate: IJB 2015 [Generate Certificate]

Abstract

Lipase producing bacteria are widespread in nature and play a key role in our daily lives. These bacteria have the capability to produce lipase enzyme which have many industrial applications. Among the various lipases, bacterial are the most significant as compared to animal and fungal lipases. The aim of current study was to isolate and to identify lipase producing bacteria. Different coal samples were collected from different areas of Darraa Adam Khel Kohat, Khyber Pakhtunkhwa, Pakistan. A total of 14 isolates were recovered from these coal samples. Samples were serially diluted and were cultured for further processing. Identification of lipolytic isolates was carried out on 1 percent tri-butarine added into the Luria Brutenia (LB) medium. Lipase producing was identified having clear zones around their colonies. Identified isolates were subjected to the gram staining and biochemical test like Oxidase Test, Catalase Test, Motility Indol Ureas (MIU) Test, Triple sugar iron (TSI) Test and Simmon Citrate Test.

VIEWS 21

Akanbi T, Zaman A, Bakar F. 2010. “Highly thermostable extracellular lipase-producing Bacillus strain isolated from a Malaysian hotspring and identified using 16S rRNA gene sequencing”. International Food Research Journal (17), 45-53.

Aravindan R, Anbumathi P, Viruthagiri T. 2006. “Lipase applications in food industry” Indian Journal of Biotechnology (6), 141-158.

Arpigny JL, Jaeger KE. 1999. Bacterial lipolytic enzymes: classification and propertie. Biochemistry Journal 343, 177-183.

Begam MS, Pradeep FS, Pradeep BV. 2012. Production, purification, characterization and applications of lipase from serratia marcescens”.Asian Journal of Pharma and Clinical Research 5, 237-245.

Falch EA. 1991. Industrial enzymes-developments in production and application. Biotechnology Advances. 9, 643-658.

Fenga W, Wangb XQ, Zhouc W, Liud GY, Wane YJ. 2001. Isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, Bombyx mori, reared on different forage”. Journal of Insect Science 11, 131-135.

Grbavcic SZ, Dimitrijevic-Brankovic SI, Bezbradica DI, Siler- Marinkovic SS, Knezevic ZD. 2007. Effect of fermentation conditions on lipase production by Candida utilis. Journal of the Serbian Chemical Society 72(8–9), 757–65.

Gupta R, Gupta N, Rathi P. 2004. Bacterial lipases: an overview of production, purification and biochemical properties. Applied Microbiology and Biotechnology 64, 763-781.

Gupta N, Shai V, Gupta R. 2007. Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochemistry 42(2), 518– 526.

Hasan F, Shah AA, Javed S, Hameed A. 2010. Enzymes used in detergents: Lipases. African Journal of Biotechnology 9(31), 4836-4844.

Joseph B, Ramteke, PW, Thomas G. 2008. Cold active microbial lipases: Some hot issues and recent developments. Biotechnology Advances 26, 457-470.

Nadeem UM, Daud Hidayatullah, Muhammad S, AyşegülTÖ, Sami U, Muhammad Q. 2015. Screening Identification and Characterization of LipaseProducing Soil Bacteria From Upper Dir And Mardan KhyberPakhtunkhwa, Pakistan. International Journal of Biosciences 6(2), 49-55. http://dx.doi.org/10.12692/ijb/6.4.106-111

Park H, Lee K, Chi Y, Jeong S. 2005. Effects of methanol on the catalytic properties of porcine pancreatic lipase. Journal of Microbiology and Biotechnology 15(2), 296–301.

Saxena RK, Sheoran A, Giri B, Davidson WS. 2003.  Purification  strategies  for  microbial  lipases. Journal of Microbiological Methods 52, 1 – 18.

Sharmaa R, Chistib Y, Banerjeea UC. 2001. Production, purification, characterization, and applications of lipases. Biotechnology Advances 19, 627–662.

Svendsen A. 1994. Sequence comparisons within the lipase family. In: Woolley, P., Petersen, S.B. (Eds.), Lipases: Their Structure, Biochemistry and Applications. Cambridge Univ. Press, Cambridge, 1– 21.

Veerapagu M, Narayanan AS, Ponmurugan K, Jeya KR. 2013.Screening selection identification production and optimization of bacterial lipase from oil spilled soil. Asian Journal of Pharma and Clinical Research 6(3), 62-67.

Rohit S, Yusuf C, Uttam C. 2001. Production, purification, characterization, and applications of lipases. Biotechnology Advances 19, 627 –662.

Fariha H, Aamer AS, Sundus J, Abdul H. 2010. Enzymes used in detergents: Lipases, African Journal of Biotechnology 9(31), 4836-4844.