Isolation and Identification of Salmonella sp. from different food

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Research Paper 01/02/2016
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Isolation and Identification of Salmonella sp. from different food

Nasima Aktar, Rabeya Bilkis, Mohammad Ilias
Int. J. Biosci.8( 2), 16-24, February 2016.
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The consumption of meat as well as dry fish and many different types of processed foods are dramatically increasing in recent years. Although the productions are increasing, the quality is not maintained up to standard. The microbial safety status of raw meat, dry fish and pudding was investigated in this study to determine the incidence of Salmonella sp. contamination. Isolation and identification of Salmonella sp. by conventional tests (microscopy and culture in differential media combined with several biochemical tests) was performed. Presence of Salmonella sp. in the foods indicates fecal contamination.


AOAC INTERNATIONAL. 2000. Official Methods of Analysis, 17th Edition. Methods  967.25-967.28, 978.24, 989.12, 991.13, 994.04, and 995.20. AOAC INTERNATIONAL, Gaithersburg, MD.

Aseel AS, Mayada FH, Majed HM. 2013. Isolation and Molecular Identification of Salmonella typhimurium from Chicken Meat in Iraq. J. World’s Poult. Res. 3(2), 63-67.

Baumler AJ, Tsolis RM, Heffron F. 2000. Virulence mechanisms of Salmonella sp. and their genetic basis. In: C Wray and A Wray (eds), Salmonella sp. in Domestic Animals, (CAB International, Wallingford, UK), 52-57.

BirolÖzkalp B. Isolation and Identification of Salmonellas from Different Samples; Department of Medicinal Laboratory Vocational School of Health Services of Selçuk University, Konya Turkey.

Centers for Disease Control   and    Prevention. 2012. National Center for Environmental Health Vessel Sanitation Program. Health Practices on Cruise Ships: Training for Employees.

Cross T, Bazron B, Dennis K, Isaacs M. 1989. Towards A Culturally Competent System of Care, Volume I. Washington, DC: George Town University Child Development Center, CASSP Technical Assistance Center.

Grimont PAD, Grimont F, Bouvet P. 2000. Taxonomy of the genus Salmonella sp.

Hammack TS, Amaguana RM, June GA, Sherrod PS, Andrews WH. 1999. Relative effectiveness of selenite Cysteine broth, tetrathionate broth, and Rappaport-Vassiliadis medium for the recovery of Salmonella from foods with a low microbial load. J. Food Prot. 62, 16-21.

June GA, Sherrod PS, Hammack TS, Amaguana RM, Andrews WH. 1995. Relative effectiveness of selenite cysteine broth, tetrathionate broth, and Rappaport-Vassiliadis medium for the recovery of Salmonella sp. from raw flesh and other highly contaminated foods: Precollaborative study. J. AOAC Int. 78, 375-380.

Ricke SC, Pillai SD, Norton RA, MAciorowski KG, Jones FT. 1998. Applicability of rapid methods for detection of Salmonella sp. in poultry feeds: a review. Journal of Rapid Methods and automation in Microbiology 6, 239-258.

Smith JL. 1994. Arthritis and food borne bacteria. Journal of Food Protection 57, 935-941.

Tauxe RV. 1991. Salmonella: a postmodern pathogen. Journal of Food Protection 54, 563-568.

Williams JE. 1981. Salmonellas in poultry feed—a worldwide review. Part II: Methods in isolation and identification. World’s Poult. Sci. J. 37, 19–25.